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The map label for this gene is tpiA

Identifier: 116492263

GI number: 116492263

Start: 514624

End: 515379

Strand: Direct

Name: tpiA

Synonym: PEPE_0461

Alternate gene names: 116492263

Gene position: 514624-515379 (Clockwise)

Preceding gene: 116492262

Following gene: 116492264

Centisome position: 28.08

GC content: 40.21

Gene sequence:

>756_bases
TTGCGTACTCCAATTATTGCTGGAAACTGGAAAATGAACAAAAACCCCCAAGAAACTCAAGAATTCTTGGATGCTATCAA
AGGTAAGTTACCTGATGCTTCTGTAGTAGAATCAGTTATTGCTGCTCCTGCTATCGATTTAAGCACTTTAGTAGCTTTCT
CAAAAGATGAACAACTAAAGACTGCTGCTGAAAACAGCTACTTCGAAGACGAAGGTGCTTTCACTGGCGAAACAAGTCCT
AAGGCTTTAAACGAAATGGGCGTTAACTATGTTGTTATCGGTCATTCAGAACGTCGACAATACTTCGGTGAAACTGACGA
AGATATTAACAAGAAGGCTAAGGCAATCTTCAACAACAACATGACACCAATCATTTGCTGTGGAGAAACTCTTGAACAAC
GTGAAGCTGGCGAAACTAATGAATGGGTTGCTGGTCAAATCACAAACGCTCTTAAAGATTTAACTGCAGAACAAGTTGCT
GCATCAGTGATCGCTTACGAACCAATCTGGGCTATCGGTACTGGTAAGACAGCTTCAAGTGATCAAGCTCAAGAAGTTTG
TCACGTAATTCGTGAAACAGTTGCTAAGCTTTACGATCAAACTGTTGCTGACAAAGTACGTATTCAATACGGTGGTAGTG
TAAAACCTGCTAACATTGCTGAATTAATGGGCAAAGAAGATATTGATGGTGGACTTGTTGGTGGAGCATCACTTGATCCA
GAATCATTCCTAGAATTAGTTAACTACAAGGGTTAA

Upstream 100 bases:

>100_bases
GCTGCTATTTCAAACAAATAATTAAAGTTGTAGAAATCGAAGAATCAGTTGATTCTTCTTTTCTGCTACATAACCTGTAA
TTCGGAAAGGATGTTTTATT

Downstream 100 bases:

>100_bases
TATAATTAAGGAAATATAATATAATAATAGTTGAACTGCGTTAAGCAGATTTAAAAATGTGAGGAGATATTTAAAATGTC
ACTTATTACTGATATTTACG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRTPIIAGNWKMNKNPQETQEFLDAIKGKLPDASVVESVIAAPAIDLSTLVAFSKDEQLKTAAENSYFEDEGAFTGETSP
KALNEMGVNYVVIGHSERRQYFGETDEDINKKAKAIFNNNMTPIICCGETLEQREAGETNEWVAGQITNALKDLTAEQVA
ASVIAYEPIWAIGTGKTASSDQAQEVCHVIRETVAKLYDQTVADKVRIQYGGSVKPANIAELMGKEDIDGGLVGGASLDP
ESFLELVNYKG

Sequences:

>Translated_251_residues
MRTPIIAGNWKMNKNPQETQEFLDAIKGKLPDASVVESVIAAPAIDLSTLVAFSKDEQLKTAAENSYFEDEGAFTGETSP
KALNEMGVNYVVIGHSERRQYFGETDEDINKKAKAIFNNNMTPIICCGETLEQREAGETNEWVAGQITNALKDLTAEQVA
ASVIAYEPIWAIGTGKTASSDQAQEVCHVIRETVAKLYDQTVADKVRIQYGGSVKPANIAELMGKEDIDGGLVGGASLDP
ESFLELVNYKG
>Mature_251_residues
MRTPIIAGNWKMNKNPQETQEFLDAIKGKLPDASVVESVIAAPAIDLSTLVAFSKDEQLKTAAENSYFEDEGAFTGETSP
KALNEMGVNYVVIGHSERRQYFGETDEDINKKAKAIFNNNMTPIICCGETLEQREAGETNEWVAGQITNALKDLTAEQVA
ASVIAYEPIWAIGTGKTASSDQAQEVCHVIRETVAKLYDQTVADKVRIQYGGSVKPANIAELMGKEDIDGGLVGGASLDP
ESFLELVNYKG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=41.501976284585, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=41.501976284585, Blast_Score=174, Evalue=8e-44,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=42.914979757085, Blast_Score=201, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17536593, Length=256, Percent_Identity=42.96875, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=252, Percent_Identity=39.2857142857143, Blast_Score=175, Evalue=5e-45,
Organism=Drosophila melanogaster, GI28572008, Length=249, Percent_Identity=44.578313253012, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI28572006, Length=249, Percent_Identity=44.578313253012, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=44.578313253012, Blast_Score=191, Evalue=4e-49,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PEDPA (Q03GW6)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803998.1
- ProteinModelPortal:   Q03GW6
- SMR:   Q03GW6
- STRING:   Q03GW6
- GeneID:   4418358
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0461
- NMPDR:   fig|278197.10.peg.429
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q03GW6
- ProtClustDB:   PRK00042
- BioCyc:   PPEN278197:PEPE_0461-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27225; Mature: 27225

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPIIAGNWKMNKNPQETQEFLDAIKGKLPDASVVESVIAAPAIDLSTLVAFSKDEQLK
CCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHH
TAAENSYFEDEGAFTGETSPKALNEMGVNYVVIGHSERRQYFGETDEDINKKAKAIFNNN
HHHHCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHCCCCCHHHHHHHHHHHCCC
MTPIICCGETLEQREAGETNEWVAGQITNALKDLTAEQVAASVIAYEPIWAIGTGKTASS
CCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
DQAQEVCHVIRETVAKLYDQTVADKVRIQYGGSVKPANIAELMGKEDIDGGLVGGASLDP
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCCCCCCCEECCCCCCH
ESFLELVNYKG
HHHHHHHHCCC
>Mature Secondary Structure
MRTPIIAGNWKMNKNPQETQEFLDAIKGKLPDASVVESVIAAPAIDLSTLVAFSKDEQLK
CCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHH
TAAENSYFEDEGAFTGETSPKALNEMGVNYVVIGHSERRQYFGETDEDINKKAKAIFNNN
HHHHCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHCCCCCHHHHHHHHHHHCCC
MTPIICCGETLEQREAGETNEWVAGQITNALKDLTAEQVAASVIAYEPIWAIGTGKTASS
CCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
DQAQEVCHVIRETVAKLYDQTVADKVRIQYGGSVKPANIAELMGKEDIDGGLVGGASLDP
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCCCCCCCEECCCCCCH
ESFLELVNYKG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA