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The map label for this gene is ipk

Identifier: 116492075

GI number: 116492075

Start: 317481

End: 318332

Strand: Direct

Name: ipk

Synonym: PEPE_0271

Alternate gene names: 116492075

Gene position: 317481-318332 (Clockwise)

Preceding gene: 116492074

Following gene: 116492076

Centisome position: 17.33

GC content: 38.26

Gene sequence:

>852_bases
ATGGAAATCACCGAGAAAGCTCCAGCCAAGTTAAACCTATTTTTGGATACGCCGTTTAATCATCCTGATGGATTACCAGA
ATGGAACATGATTATGACCTCGGTCGATTTAGCGGATTATGTAAAAATCGAAAGTATTCCTGGTAAAATGGGAATTAAAG
TTCGAACGAATACCGGTTTTTTACCTAACGATAGTCGTAATTTGGCGTATCAAGCTGCCCGGATTTTACAAGAAAAATAT
CACGTACGTGCCAAAGTAATTATAGAAATACGTAAACACATTCCGGTTGCAGCTGGATTAGGCGGAGGTTCGTCTGATGC
TGCTGCCGTTTTACGAGGATTAAATAAACTATGGAATCTTGAGCTTAGCTTAGAAGTATTAGCTAAAATTGGTTTAGAAA
TTGATTCCGATGTACCATTTTGTGTATATAGTCGCACGGCACATGTTTTGGGAAAAGGTGAAAAAGTAATTCCTTTAAAA
AAATTACCTCCTATGTGGGTGGTGATTGCCAAACCGAAACTTAGTGTGTCGACGCCGTATATCCTTAGTAAAATCGAACA
CAAAGAGCTCCAACATTCTAATATTGAATCAATTTTAGATGCAATTGAGCGTGAAGATTATACAGACATTTGTCGTTATA
TGGGCAATGTTTTAGAGCAAGTAACCGGAAAAGAACATCCGGAAGTTTTAAAAATTAAAAATCGAATTAAACAATACGGG
GCAGATGCAGCTCAAATGAGTGGGACTGGACCAACGGTGTTTGGAGTTGCGTCAAAATCAAGCCGAGCCAAGCACATCGT
AAATAGCTTGAAGGGCTTTTGTCATGAAGTATATTTAGTTCGAATTTTGTGA

Upstream 100 bases:

>100_bases
GATAGTTGGGGCATTTAAGCTTTTATGACTATTAGCCTTTTTTATTTTTACCATTTTTTAGTATAATTTAGGTTAAAAGC
AAACGAAACAGGTGAAGTAG

Downstream 100 bases:

>100_bases
GGTATAATAATGGCTAATCATGAAGCAACAAAACAGAAGATCATCGATGCACTATTTGAGTTGTTAAAAGATAATGACTT
AGCCAGTATATCGGTAAGTA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MEITEKAPAKLNLFLDTPFNHPDGLPEWNMIMTSVDLADYVKIESIPGKMGIKVRTNTGFLPNDSRNLAYQAARILQEKY
HVRAKVIIEIRKHIPVAAGLGGGSSDAAAVLRGLNKLWNLELSLEVLAKIGLEIDSDVPFCVYSRTAHVLGKGEKVIPLK
KLPPMWVVIAKPKLSVSTPYILSKIEHKELQHSNIESILDAIEREDYTDICRYMGNVLEQVTGKEHPEVLKIKNRIKQYG
ADAAQMSGTGPTVFGVASKSSRAKHIVNSLKGFCHEVYLVRIL

Sequences:

>Translated_283_residues
MEITEKAPAKLNLFLDTPFNHPDGLPEWNMIMTSVDLADYVKIESIPGKMGIKVRTNTGFLPNDSRNLAYQAARILQEKY
HVRAKVIIEIRKHIPVAAGLGGGSSDAAAVLRGLNKLWNLELSLEVLAKIGLEIDSDVPFCVYSRTAHVLGKGEKVIPLK
KLPPMWVVIAKPKLSVSTPYILSKIEHKELQHSNIESILDAIEREDYTDICRYMGNVLEQVTGKEHPEVLKIKNRIKQYG
ADAAQMSGTGPTVFGVASKSSRAKHIVNSLKGFCHEVYLVRIL
>Mature_283_residues
MEITEKAPAKLNLFLDTPFNHPDGLPEWNMIMTSVDLADYVKIESIPGKMGIKVRTNTGFLPNDSRNLAYQAARILQEKY
HVRAKVIIEIRKHIPVAAGLGGGSSDAAAVLRGLNKLWNLELSLEVLAKIGLEIDSDVPFCVYSRTAHVLGKGEKVIPLK
KLPPMWVVIAKPKLSVSTPYILSKIEHKELQHSNIESILDAIEREDYTDICRYMGNVLEQVTGKEHPEVLKIKNRIKQYG
ADAAQMSGTGPTVFGVASKSSRAKHIVNSLKGFCHEVYLVRIL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=283, Percent_Identity=29.3286219081272, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PEDPA (Q03HF4)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803810.1
- ProteinModelPortal:   Q03HF4
- SMR:   Q03HF4
- STRING:   Q03HF4
- GeneID:   4418548
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0271
- NMPDR:   fig|278197.10.peg.247
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- PhylomeDB:   Q03HF4
- ProtClustDB:   PRK00128
- BioCyc:   PPEN278197:PEPE_0271-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31432; Mature: 31432

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITEKAPAKLNLFLDTPFNHPDGLPEWNMIMTSVDLADYVKIESIPGKMGIKVRTNTGF
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHEECCCHHHHEEEECCCCCCCEEEECCCCC
LPNDSRNLAYQAARILQEKYHVRAKVIIEIRKHIPVAAGLGGGSSDAAAVLRGLNKLWNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCC
ELSLEVLAKIGLEIDSDVPFCVYSRTAHVLGKGEKVIPLKKLPPMWVVIAKPKLSVSTPY
CHHHHHHHHHCCEECCCCCEEEEHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCCHH
ILSKIEHKELQHSNIESILDAIEREDYTDICRYMGNVLEQVTGKEHPEVLKIKNRIKQYG
HHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
ADAAQMSGTGPTVFGVASKSSRAKHIVNSLKGFCHEVYLVRIL
CCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MEITEKAPAKLNLFLDTPFNHPDGLPEWNMIMTSVDLADYVKIESIPGKMGIKVRTNTGF
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHEECCCHHHHEEEECCCCCCCEEEECCCCC
LPNDSRNLAYQAARILQEKYHVRAKVIIEIRKHIPVAAGLGGGSSDAAAVLRGLNKLWNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCC
ELSLEVLAKIGLEIDSDVPFCVYSRTAHVLGKGEKVIPLKKLPPMWVVIAKPKLSVSTPY
CHHHHHHHHHCCEECCCCCEEEEHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCCHH
ILSKIEHKELQHSNIESILDAIEREDYTDICRYMGNVLEQVTGKEHPEVLKIKNRIKQYG
HHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
ADAAQMSGTGPTVFGVASKSSRAKHIVNSLKGFCHEVYLVRIL
CCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA