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The map label for this gene is rsmA

Identifier: 116492073

GI number: 116492073

Start: 316041

End: 316934

Strand: Direct

Name: rsmA

Synonym: PEPE_0269

Alternate gene names: 116492073

Gene position: 316041-316934 (Clockwise)

Preceding gene: 116492072

Following gene: 116492074

Centisome position: 17.25

GC content: 38.93

Gene sequence:

>894_bases
ATGAATAATCAAATGGATATTGGTAATCCAACTCGAACTCGAGCTATCTTAGAAAAATATGGATTATCAGCTAAGAAAAG
TCTAGGACAAAACTTTCTGACTGACCCTAACGTACTTTTAAATATTGTGGATGCTGCGGAAGTATCTCCTGAGGATGACG
TGATTGAAGTTGGACCCGGAATTGGTAGTTTAACTGAACAAATTGCCAAACGTGCTCATCATGTCTTAGCATTTGAAATT
GATCGTAATCTAATGAACGTCCTAGATGAAACCCTTAGCCCTTACGATAATATCACGGTGGTTAATCAAGACGTTTTAAA
AGCAAATGTAAACGAAGAAGTCGAAAATCATTTAGACGGAAAACGTCGTTTGAAATTGGTAGCTAACTTACCATATTACA
TTACAACCCCCATTTTAAAGACATTTATGGCTAGTACCCTGCCCATCGATAAAATGGTCGTTATGATGCAAAAAGAGGTC
GCAGAACGCCTTACAGCCCAACCTGGCGACAAAGAATATGGTTCCTTATCAGTAGTGGTTCAATATCGAATGAATACGCA
AATTGAATTTGATGTTTCTTCTAAGGTATTTGTGCCGCGTCCTAAGGTCGATTCAGCCATCGTGAGTCTTACGCCTCGTG
CTGGCTGGGAAGTAATGCCAGAAGACGACAAAGACTTCTTTAAGACGGTTCACGGATGCTTTATGCACCGTCGTAAAAAT
ATTTGGAACAATATGCAGGGACTTTATGGTAAAGAACCGGAAATCAAAGCTAAAATCCAAAATGTTTTGGATGATTTAGG
TATTGATCCACAAGTGCGACCAGAGCGATTAACCGTGCTGGATTTCATTAAATTGCACAATCGGATTCAAATTGAAGGTC
TTAAACGAAAATAA

Upstream 100 bases:

>100_bases
GGATACGTAAATGCCAAACAACTACCTAAAAGGTTACAACTGTTCCAAGTTGAAAAATCAGATTTTAATCATGCTGTAGA
ACAAATTAATGGAGGAATTA

Downstream 100 bases:

>100_bases
TCCGTTTTCATGGGCTTGAAGCATAAAAAAATGAAATGTAAAGCGTTGATATTAAATTGTACGTGTGGTAAAATTGCAAA
CTTTGTGAAACTATGATAGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNNQMDIGNPTRTRAILEKYGLSAKKSLGQNFLTDPNVLLNIVDAAEVSPEDDVIEVGPGIGSLTEQIAKRAHHVLAFEI
DRNLMNVLDETLSPYDNITVVNQDVLKANVNEEVENHLDGKRRLKLVANLPYYITTPILKTFMASTLPIDKMVVMMQKEV
AERLTAQPGDKEYGSLSVVVQYRMNTQIEFDVSSKVFVPRPKVDSAIVSLTPRAGWEVMPEDDKDFFKTVHGCFMHRRKN
IWNNMQGLYGKEPEIKAKIQNVLDDLGIDPQVRPERLTVLDFIKLHNRIQIEGLKRK

Sequences:

>Translated_297_residues
MNNQMDIGNPTRTRAILEKYGLSAKKSLGQNFLTDPNVLLNIVDAAEVSPEDDVIEVGPGIGSLTEQIAKRAHHVLAFEI
DRNLMNVLDETLSPYDNITVVNQDVLKANVNEEVENHLDGKRRLKLVANLPYYITTPILKTFMASTLPIDKMVVMMQKEV
AERLTAQPGDKEYGSLSVVVQYRMNTQIEFDVSSKVFVPRPKVDSAIVSLTPRAGWEVMPEDDKDFFKTVHGCFMHRRKN
IWNNMQGLYGKEPEIKAKIQNVLDDLGIDPQVRPERLTVLDFIKLHNRIQIEGLKRK
>Mature_297_residues
MNNQMDIGNPTRTRAILEKYGLSAKKSLGQNFLTDPNVLLNIVDAAEVSPEDDVIEVGPGIGSLTEQIAKRAHHVLAFEI
DRNLMNVLDETLSPYDNITVVNQDVLKANVNEEVENHLDGKRRLKLVANLPYYITTPILKTFMASTLPIDKMVVMMQKEV
AERLTAQPGDKEYGSLSVVVQYRMNTQIEFDVSSKVFVPRPKVDSAIVSLTPRAGWEVMPEDDKDFFKTVHGCFMHRRKN
IWNNMQGLYGKEPEIKAKIQNVLDDLGIDPQVRPERLTVLDFIKLHNRIQIEGLKRK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=291, Percent_Identity=29.8969072164948, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI7657198, Length=296, Percent_Identity=28.0405405405405, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786236, Length=272, Percent_Identity=35.2941176470588, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI25146882, Length=217, Percent_Identity=35.0230414746544, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=301, Percent_Identity=27.2425249169435, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=186, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357273, Length=213, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21358017, Length=200, Percent_Identity=33, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PEDPA (Q03HF6)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803808.1
- ProteinModelPortal:   Q03HF6
- SMR:   Q03HF6
- STRING:   Q03HF6
- GeneID:   4418546
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0269
- NMPDR:   fig|278197.10.peg.245
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- PhylomeDB:   Q03HF6
- BioCyc:   PPEN278197:PEPE_0269-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 33708; Mature: 33708

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 31-31 BINDING 33-33 BINDING 58-58 BINDING 79-79 BINDING 104-104 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQMDIGNPTRTRAILEKYGLSAKKSLGQNFLTDPNVLLNIVDAAEVSPEDDVIEVGPG
CCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHEEHHHHCCCCCCCEEEECCC
IGSLTEQIAKRAHHVLAFEIDRNLMNVLDETLSPYDNITVVNQDVLKANVNEEVENHLDG
CCHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHHHHHCCH
KRRLKLVANLPYYITTPILKTFMASTLPIDKMVVMMQKEVAERLTAQPGDKEYGSLSVVV
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
QYRMNTQIEFDVSSKVFVPRPKVDSAIVSLTPRAGWEVMPEDDKDFFKTVHGCFMHRRKN
EEECCCEEEEECCCCEECCCCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IWNNMQGLYGKEPEIKAKIQNVLDDLGIDPQVRPERLTVLDFIKLHNRIQIEGLKRK
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCC
>Mature Secondary Structure
MNNQMDIGNPTRTRAILEKYGLSAKKSLGQNFLTDPNVLLNIVDAAEVSPEDDVIEVGPG
CCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHEEHHHHCCCCCCCEEEECCC
IGSLTEQIAKRAHHVLAFEIDRNLMNVLDETLSPYDNITVVNQDVLKANVNEEVENHLDG
CCHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHHHHHCCH
KRRLKLVANLPYYITTPILKTFMASTLPIDKMVVMMQKEVAERLTAQPGDKEYGSLSVVV
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
QYRMNTQIEFDVSSKVFVPRPKVDSAIVSLTPRAGWEVMPEDDKDFFKTVHGCFMHRRKN
EEECCCEEEEECCCCEECCCCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IWNNMQGLYGKEPEIKAKIQNVLDDLGIDPQVRPERLTVLDFIKLHNRIQIEGLKRK
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA