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The map label for this gene is pgk

Identifier: 116492262

GI number: 116492262

Start: 513342

End: 514544

Strand: Direct

Name: pgk

Synonym: PEPE_0460

Alternate gene names: 116492262

Gene position: 513342-514544 (Clockwise)

Preceding gene: 116492261

Following gene: 116492263

Centisome position: 28.01

GC content: 41.15

Gene sequence:

>1203_bases
TTGGCTAAATTAACTGTTTCAGATTTAGATGTTAAGAACAAGAAAGTTTTGATGCGCGTTGACTTTAACGTGCCAGTTAA
AAATGGTGTGATTGGTGATGACAACCGTGTCGTTGCTGCTCTTCCAACAATTAAATACATTATCGAAAACGGTGGAAAGG
CAATTCTTTTCTCTCACTTAGGTCGTATCAAGACTGAAGAAGACAAAAAAGAATTGACTCTTCGTCCAGTTGCTCAACGC
CTTTCAGATCTTCTTGGCAAACCAGTTACATTTGTTCCTGTAACACGCGGTGCACAACTTGAAGATGCTGTTAATGGCAT
GAACGACGGCGATGTATTATTGTTCGAAAACACACGTTTTGAAGACTTAGATGGCAAGAAGGAAAGTAAAAACGATCCTG
AACTTGGCAAATACTGGGCATCACTTGGCGACATGTTCGTAAACGATGCTTTCGGTACAGCTCACCGTGCCCACGCTTCA
AACGTTGGTGTTGCTGAAGCAATGAAGGCTGACGGCAAGCAAGTTGCTGCTGGTTTCCTTATGGAAAAAGAAATCAAGTT
CTTGGGTGAAGCTGTTGACGAACCTAAGCATCCATTTGTTGCTATTTTAGGTGGCGCAAAGGTTTCTGACAAGATTGGTG
TAATTGATCACTTACTTAATAAAGCTGACAAAGTTATTATTGGTGGTGGTATGACATACACATTCTACGCTGCTAAAGGC
ATGAGCATTGGTAACTCACTTGTTGAAGCTGACAAGATTGATGTTGCTAAAGAAATCCTTGCTAAGGGTGGCGACAAGAT
TGTTCTTCCTTCAGACAGTATCGTTGCTGAAAAATTTGATAACGATGTTCCTAACAAAGTTGTCGAAGGCGATATTCCTG
ATGGTTACATGGCTCTTGATATTGGACCTAAGTCAGTTGCAGAATTTAAGGATGTCCTTAAAGATGCTAAGACTGTTGTA
TGGAACGGACCAATGGGTGTATTCGAAATGAGCAACTATGCTAACGGTACACTTGAAGTTGGTAAGTTCTTAGGTACATT
ATCAGACGCTACAACAATTGTTGGTGGTGGTGACTCAACTGCTGCTGTTAAGCAATTAGGCGTTGGCGACCAATTGACAC
ATATCTCAACTGGTGGTGGAGCTTCACTTGAATACCTTGAAGGTAAAGAACTTCCTGGTATCGCTGCTATTTCAAACAAA
TAA

Upstream 100 bases:

>100_bases
ATTTCAAACCGTCTAATCGGTCTAGGGACGGAGGGAGGTCACTTCCTCCGTCCCTTTTTTTAGAAAAAATAATTTATTAA
TGATTTAGGAGGAAATTCTC

Downstream 100 bases:

>100_bases
TTAAAGTTGTAGAAATCGAAGAATCAGTTGATTCTTCTTTTCTGCTACATAACCTGTAATTCGGAAAGGATGTTTTATTT
TGCGTACTCCAATTATTGCT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKLTVSDLDVKNKKVLMRVDFNVPVKNGVIGDDNRVVAALPTIKYIIENGGKAILFSHLGRIKTEEDKKELTLRPVAQR
LSDLLGKPVTFVPVTRGAQLEDAVNGMNDGDVLLFENTRFEDLDGKKESKNDPELGKYWASLGDMFVNDAFGTAHRAHAS
NVGVAEAMKADGKQVAAGFLMEKEIKFLGEAVDEPKHPFVAILGGAKVSDKIGVIDHLLNKADKVIIGGGMTYTFYAAKG
MSIGNSLVEADKIDVAKEILAKGGDKIVLPSDSIVAEKFDNDVPNKVVEGDIPDGYMALDIGPKSVAEFKDVLKDAKTVV
WNGPMGVFEMSNYANGTLEVGKFLGTLSDATTIVGGGDSTAAVKQLGVGDQLTHISTGGGASLEYLEGKELPGIAAISNK

Sequences:

>Translated_400_residues
MAKLTVSDLDVKNKKVLMRVDFNVPVKNGVIGDDNRVVAALPTIKYIIENGGKAILFSHLGRIKTEEDKKELTLRPVAQR
LSDLLGKPVTFVPVTRGAQLEDAVNGMNDGDVLLFENTRFEDLDGKKESKNDPELGKYWASLGDMFVNDAFGTAHRAHAS
NVGVAEAMKADGKQVAAGFLMEKEIKFLGEAVDEPKHPFVAILGGAKVSDKIGVIDHLLNKADKVIIGGGMTYTFYAAKG
MSIGNSLVEADKIDVAKEILAKGGDKIVLPSDSIVAEKFDNDVPNKVVEGDIPDGYMALDIGPKSVAEFKDVLKDAKTVV
WNGPMGVFEMSNYANGTLEVGKFLGTLSDATTIVGGGDSTAAVKQLGVGDQLTHISTGGGASLEYLEGKELPGIAAISNK
>Mature_399_residues
AKLTVSDLDVKNKKVLMRVDFNVPVKNGVIGDDNRVVAALPTIKYIIENGGKAILFSHLGRIKTEEDKKELTLRPVAQRL
SDLLGKPVTFVPVTRGAQLEDAVNGMNDGDVLLFENTRFEDLDGKKESKNDPELGKYWASLGDMFVNDAFGTAHRAHASN
VGVAEAMKADGKQVAAGFLMEKEIKFLGEAVDEPKHPFVAILGGAKVSDKIGVIDHLLNKADKVIIGGGMTYTFYAAKGM
SIGNSLVEADKIDVAKEILAKGGDKIVLPSDSIVAEKFDNDVPNKVVEGDIPDGYMALDIGPKSVAEFKDVLKDAKTVVW
NGPMGVFEMSNYANGTLEVGKFLGTLSDATTIVGGGDSTAAVKQLGVGDQLTHISTGGGASLEYLEGKELPGIAAISNK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=419, Percent_Identity=44.3914081145585, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI31543397, Length=417, Percent_Identity=43.8848920863309, Blast_Score=317, Evalue=1e-86,
Organism=Escherichia coli, GI1789294, Length=403, Percent_Identity=42.1836228287841, Blast_Score=297, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=420, Percent_Identity=45.2380952380952, Blast_Score=330, Evalue=6e-91,
Organism=Saccharomyces cerevisiae, GI6319857, Length=421, Percent_Identity=46.0807600950119, Blast_Score=332, Evalue=5e-92,
Organism=Drosophila melanogaster, GI17136394, Length=418, Percent_Identity=44.0191387559809, Blast_Score=310, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24581203, Length=420, Percent_Identity=43.5714285714286, Blast_Score=305, Evalue=4e-83,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_PEDPA (Q03GW7)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803997.1
- STRING:   Q03GW7
- GeneID:   4418357
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0460
- NMPDR:   fig|278197.10.peg.428
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q03GW7
- ProtClustDB:   PRK00073
- BioCyc:   PPEN278197:PEPE_0460-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42548; Mature: 42417

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 119-119 BINDING 156-156 BINDING 211-211 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTVSDLDVKNKKVLMRVDFNVPVKNGVIGDDNRVVAALPTIKYIIENGGKAILFSHL
CCEEEECCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEECHHHHHHHHCCCCEEEEHHH
GRIKTEEDKKELTLRPVAQRLSDLLGKPVTFVPVTRGAQLEDAVNGMNDGDVLLFENTRF
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCEEEEECCCC
EDLDGKKESKNDPELGKYWASLGDMFVNDAFGTAHRAHASNVGVAEAMKADGKQVAAGFL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHH
MEKEIKFLGEAVDEPKHPFVAILGGAKVSDKIGVIDHLLNKADKVIIGGGMTYTFYAAKG
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEEEECC
MSIGNSLVEADKIDVAKEILAKGGDKIVLPSDSIVAEKFDNDVPNKVVEGDIPDGYMALD
CCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEEE
IGPKSVAEFKDVLKDAKTVVWNGPMGVFEMSNYANGTLEVGKFLGTLSDATTIVGGGDST
CCHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCH
AAVKQLGVGDQLTHISTGGGASLEYLEGKELPGIAAISNK
HHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCEEECCCC
>Mature Secondary Structure 
AKLTVSDLDVKNKKVLMRVDFNVPVKNGVIGDDNRVVAALPTIKYIIENGGKAILFSHL
CEEEECCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEECHHHHHHHHCCCCEEEEHHH
GRIKTEEDKKELTLRPVAQRLSDLLGKPVTFVPVTRGAQLEDAVNGMNDGDVLLFENTRF
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCEEEEECCCC
EDLDGKKESKNDPELGKYWASLGDMFVNDAFGTAHRAHASNVGVAEAMKADGKQVAAGFL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHH
MEKEIKFLGEAVDEPKHPFVAILGGAKVSDKIGVIDHLLNKADKVIIGGGMTYTFYAAKG
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEEEECC
MSIGNSLVEADKIDVAKEILAKGGDKIVLPSDSIVAEKFDNDVPNKVVEGDIPDGYMALD
CCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEEE
IGPKSVAEFKDVLKDAKTVVWNGPMGVFEMSNYANGTLEVGKFLGTLSDATTIVGGGDST
CCHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCH
AAVKQLGVGDQLTHISTGGGASLEYLEGKELPGIAAISNK
HHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA