Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 116492877

GI number: 116492877

Start: 1113418

End: 1115199

Strand: Reverse

Name: aspS

Synonym: PEPE_1115

Alternate gene names: 116492877

Gene position: 1115199-1113418 (Counterclockwise)

Preceding gene: 116492878

Following gene: 116492876

Centisome position: 60.86

GC content: 38.55

Gene sequence:

>1782_bases
ATGAAAAGAACAACATACGCTGGTTTAGTAAATGAACAGTATACTGGCCAAACAGTAACTTTAAAAGGATGGGTTCAAAA
ACGTCGTGATTTGGGACAACTAATTTTTATTGACCTTCGCGATCGTGAAGGGATCGTTCAATTAGTATTTAGTTCAGAAT
TTTCGGCTGATGCATTGAAAATTGCTGATCAATTAAGAAATGAATATGTCCTTGAAATAACTGGGATTGTCAAACAACGT
AAGGAAAAAGACGTTAATCCTAAAATGAAAACGGGACAAATTGAAGTGGAAGTTCAACAAGCTAAGATTTTGAACTCAGC
TAAGACACCTCCATTTGATATTGAGGATGGAGTAAGTGCATCTGATGAATTGAAGATGAAATATCGTTATTTGGATCTTC
GTCGTCCAGAAATGCTTAAAGGTCTTAAAATCCGTAATCGAATTACGCAAGCTGTTCATAGTTATCTTGATGAACAAGGT
TTCTTAGATATTGAAACACCTGACTTAACAAAGTCGACACCCGAAGGAGCTCGTGATTATTTAGTACCTTCTCGGGTTTA
CCCAGGACATTTCTATGCCTTACCTCAATCACCACAGTTGTTTAAACAACTTTTAATGGGAGCTGGAATTGATCGCTACT
ACCAATTAGCACGTTGTTTCCGAGATGAAGATTTACGTGGGGATCGTCAACCTGAATTTACGCAAATCGATTTAGAAACT
AGTTTTTTAACTGCTGAAGAAATTCAAGATATTACGGAAGGCTTAATTGCTCGCGTAATGAAGGATACTTTGGATATTGA
TGTTAAATTACCATTTGATCGAATTTCTTGGAAAGAATCGATGGACCGCTTCGGTACAGATCAACCCGATGTTCGTTTTG
GGATGGAATTAAAAGATATTAGTTCAATTGTGGCTGACTCAGAATTTAAAGTTTTCAGTGGGGCAGTTGCTAACGGCGGA
GTAGTTAAGGCGATTGCTGTTCCTGATGGAGCTAACAACTTATCTAGAAAAGACATTGATAAACTAGGCAAATACGTAGA
GCGCTTTGGTGCTAAGGGATTAGCTTGGTTGAAGATTACAGATGATGGTTTCAGTGGTCCAGTTGCTAAGTTCTTTAATG
CAGAAACTGAAAAACAAATTATGGAACAAACAGGGGCTCAAGTAGGAGACTTACTACTGTTTGCTGCAGATCGTGCCAAG
GTTGTTGGTGATACATTGGGTTATCTCCGTGTTGAACTTGCTAAACGTTTTGATATGATCGATGAGGATCAGTTTGCTTT
CCTTTGGGTAGTAGATTGGCCGTTATTTGACTATGATGAAGGTGACCAACGCTGGGTTGCTGCTCATCATCCATTTACAA
TGCCAAATGAAGAGGATGTTCATTATCTAGATGAAGGCGAAGACCCTCATCAAGCCCATGCCCAAAGTTATGACATCATT
TTAAATGGACTTGAACTAGGCGGTGGTTCAATTCGTATTAACACACGTGAACTTCAAGAAAAGATGCTGAAAGCACTTGG
CTTCAGTCTAGAGAGTGCCAATGACCAATTCGGTTACTTACTAACAGCGCTCGATTATGGTTTCCCACCACATGGCGGTT
TAGCAATTGGTTTAGATCGTTTTGCTAGATTATTAGTTAAACGTGATAATATTCGTGATGTTATTGCATTCCCTAAGAAT
TCTAAGGCATCAGAACCAATGACAGAAGCTCCAACAATGGTTTCAGAAGAACAATTAGCTGAATTATCATTAGATATTGA
TGTTCGTAAACCAGAAGACTAA

Upstream 100 bases:

>100_bases
ATCTTTAAATTAAAGGACATGCATTCTGGAGAAGAACGTACCGTAGCATTAAGCGAAATTAATACATTAAAAGATCTTTT
AAAATAAGCGAGGAAGACTA

Downstream 100 bases:

>100_bases
TAAGAAACTAGAAAAAAGAGGATGGTTAAATCCTCTTTTTTTATTGGATTATATATAAAAAATTATTAAATGAGTATCAG
TCTTCATTTCAAGTGACTGA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKRTTYAGLVNEQYTGQTVTLKGWVQKRRDLGQLIFIDLRDREGIVQLVFSSEFSADALKIADQLRNEYVLEITGIVKQR
KEKDVNPKMKTGQIEVEVQQAKILNSAKTPPFDIEDGVSASDELKMKYRYLDLRRPEMLKGLKIRNRITQAVHSYLDEQG
FLDIETPDLTKSTPEGARDYLVPSRVYPGHFYALPQSPQLFKQLLMGAGIDRYYQLARCFRDEDLRGDRQPEFTQIDLET
SFLTAEEIQDITEGLIARVMKDTLDIDVKLPFDRISWKESMDRFGTDQPDVRFGMELKDISSIVADSEFKVFSGAVANGG
VVKAIAVPDGANNLSRKDIDKLGKYVERFGAKGLAWLKITDDGFSGPVAKFFNAETEKQIMEQTGAQVGDLLLFAADRAK
VVGDTLGYLRVELAKRFDMIDEDQFAFLWVVDWPLFDYDEGDQRWVAAHHPFTMPNEEDVHYLDEGEDPHQAHAQSYDII
LNGLELGGGSIRINTRELQEKMLKALGFSLESANDQFGYLLTALDYGFPPHGGLAIGLDRFARLLVKRDNIRDVIAFPKN
SKASEPMTEAPTMVSEEQLAELSLDIDVRKPED

Sequences:

>Translated_593_residues
MKRTTYAGLVNEQYTGQTVTLKGWVQKRRDLGQLIFIDLRDREGIVQLVFSSEFSADALKIADQLRNEYVLEITGIVKQR
KEKDVNPKMKTGQIEVEVQQAKILNSAKTPPFDIEDGVSASDELKMKYRYLDLRRPEMLKGLKIRNRITQAVHSYLDEQG
FLDIETPDLTKSTPEGARDYLVPSRVYPGHFYALPQSPQLFKQLLMGAGIDRYYQLARCFRDEDLRGDRQPEFTQIDLET
SFLTAEEIQDITEGLIARVMKDTLDIDVKLPFDRISWKESMDRFGTDQPDVRFGMELKDISSIVADSEFKVFSGAVANGG
VVKAIAVPDGANNLSRKDIDKLGKYVERFGAKGLAWLKITDDGFSGPVAKFFNAETEKQIMEQTGAQVGDLLLFAADRAK
VVGDTLGYLRVELAKRFDMIDEDQFAFLWVVDWPLFDYDEGDQRWVAAHHPFTMPNEEDVHYLDEGEDPHQAHAQSYDII
LNGLELGGGSIRINTRELQEKMLKALGFSLESANDQFGYLLTALDYGFPPHGGLAIGLDRFARLLVKRDNIRDVIAFPKN
SKASEPMTEAPTMVSEEQLAELSLDIDVRKPED
>Mature_593_residues
MKRTTYAGLVNEQYTGQTVTLKGWVQKRRDLGQLIFIDLRDREGIVQLVFSSEFSADALKIADQLRNEYVLEITGIVKQR
KEKDVNPKMKTGQIEVEVQQAKILNSAKTPPFDIEDGVSASDELKMKYRYLDLRRPEMLKGLKIRNRITQAVHSYLDEQG
FLDIETPDLTKSTPEGARDYLVPSRVYPGHFYALPQSPQLFKQLLMGAGIDRYYQLARCFRDEDLRGDRQPEFTQIDLET
SFLTAEEIQDITEGLIARVMKDTLDIDVKLPFDRISWKESMDRFGTDQPDVRFGMELKDISSIVADSEFKVFSGAVANGG
VVKAIAVPDGANNLSRKDIDKLGKYVERFGAKGLAWLKITDDGFSGPVAKFFNAETEKQIMEQTGAQVGDLLLFAADRAK
VVGDTLGYLRVELAKRFDMIDEDQFAFLWVVDWPLFDYDEGDQRWVAAHHPFTMPNEEDVHYLDEGEDPHQAHAQSYDII
LNGLELGGGSIRINTRELQEKMLKALGFSLESANDQFGYLLTALDYGFPPHGGLAIGLDRFARLLVKRDNIRDVIAFPKN
SKASEPMTEAPTMVSEEQLAELSLDIDVRKPED

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=601, Percent_Identity=42.2628951747088, Blast_Score=451, Evalue=1e-127,
Organism=Homo sapiens, GI5031815, Length=342, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194272210, Length=362, Percent_Identity=24.585635359116, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI45439306, Length=300, Percent_Identity=23.6666666666667, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI4758762, Length=288, Percent_Identity=25, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI226491502, Length=269, Percent_Identity=23.7918215613383, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788173, Length=597, Percent_Identity=50.251256281407, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1789256, Length=292, Percent_Identity=24.6575342465753, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790571, Length=292, Percent_Identity=23.6301369863014, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=34.5575959933222, Blast_Score=321, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=369, Percent_Identity=22.7642276422764, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=369, Percent_Identity=23.0352303523035, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=312, Percent_Identity=23.3974358974359, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71984122, Length=289, Percent_Identity=24.5674740484429, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=257, Percent_Identity=26.8482490272374, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=622, Percent_Identity=31.3504823151125, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6323011, Length=263, Percent_Identity=26.2357414448669, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=310, Percent_Identity=23.8709677419355, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24584738, Length=607, Percent_Identity=36.0790774299835, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=244, Percent_Identity=27.4590163934426, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640849, Length=294, Percent_Identity=26.530612244898, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640851, Length=294, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921528, Length=243, Percent_Identity=27.1604938271605, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PEDPA (Q03F52)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804612.1
- ProteinModelPortal:   Q03F52
- SMR:   Q03F52
- STRING:   Q03F52
- GeneID:   4417050
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1115
- NMPDR:   fig|278197.10.peg.979
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q03F52
- ProtClustDB:   PRK00476
- BioCyc:   PPEN278197:PEPE_1115-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67137; Mature: 67137

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTTYAGLVNEQYTGQTVTLKGWVQKRRDLGQLIFIDLRDREGIVQLVFSSEFSADALK
CCCCCEECCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHHH
IADQLRNEYVLEITGIVKQRKEKDVNPKMKTGQIEVEVQQAKILNSAKTPPFDIEDGVSA
HHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCC
SDELKMKYRYLDLRRPEMLKGLKIRNRITQAVHSYLDEQGFLDIETPDLTKSTPEGARDY
CHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHC
LVPSRVYPGHFYALPQSPQLFKQLLMGAGIDRYYQLARCFRDEDLRGDRQPEFTQIDLET
CCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCH
SFLTAEEIQDITEGLIARVMKDTLDIDVKLPFDRISWKESMDRFGTDQPDVRFGMELKDI
HHCCHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHCCCCCCCCEECCCHHHH
SSIVADSEFKVFSGAVANGGVVKAIAVPDGANNLSRKDIDKLGKYVERFGAKGLAWLKIT
HHHHCCCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
DDGFSGPVAKFFNAETEKQIMEQTGAQVGDLLLFAADRAKVVGDTLGYLRVELAKRFDMI
CCCCCCCHHHHHCCHHHHHHHHHHCCHHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
DEDQFAFLWVVDWPLFDYDEGDQRWVAAHHPFTMPNEEDVHYLDEGEDPHQAHAQSYDII
CCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCCHHHHHHCCEEEE
LNGLELGGGSIRINTRELQEKMLKALGFSLESANDQFGYLLTALDYGFPPHGGLAIGLDR
EEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECHHH
FARLLVKRDNIRDVIAFPKNSKASEPMTEAPTMVSEEQLAELSLDIDVRKPED
HHHHHHHCCCCCEEEECCCCCCCCCCCHHCCCCCCHHHHHHEEEEEEECCCCC
>Mature Secondary Structure
MKRTTYAGLVNEQYTGQTVTLKGWVQKRRDLGQLIFIDLRDREGIVQLVFSSEFSADALK
CCCCCEECCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHHH
IADQLRNEYVLEITGIVKQRKEKDVNPKMKTGQIEVEVQQAKILNSAKTPPFDIEDGVSA
HHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCC
SDELKMKYRYLDLRRPEMLKGLKIRNRITQAVHSYLDEQGFLDIETPDLTKSTPEGARDY
CHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHC
LVPSRVYPGHFYALPQSPQLFKQLLMGAGIDRYYQLARCFRDEDLRGDRQPEFTQIDLET
CCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCH
SFLTAEEIQDITEGLIARVMKDTLDIDVKLPFDRISWKESMDRFGTDQPDVRFGMELKDI
HHCCHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHCCCCCCCCEECCCHHHH
SSIVADSEFKVFSGAVANGGVVKAIAVPDGANNLSRKDIDKLGKYVERFGAKGLAWLKIT
HHHHCCCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
DDGFSGPVAKFFNAETEKQIMEQTGAQVGDLLLFAADRAKVVGDTLGYLRVELAKRFDMI
CCCCCCCHHHHHCCHHHHHHHHHHCCHHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
DEDQFAFLWVVDWPLFDYDEGDQRWVAAHHPFTMPNEEDVHYLDEGEDPHQAHAQSYDII
CCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCCHHHHHHCCEEEE
LNGLELGGGSIRINTRELQEKMLKALGFSLESANDQFGYLLTALDYGFPPHGGLAIGLDR
EEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECHHH
FARLLVKRDNIRDVIAFPKNSKASEPMTEAPTMVSEEQLAELSLDIDVRKPED
HHHHHHHCCCCCEEEECCCCCCCCCCCHHCCCCCCHHHHHHEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA