Click here to switch to the map view.

The map label for this gene is rep [H]

Identifier: 116515298

GI number: 116515298

Start: 404176

End: 406122

Strand: Reverse

Name: rep [H]

Synonym: BCc_387

Alternate gene names: 116515298

Gene position: 406122-404176 (Counterclockwise)

Preceding gene: 116515299

Following gene: 116515297

Centisome position: 97.54

GC content: 19.67

Gene sequence:

>1947_bases
ATGATATTAAATAAAATTCAAAAAAAAGCTATTCATTTGATTAATAATCCATGTTTAATTTTAGCAGGTGCGGGTTCAGG
AAAAACAAGCGTTATAATAAATAAAATAATAGTATTAATAAAAATTTACGGTTATAATCCTAAAAAAATTTTTGCAATTA
CATTTACTAATAAAGCTGCAAAAGAAATTAAATTACGATTAGTTAATCAATTAACTATGAAACAAATAAAAGAAATTTCA
ATATCTACTTTTCATTCATTAGGACTTAAAATTATTAGAAATGAATATAAATTTCTAGGATTAATTGAAAATTTCACATT
ATTTGATGAAAAAGAACAATTGCTGCTTTTAAAAAGTTTTATATCTGATTTGGATAAAAATTATAATATATTTTTTTTAA
AACAGTTGTTATATCAAATTTCTTTTTGGAAAAATAATTTATTAGATGCTAAATTAGCACGTTTAAAAATTTCATCAATG
TTAGAGGAAAAATGTATTTTCTTTTATGAAAAGTATGAATTTTTTTTAAAAGAACATAATATATTAGATTTTCATGATTT
AATTTTTTTGCCAACAAATTTATTAAAAAAAAATATAAATATTCAATTAAAATGGCAAAAAAAAATTCAATATTTATTAA
TTGATGAATATCAAGATGTTAATAGTAGTCAGTATAAATTAATTAAATTATTATGTGGATATAATTCTAATTTAACCGTA
GTGGGTGATAATGATCAATCTATTTATTCGTGGAGAGGTGCTGAAACAAAGATATTTTATTTATTAAAAAATGATTTTCC
AAATTTGAATATTTTAAAAATGGAACAAAATTATCGTTCTTCTGGAAGAATATTACATGTAGCTAATATATTAATTTCTC
ATAATATAAATTTTTTTCATAAAAAATTGTTTTCAAAATTAGATTATGGTAATAAAATTTATATTTTAAAATCAATGAAT
GAAATACATGAAGCTCAACAAATTATACAATATATAAAATTTCATAAAAATAAATATCAAGGAAGATATAATGATTATGC
TATTTTATATCGTAGTAATTATCAAGTTAGAGCAGTAGAAACAGAATTAATTTATCAAAATATTCCGTATTTAATTCATT
CTGGATGTTCTTTTTTTGATTTGTTAGAAATTAAAGATTTATTAGCATATTTACGTTTAGTAATTAATCAAAATGATGAT
TTTGCATTTCTACGAATTATTAATGTTCCTAATAGAAAAATTGGTTTAATTACTATTTCAAAATTAAAAAAATTTGCAAA
AAATAATAAAATTAGTTTATTTGTAGCTAGTACAGATAATCGTATGAAATTACAATTAAGAAAAAATATTATTAATACAT
TAAATAATTTTGTTGTATGGATTTTAAAATTAACTTTAATTTTGTCTAAAAAACCTGAAAAAATATTAGAATATATTATT
CGTGATACAAAATATTTTTCTTGGATTCAAAATTTTTATACTGATTCAGTTATTATAAAAAAAAAAAAAAAAAATATAGT
TTTTTTTTCAAAATGGTTAAAAAAAATGTTAATCGGAAATGATTTCCATATTCCTATGAAATTAGAAGATATATTAATTC
ATTTTATTTGTGGTGATCTCGATTATTCATCATTTAATACAAAAAAAAAAAGATTAGATAAATTGCAATTAATGACTATA
CATTCTTCAAAAGGATTAGAATTTCCTGTGGTATGTATTATTGGAATGGAGGAAGGTACATTACCTCATCAAAAAAGTAT
TCTTGATAATAATATTACTGAAGAACGTCGTTTATTATATGTAGGTATTACAAGAGCAAAAAGTCAATTATTTCTTAGTT
TTTGTAAAAAAAAAAGAAAATTTGGAATGATTATGCATTTACAACCGAGTCGATTTTTATTCGAATTACCAAAACGTGAA
ATTATTTGGATTAAAAATATTATTTAA

Upstream 100 bases:

>100_bases
ATATTATTTTTAATAAAAATTAATTTTATTATAAATTTTTTTTATTTTTTATGTTTTTTATATTTTAATTTTAAAAAATT
ATTATTCTGATAATATATTT

Downstream 100 bases:

>100_bases
AATAAATATTTTAAATAATATTTTAAGAAATTAGTGCATTTAGTAATTTAATTATTTTATATTATTAAAATAATGAAATA
ATATTTAAATGTATTAAATT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MILNKIQKKAIHLINNPCLILAGAGSGKTSVIINKIIVLIKIYGYNPKKIFAITFTNKAAKEIKLRLVNQLTMKQIKEIS
ISTFHSLGLKIIRNEYKFLGLIENFTLFDEKEQLLLLKSFISDLDKNYNIFFLKQLLYQISFWKNNLLDAKLARLKISSM
LEEKCIFFYEKYEFFLKEHNILDFHDLIFLPTNLLKKNINIQLKWQKKIQYLLIDEYQDVNSSQYKLIKLLCGYNSNLTV
VGDNDQSIYSWRGAETKIFYLLKNDFPNLNILKMEQNYRSSGRILHVANILISHNINFFHKKLFSKLDYGNKIYILKSMN
EIHEAQQIIQYIKFHKNKYQGRYNDYAILYRSNYQVRAVETELIYQNIPYLIHSGCSFFDLLEIKDLLAYLRLVINQNDD
FAFLRIINVPNRKIGLITISKLKKFAKNNKISLFVASTDNRMKLQLRKNIINTLNNFVVWILKLTLILSKKPEKILEYII
RDTKYFSWIQNFYTDSVIIKKKKKNIVFFSKWLKKMLIGNDFHIPMKLEDILIHFICGDLDYSSFNTKKKRLDKLQLMTI
HSSKGLEFPVVCIIGMEEGTLPHQKSILDNNITEERRLLYVGITRAKSQLFLSFCKKKRKFGMIMHLQPSRFLFELPKRE
IIWIKNII

Sequences:

>Translated_648_residues
MILNKIQKKAIHLINNPCLILAGAGSGKTSVIINKIIVLIKIYGYNPKKIFAITFTNKAAKEIKLRLVNQLTMKQIKEIS
ISTFHSLGLKIIRNEYKFLGLIENFTLFDEKEQLLLLKSFISDLDKNYNIFFLKQLLYQISFWKNNLLDAKLARLKISSM
LEEKCIFFYEKYEFFLKEHNILDFHDLIFLPTNLLKKNINIQLKWQKKIQYLLIDEYQDVNSSQYKLIKLLCGYNSNLTV
VGDNDQSIYSWRGAETKIFYLLKNDFPNLNILKMEQNYRSSGRILHVANILISHNINFFHKKLFSKLDYGNKIYILKSMN
EIHEAQQIIQYIKFHKNKYQGRYNDYAILYRSNYQVRAVETELIYQNIPYLIHSGCSFFDLLEIKDLLAYLRLVINQNDD
FAFLRIINVPNRKIGLITISKLKKFAKNNKISLFVASTDNRMKLQLRKNIINTLNNFVVWILKLTLILSKKPEKILEYII
RDTKYFSWIQNFYTDSVIIKKKKKNIVFFSKWLKKMLIGNDFHIPMKLEDILIHFICGDLDYSSFNTKKKRLDKLQLMTI
HSSKGLEFPVVCIIGMEEGTLPHQKSILDNNITEERRLLYVGITRAKSQLFLSFCKKKRKFGMIMHLQPSRFLFELPKRE
IIWIKNII
>Mature_648_residues
MILNKIQKKAIHLINNPCLILAGAGSGKTSVIINKIIVLIKIYGYNPKKIFAITFTNKAAKEIKLRLVNQLTMKQIKEIS
ISTFHSLGLKIIRNEYKFLGLIENFTLFDEKEQLLLLKSFISDLDKNYNIFFLKQLLYQISFWKNNLLDAKLARLKISSM
LEEKCIFFYEKYEFFLKEHNILDFHDLIFLPTNLLKKNINIQLKWQKKIQYLLIDEYQDVNSSQYKLIKLLCGYNSNLTV
VGDNDQSIYSWRGAETKIFYLLKNDFPNLNILKMEQNYRSSGRILHVANILISHNINFFHKKLFSKLDYGNKIYILKSMN
EIHEAQQIIQYIKFHKNKYQGRYNDYAILYRSNYQVRAVETELIYQNIPYLIHSGCSFFDLLEIKDLLAYLRLVINQNDD
FAFLRIINVPNRKIGLITISKLKKFAKNNKISLFVASTDNRMKLQLRKNIINTLNNFVVWILKLTLILSKKPEKILEYII
RDTKYFSWIQNFYTDSVIIKKKKKNIVFFSKWLKKMLIGNDFHIPMKLEDILIHFICGDLDYSSFNTKKKRLDKLQLMTI
HSSKGLEFPVVCIIGMEEGTLPHQKSILDNNITEERRLLYVGITRAKSQLFLSFCKKKRKFGMIMHLQPSRFLFELPKRE
IIWIKNII

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=46.3565891472868, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI2367296, Length=645, Percent_Identity=33.4883720930233, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6322369, Length=443, Percent_Identity=31.3769751693002, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324477, Length=657, Percent_Identity=23.896499238965, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76738; Mature: 76738

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNKIQKKAIHLINNPCLILAGAGSGKTSVIINKIIVLIKIYGYNPKKIFAITFTNKAA
CCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHEEEEEEECCCCCEEEEEEECCCHH
KEIKLRLVNQLTMKQIKEISISTFHSLGLKIIRNEYKFLGLIENFTLFDEKEQLLLLKSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCEEECCHHHHHHHHHH
ISDLDKNYNIFFLKQLLYQISFWKNNLLDAKLARLKISSMLEEKCIFFYEKYEFFLKEHN
HHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ILDFHDLIFLPTNLLKKNINIQLKWQKKIQYLLIDEYQDVNSSQYKLIKLLCGYNSNLTV
CCCHHHHHHHCHHHHHCCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHCCCCCCEEE
VGDNDQSIYSWRGAETKIFYLLKNDFPNLNILKMEQNYRSSGRILHVANILISHNINFFH
EECCCCCEEEECCCCEEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEHHHCCCHHHH
KKLFSKLDYGNKIYILKSMNEIHEAQQIIQYIKFHKNKYQGRYNDYAILYRSNYQVRAVE
HHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEE
TELIYQNIPYLIHSGCSFFDLLEIKDLLAYLRLVINQNDDFAFLRIINVPNRKIGLITIS
HHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEHH
KLKKFAKNNKISLFVASTDNRMKLQLRKNIINTLNNFVVWILKLTLILSKKPEKILEYII
HHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RDTKYFSWIQNFYTDSVIIKKKKKNIVFFSKWLKKMLIGNDFHIPMKLEDILIHFICGDL
HHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCC
DYSSFNTKKKRLDKLQLMTIHSSKGLEFPVVCIIGMEEGTLPHQKSILDNNITEERRLLY
CCCCCCCHHHHHCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCEEEE
VGITRAKSQLFLSFCKKKRKFGMIMHLQPSRFLFELPKREIIWIKNII
EEEHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MILNKIQKKAIHLINNPCLILAGAGSGKTSVIINKIIVLIKIYGYNPKKIFAITFTNKAA
CCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHEEEEEEECCCCCEEEEEEECCCHH
KEIKLRLVNQLTMKQIKEISISTFHSLGLKIIRNEYKFLGLIENFTLFDEKEQLLLLKSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCEEECCHHHHHHHHHH
ISDLDKNYNIFFLKQLLYQISFWKNNLLDAKLARLKISSMLEEKCIFFYEKYEFFLKEHN
HHHHCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ILDFHDLIFLPTNLLKKNINIQLKWQKKIQYLLIDEYQDVNSSQYKLIKLLCGYNSNLTV
CCCHHHHHHHCHHHHHCCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHCCCCCCEEE
VGDNDQSIYSWRGAETKIFYLLKNDFPNLNILKMEQNYRSSGRILHVANILISHNINFFH
EECCCCCEEEECCCCEEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEHHHCCCHHHH
KKLFSKLDYGNKIYILKSMNEIHEAQQIIQYIKFHKNKYQGRYNDYAILYRSNYQVRAVE
HHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEE
TELIYQNIPYLIHSGCSFFDLLEIKDLLAYLRLVINQNDDFAFLRIINVPNRKIGLITIS
HHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEHH
KLKKFAKNNKISLFVASTDNRMKLQLRKNIINTLNNFVVWILKLTLILSKKPEKILEYII
HHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RDTKYFSWIQNFYTDSVIIKKKKKNIVFFSKWLKKMLIGNDFHIPMKLEDILIHFICGDL
HHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCC
DYSSFNTKKKRLDKLQLMTIHSSKGLEFPVVCIIGMEEGTLPHQKSILDNNITEERRLLY
CCCCCCCHHHHHCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCEEEE
VGITRAKSQLFLSFCKKKRKFGMIMHLQPSRFLFELPKREIIWIKNII
EEEHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]