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The map label for this gene is rnhB

Identifier: 116492734

GI number: 116492734

Start: 976640

End: 977407

Strand: Reverse

Name: rnhB

Synonym: PEPE_0971

Alternate gene names: 116492734

Gene position: 977407-976640 (Counterclockwise)

Preceding gene: 116492735

Following gene: 116492733

Centisome position: 53.34

GC content: 38.8

Gene sequence:

>768_bases
TTGGCGACGATTAAGGAAATTAAGACGCAGCTTTTAAGTGTTTATACACTAGATGACCCGTTATTAGTTGAACTTTCAGA
AGATCCACGAACTGGTGTTCAACGCTTAATTAAACATAAACAAAAGGAAATTAACCAATTAAGATTAAAAGAAGAAGCAT
TTCAGGCACGATTTACGTTAGAACGAGAATTATGGTCAAATGGAATTGACTTAGTAGCCGGGATTGATGAAGTGGGTCGG
GGATGTTTAGCGGGTCCGGTCGTCACTGCCAGTGTAGTCTTGGATGAAACTTTCGATTTAATCGACGTTAATGACTCTAA
GCAGCTCAGTAGTGAATTGCGAGAGGAATTATATGCCAAAATCCTTACAGAGGCTGTGAGCGTCGGTATTGGCGTCTGTT
CTAACCAAAAAATTGATGAAGTTAATATTTTAAATGCTACAAAACTTGCGATGCGAGAAGCAGTGGGTAACTTAAATGTT
ACTCCACAACATTTGTTGATTGATGCAGTCAATGCGCCGATAGAAATCCCAAAAACTGTGATGTTTAAGGGTGATTCTAA
AAGTATCAGTATTGCAGCCGCTTCAATTGTTGCTAAGGTATATCGTGACCATTTAATGCGTTCTTATGCTGCACTCTATC
CAGGCTACGGATTTGATAAAAACGTGGGTTATGGTACTAAGCAACATATTGAGGGGTTAAAAACTTATGGAGTAACCCCC
ATCCATCGTCAGACTTTTGAACCGGTACCTGAATTTTTAATAAAATAA

Upstream 100 bases:

>100_bases
CGTGACGACTACGATCGTGCTAGTCAGCGAATCTTGTTAGACGCACGTAAAGGAAAACTGGGTCGTTTCACTTTAGACTT
TGTAGAGGAGTTTGAAGACC

Downstream 100 bases:

>100_bases
ATTAACCTAAAAACACGTAAAGTACATTTGAGGTGATCAAATGGAAATACGTGATTTTTTGATTAGAGTAAAAGTGGGGA
CGAATTTAGGCATTAAAAAG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MATIKEIKTQLLSVYTLDDPLLVELSEDPRTGVQRLIKHKQKEINQLRLKEEAFQARFTLERELWSNGIDLVAGIDEVGR
GCLAGPVVTASVVLDETFDLIDVNDSKQLSSELREELYAKILTEAVSVGIGVCSNQKIDEVNILNATKLAMREAVGNLNV
TPQHLLIDAVNAPIEIPKTVMFKGDSKSISIAAASIVAKVYRDHLMRSYAALYPGYGFDKNVGYGTKQHIEGLKTYGVTP
IHRQTFEPVPEFLIK

Sequences:

>Translated_255_residues
MATIKEIKTQLLSVYTLDDPLLVELSEDPRTGVQRLIKHKQKEINQLRLKEEAFQARFTLERELWSNGIDLVAGIDEVGR
GCLAGPVVTASVVLDETFDLIDVNDSKQLSSELREELYAKILTEAVSVGIGVCSNQKIDEVNILNATKLAMREAVGNLNV
TPQHLLIDAVNAPIEIPKTVMFKGDSKSISIAAASIVAKVYRDHLMRSYAALYPGYGFDKNVGYGTKQHIEGLKTYGVTP
IHRQTFEPVPEFLIK
>Mature_254_residues
ATIKEIKTQLLSVYTLDDPLLVELSEDPRTGVQRLIKHKQKEINQLRLKEEAFQARFTLERELWSNGIDLVAGIDEVGRG
CLAGPVVTASVVLDETFDLIDVNDSKQLSSELREELYAKILTEAVSVGIGVCSNQKIDEVNILNATKLAMREAVGNLNVT
PQHLLIDAVNAPIEIPKTVMFKGDSKSISIAAASIVAKVYRDHLMRSYAALYPGYGFDKNVGYGTKQHIEGLKTYGVTPI
HRQTFEPVPEFLIK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=46.6666666666667, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PEDPA (Q03FJ5)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804469.1
- ProteinModelPortal:   Q03FJ5
- SMR:   Q03FJ5
- STRING:   Q03FJ5
- GeneID:   4417783
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0971
- NMPDR:   fig|278197.10.peg.861
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q03FJ5
- BioCyc:   PPEN278197:PEPE_0971-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28280; Mature: 28149

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKEIKTQLLSVYTLDDPLLVELSEDPRTGVQRLIKHKQKEINQLRLKEEAFQARFTL
CCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERELWSNGIDLVAGIDEVGRGCLAGPVVTASVVLDETFDLIDVNDSKQLSSELREELYAK
HHHHHCCCCCEEECHHHHCCCHHCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHH
ILTEAVSVGIGVCSNQKIDEVNILNATKLAMREAVGNLNVTPQHLLIDAVNAPIEIPKTV
HHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEE
MFKGDSKSISIAAASIVAKVYRDHLMRSYAALYPGYGFDKNVGYGTKQHIEGLKTYGVTP
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCC
IHRQTFEPVPEFLIK
CHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATIKEIKTQLLSVYTLDDPLLVELSEDPRTGVQRLIKHKQKEINQLRLKEEAFQARFTL
CCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERELWSNGIDLVAGIDEVGRGCLAGPVVTASVVLDETFDLIDVNDSKQLSSELREELYAK
HHHHHCCCCCEEECHHHHCCCHHCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHH
ILTEAVSVGIGVCSNQKIDEVNILNATKLAMREAVGNLNVTPQHLLIDAVNAPIEIPKTV
HHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEE
MFKGDSKSISIAAASIVAKVYRDHLMRSYAALYPGYGFDKNVGYGTKQHIEGLKTYGVTP
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCC
IHRQTFEPVPEFLIK
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA