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The map label for this gene is ansA [H]

Identifier: 116492105

GI number: 116492105

Start: 345478

End: 346449

Strand: Direct

Name: ansA [H]

Synonym: PEPE_0301

Alternate gene names: 116492105

Gene position: 345478-346449 (Clockwise)

Preceding gene: 116492103

Following gene: 116492106

Centisome position: 18.85

GC content: 38.99

Gene sequence:

>972_bases
ATGAAGAAAATACTAGCATTACATACAGGTGGAACGATTTCGATGTCTGCCAGCAATGATACTGTTAAAACTAATGAACA
AAACCCATTACTAGATAGCCAATTTATCAACAAAAATATTGACCTAGTTAATGAAGTGATTTTGAATAAACCTTCAGAAC
ACATTACGCCAGATGATATGTTAATTATTAAAAAAAGGGTCAAAAAGGCACTAGATAACAATGAGTGTGATGGCATTGTA
ATCACGCACGGAACGGATACCTTGGAAGAAACTGCGTACTTTTTAGATTTAACGATCGAAAATAAAATTCCAGTTGTGCT
AACGGGGGCCATGCGCTCTTCCAACGAACTAGGTTCGGATGGTGTGTATAATTTCCAAAGTGCAATTCTGACGGCTGCTT
CTGATGAAGCCAGTGATAAAGGGGTTTTAGTGGTCATGAATGATGAGATCCATACTGCCCGTTTTGTTACGAAAACCCAT
ACTACTAATGTAGATACGTTTAGAACCCCAACTTTTGGGCCGCTCGGAATTGTTTATAAACGGGATATCCGATTCTTTCA
AGCTTTAGTTAATCAGCATGTTTGTGATATTGACCACCTTGAAAAAGGAGTCTATTTAATTAAAGCTTACGCAGGAATCC
CTAAAGAAATATTTGAAATTATTAATCGCCCAGGGACCAAGGGGATTGTAATTGAAGGGCTAGGAGCTGGAAATCTACCA
GTAGAAGTAGTCGAGCCCATTCAAGCATTAATTGATAATGATATTACGGTTGTAATGGTTTCACGTTGTTACAATGGCTT
GGCCGAACCCATCTACGATTATGTGGGCGGCGGAATTGATTTAGAGAGAATGGGCGTTGTATTCTGCCAAGGTCTCAATG
GTCAAAAAGCACGTATTAAGTTACAAGTGGCAGTAAGTGCTGGTATGAATTCCAAAGATATTGCTAAGTATATGCATGAC
GCGGTGTCATAG

Upstream 100 bases:

>100_bases
CCTCTGAATTAATAGAATTATTTTAACGCATTTTGAGGAGCTGTAAATAGCCAAGAAAATGATATGATATGAATAACTAA
TAATAAAGAGGAATTTGATA

Downstream 100 bases:

>100_bases
AAGTAGATAAGAGTATAATACAGAGTTAATAAATTTTGAAAGTGAGGAATTAGAATGCCAAGTTCAATTAAAGACGATTT
TTATGATGCAGTGAATGGTG

Product: asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKKILALHTGGTISMSASNDTVKTNEQNPLLDSQFINKNIDLVNEVILNKPSEHITPDDMLIIKKRVKKALDNNECDGIV
ITHGTDTLEETAYFLDLTIENKIPVVLTGAMRSSNELGSDGVYNFQSAILTAASDEASDKGVLVVMNDEIHTARFVTKTH
TTNVDTFRTPTFGPLGIVYKRDIRFFQALVNQHVCDIDHLEKGVYLIKAYAGIPKEIFEIINRPGTKGIVIEGLGAGNLP
VEVVEPIQALIDNDITVVMVSRCYNGLAEPIYDYVGGGIDLERMGVVFCQGLNGQKARIKLQVAVSAGMNSKDIAKYMHD
AVS

Sequences:

>Translated_323_residues
MKKILALHTGGTISMSASNDTVKTNEQNPLLDSQFINKNIDLVNEVILNKPSEHITPDDMLIIKKRVKKALDNNECDGIV
ITHGTDTLEETAYFLDLTIENKIPVVLTGAMRSSNELGSDGVYNFQSAILTAASDEASDKGVLVVMNDEIHTARFVTKTH
TTNVDTFRTPTFGPLGIVYKRDIRFFQALVNQHVCDIDHLEKGVYLIKAYAGIPKEIFEIINRPGTKGIVIEGLGAGNLP
VEVVEPIQALIDNDITVVMVSRCYNGLAEPIYDYVGGGIDLERMGVVFCQGLNGQKARIKLQVAVSAGMNSKDIAKYMHD
AVS
>Mature_323_residues
MKKILALHTGGTISMSASNDTVKTNEQNPLLDSQFINKNIDLVNEVILNKPSEHITPDDMLIIKKRVKKALDNNECDGIV
ITHGTDTLEETAYFLDLTIENKIPVVLTGAMRSSNELGSDGVYNFQSAILTAASDEASDKGVLVVMNDEIHTARFVTKTH
TTNVDTFRTPTFGPLGIVYKRDIRFFQALVNQHVCDIDHLEKGVYLIKAYAGIPKEIFEIINRPGTKGIVIEGLGAGNLP
VEVVEPIQALIDNDITVVMVSRCYNGLAEPIYDYVGGGIDLERMGVVFCQGLNGQKARIKLQVAVSAGMNSKDIAKYMHD
AVS

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=299, Percent_Identity=23.7458193979933, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1789327, Length=279, Percent_Identity=35.4838709677419, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788065, Length=347, Percent_Identity=27.9538904899135, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558280, Length=338, Percent_Identity=26.3313609467456, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558278, Length=338, Percent_Identity=26.3313609467456, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=338, Percent_Identity=26.0355029585799, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207374, Length=338, Percent_Identity=26.3313609467456, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320527, Length=329, Percent_Identity=31.0030395136778, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323189, Length=325, Percent_Identity=29.5384615384615, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323187, Length=325, Percent_Identity=29.5384615384615, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323186, Length=325, Percent_Identity=29.5384615384615, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323184, Length=325, Percent_Identity=29.5384615384615, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24645530, Length=249, Percent_Identity=31.3253012048193, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639609, Length=275, Percent_Identity=26.1818181818182, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35365; Mature: 35365

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILALHTGGTISMSASNDTVKTNEQNPLLDSQFINKNIDLVNEVILNKPSEHITPDDM
CCCEEEEECCCEEEEECCCCCEECCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCHH
LIIKKRVKKALDNNECDGIVITHGTDTLEETAYFLDLTIENKIPVVLTGAMRSSNELGSD
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHCEEEEEEEECCCCCEEEECCCCCCCCCCCC
GVYNFQSAILTAASDEASDKGVLVVMNDEIHTARFVTKTHTTNVDTFRTPTFGPLGIVYK
CCHHHHHHHEEECCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEH
RDIRFFQALVNQHVCDIDHLEKGVYLIKAYAGIPKEIFEIINRPGTKGIVIEGLGAGNLP
HHHHHHHHHHHCCCCCHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCCEEEEECCCCCCC
VEVVEPIQALIDNDITVVMVSRCYNGLAEPIYDYVGGGIDLERMGVVFCQGLNGQKARIK
HHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCCCCCEEEE
LQVAVSAGMNSKDIAKYMHDAVS
EEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKILALHTGGTISMSASNDTVKTNEQNPLLDSQFINKNIDLVNEVILNKPSEHITPDDM
CCCEEEEECCCEEEEECCCCCEECCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCHH
LIIKKRVKKALDNNECDGIVITHGTDTLEETAYFLDLTIENKIPVVLTGAMRSSNELGSD
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHCEEEEEEEECCCCCEEEECCCCCCCCCCCC
GVYNFQSAILTAASDEASDKGVLVVMNDEIHTARFVTKTHTTNVDTFRTPTFGPLGIVYK
CCHHHHHHHEEECCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEH
RDIRFFQALVNQHVCDIDHLEKGVYLIKAYAGIPKEIFEIINRPGTKGIVIEGLGAGNLP
HHHHHHHHHHHCCCCCHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCCEEEEECCCCCCC
VEVVEPIQALIDNDITVVMVSRCYNGLAEPIYDYVGGGIDLERMGVVFCQGLNGQKARIK
HHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCCCCCEEEE
LQVAVSAGMNSKDIAKYMHDAVS
EEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]