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The map label for this gene is hisS

Identifier: 116492878

GI number: 116492878

Start: 1115213

End: 1116487

Strand: Reverse

Name: hisS

Synonym: PEPE_1116

Alternate gene names: 116492878

Gene position: 1116487-1115213 (Counterclockwise)

Preceding gene: 116492880

Following gene: 116492877

Centisome position: 60.93

GC content: 36.86

Gene sequence:

>1275_bases
ATGAAGTATCAAAAACCAAAGGGAACAGCAGACATATTACCGCCATTTTCAAAAGAATGGCAATTTGTTGAACAGAATGC
TCGCGAAACATTTGCACTTTATAACTACGAAGAAATCCGTACACCAATTTTTGAAAAATTTGAAGTCTTTTCAAGAAGTG
CTGGGGATACATCTGATATTGTTACCAAAGAAATGTATGATTTTGATGATAAAGGTGGACGCCACATTGCGTTACGTCCA
GAAGGAACAGCCGGAGTGGTTCGGGCATTTGTTGAAAATAAGTTATATGGACCAGAACACCAGAAACCGGTAAAAGTTTA
CTACATGGGACCAATGTTCCGTTATGAACGTCCACAATCTGGCCGACTACGTGAATTTCACCAAATCGGGGTAGAAGCAT
TCGGTAGTGATAGCCCTAAAATTGACGTTGAAACAATCATGATGGGGATGGATTTTCTTAAAAAGTTGAAGGTTTCGGGA
CTAAAATTAGTTATCAATACGCTAGGTGATAAAGAGAGTCGTGATCGTTACCGCCAAGCATTGATTGATTATTTGGAACC
CCATTTTGAAGAATTGAGTGATGATTCTAAAGCTCGTCTCCATAAAAATCCTTTACGTGTTCTAGATAGCAAAGATAAAA
ACGATCAAAAGATTGTTGAAAATGCTCCGGAGATTTTAGACTTCTTAACTGAAGATGCTCAAAAGCACTTTACAAGTGTT
AAAGAAGAACTTGATACATTAGGAGTAGACTACGTTGTGGATTCATCCATGGTTCGTGGGTTGGACTATTATAACCATAC
TATTTTTGAAATTATGATTGCAGATTCTCCTTTAGGAAAGGGAGATGTTACTATCTGTGCTGGAGGTCGTTATAATGGTT
TGGTTGAAGAATTAGGAGGACCTGAAGTTTCTGGAGTTGGTTTTGGACTAGGAGTTGAACGTCTGCTCTTATTATTAAAT
GCTGAAACACAAACCACTCTTCAAAGTAAACAACTTGATTTTTACGTTGTTGGAATTGGCGATCTTGTTCAAAATGATGT
TCTTAAGGTTGTCCATGAACTTCGTCAAATGAATTTTGTAACTGAACAAGATTACCTTGATCGTAAACCAAAAGCTCAAT
TTAAATCAGCGGATAAGCTGAACGCTAAGTATGTGGTTACCATTGGCGAATCTGAAATGAATGATCGAATCTTTAAATTA
AAGGACATGCATTCTGGAGAAGAACGTACCGTAGCATTAAGCGAAATTAATACATTAAAAGATCTTTTAAAATAA

Upstream 100 bases:

>100_bases
TGCCGTAGAGTACAAATCTAAGGTGGTACCGCGTCAAGACGCCCTTGTTAAATCAAGGGCGTCTTTTATTTTCTTTGAAA
AATATTTGAGGTGAATATAA

Downstream 100 bases:

>100_bases
GCGAGGAAGACTAATGAAAAGAACAACATACGCTGGTTTAGTAAATGAACAGTATACTGGCCAAACAGTAACTTTAAAAG
GATGGGTTCAAAAACGTCGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKYQKPKGTADILPPFSKEWQFVEQNARETFALYNYEEIRTPIFEKFEVFSRSAGDTSDIVTKEMYDFDDKGGRHIALRP
EGTAGVVRAFVENKLYGPEHQKPVKVYYMGPMFRYERPQSGRLREFHQIGVEAFGSDSPKIDVETIMMGMDFLKKLKVSG
LKLVINTLGDKESRDRYRQALIDYLEPHFEELSDDSKARLHKNPLRVLDSKDKNDQKIVENAPEILDFLTEDAQKHFTSV
KEELDTLGVDYVVDSSMVRGLDYYNHTIFEIMIADSPLGKGDVTICAGGRYNGLVEELGGPEVSGVGFGLGVERLLLLLN
AETQTTLQSKQLDFYVVGIGDLVQNDVLKVVHELRQMNFVTEQDYLDRKPKAQFKSADKLNAKYVVTIGESEMNDRIFKL
KDMHSGEERTVALSEINTLKDLLK

Sequences:

>Translated_424_residues
MKYQKPKGTADILPPFSKEWQFVEQNARETFALYNYEEIRTPIFEKFEVFSRSAGDTSDIVTKEMYDFDDKGGRHIALRP
EGTAGVVRAFVENKLYGPEHQKPVKVYYMGPMFRYERPQSGRLREFHQIGVEAFGSDSPKIDVETIMMGMDFLKKLKVSG
LKLVINTLGDKESRDRYRQALIDYLEPHFEELSDDSKARLHKNPLRVLDSKDKNDQKIVENAPEILDFLTEDAQKHFTSV
KEELDTLGVDYVVDSSMVRGLDYYNHTIFEIMIADSPLGKGDVTICAGGRYNGLVEELGGPEVSGVGFGLGVERLLLLLN
AETQTTLQSKQLDFYVVGIGDLVQNDVLKVVHELRQMNFVTEQDYLDRKPKAQFKSADKLNAKYVVTIGESEMNDRIFKL
KDMHSGEERTVALSEINTLKDLLK
>Mature_424_residues
MKYQKPKGTADILPPFSKEWQFVEQNARETFALYNYEEIRTPIFEKFEVFSRSAGDTSDIVTKEMYDFDDKGGRHIALRP
EGTAGVVRAFVENKLYGPEHQKPVKVYYMGPMFRYERPQSGRLREFHQIGVEAFGSDSPKIDVETIMMGMDFLKKLKVSG
LKLVINTLGDKESRDRYRQALIDYLEPHFEELSDDSKARLHKNPLRVLDSKDKNDQKIVENAPEILDFLTEDAQKHFTSV
KEELDTLGVDYVVDSSMVRGLDYYNHTIFEIMIADSPLGKGDVTICAGGRYNGLVEELGGPEVSGVGFGLGVERLLLLLN
AETQTTLQSKQLDFYVVGIGDLVQNDVLKVVHELRQMNFVTEQDYLDRKPKAQFKSADKLNAKYVVTIGESEMNDRIFKL
KDMHSGEERTVALSEINTLKDLLK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=467, Percent_Identity=26.1241970021413, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI6996014, Length=458, Percent_Identity=27.0742358078603, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1788861, Length=426, Percent_Identity=41.0798122065728, Blast_Score=331, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI71993693, Length=469, Percent_Identity=27.0788912579957, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71993686, Length=469, Percent_Identity=27.0788912579957, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6325290, Length=452, Percent_Identity=24.3362831858407, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_PEDPA (Q03F51)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804613.1
- ProteinModelPortal:   Q03F51
- SMR:   Q03F51
- STRING:   Q03F51
- GeneID:   4417051
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1116
- NMPDR:   fig|278197.10.peg.980
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q03F51
- BioCyc:   PPEN278197:PEPE_1116-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48353; Mature: 48353

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQKPKGTADILPPFSKEWQFVEQNARETFALYNYEEIRTPIFEKFEVFSRSAGDTSDI
CCCCCCCCCCCCCCCCCHHHHHHHHCCHHHEEEECHHHHHCHHHHHHHHHHCCCCCHHHH
VTKEMYDFDDKGGRHIALRPEGTAGVVRAFVENKLYGPEHQKPVKVYYMGPMFRYERPQS
HHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC
GRLREFHQIGVEAFGSDSPKIDVETIMMGMDFLKKLKVSGLKLVINTLGDKESRDRYRQA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
LIDYLEPHFEELSDDSKARLHKNPLRVLDSKDKNDQKIVENAPEILDFLTEDAQKHFTSV
HHHHHHHHHHHHCCCHHHHHHCCCHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
KEELDTLGVDYVVDSSMVRGLDYYNHTIFEIMIADSPLGKGDVTICAGGRYNGLVEELGG
HHHHHHCCCHHHCCHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHHCCC
PEVSGVGFGLGVERLLLLLNAETQTTLQSKQLDFYVVGIGDLVQNDVLKVVHELRQMNFV
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC
TEQDYLDRKPKAQFKSADKLNAKYVVTIGESEMNDRIFKLKDMHSGEERTVALSEINTLK
CCHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCCCHHEEECCCCCCCCCEEEHHHHHHHH
DLLK
HHHC
>Mature Secondary Structure
MKYQKPKGTADILPPFSKEWQFVEQNARETFALYNYEEIRTPIFEKFEVFSRSAGDTSDI
CCCCCCCCCCCCCCCCCHHHHHHHHCCHHHEEEECHHHHHCHHHHHHHHHHCCCCCHHHH
VTKEMYDFDDKGGRHIALRPEGTAGVVRAFVENKLYGPEHQKPVKVYYMGPMFRYERPQS
HHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC
GRLREFHQIGVEAFGSDSPKIDVETIMMGMDFLKKLKVSGLKLVINTLGDKESRDRYRQA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
LIDYLEPHFEELSDDSKARLHKNPLRVLDSKDKNDQKIVENAPEILDFLTEDAQKHFTSV
HHHHHHHHHHHHCCCHHHHHHCCCHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
KEELDTLGVDYVVDSSMVRGLDYYNHTIFEIMIADSPLGKGDVTICAGGRYNGLVEELGG
HHHHHHCCCHHHCCHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHHCCC
PEVSGVGFGLGVERLLLLLNAETQTTLQSKQLDFYVVGIGDLVQNDVLKVVHELRQMNFV
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC
TEQDYLDRKPKAQFKSADKLNAKYVVTIGESEMNDRIFKLKDMHSGEERTVALSEINTLK
CCHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCCCHHEEECCCCCCCCCEEEHHHHHHHH
DLLK
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA