Click here to switch to the map view.

The map label for this gene is hslU

Identifier: 116515291

GI number: 116515291

Start: 396090

End: 397424

Strand: Direct

Name: hslU

Synonym: BCc_377

Alternate gene names: 116515291

Gene position: 396090-397424 (Clockwise)

Preceding gene: 116515290

Following gene: 116515292

Centisome position: 95.13

GC content: 26.37

Gene sequence:

>1335_bases
ATGTCAGAAATGACTCCAAGAAAAATTGTAAAAGAACTAAACAAATACATTATTGGACAAAATAATGCTAAACGTGCAGT
AGCTATTGCTTTAAGAAATCGATGGAGAAGAATGCAATTAAATTCAGAATTACGTAATGAAATTACTCCTAAAAATATTT
TAATGATCGGACCTACAGGAGTAGGTAAAACAGAAATTGCACGTAGACTAGCAAAACTAGCTAACGCACCTTTTATTAAA
GTAGAAGCAACAAAATTTACTGAAGTAGGATATGTAGGAAAAGAAGTTGATTCTATAATTCGTGATTTAACAGATTTAGC
AATAAAAATGATTCGTTTACAAATAATTAAAAAAAACAAAAAACATGCTAAAAAAAGAGCAGAAGAAAGAATATTGAAAA
TATTAATTCCTGTACCGAAAGATAATTGGAATGAAGAAAATTTAAAAGAAAAGCCAGAAAAAACAATTCAAATTTTTAGA
AAAAAACTACAAGAAGGAAAATTAGATAATAAAGAAATAGAAATACAAATTGCTGCAACACCAATTGGAATTGAAATTAT
GTCTCCTCCGGGAATGGAAGAATTAACTAATCAATTACAATCTTTATTTCAAAATTTAAGTGGAAAGAAAAAAAATTTAC
GAAAATTAAAAATAAAAGATGCAATGAAAATTATTATTGAAGAAGAAGCAGCTAAATTAATTAATTTAGAAGAATTAAAA
GAAAAAGCAATTTATTCAGTTGAACAAAATAGTATAGTTTTTATTGATGAAATTGATAAAATTTGTAAACATCATTCTTC
AGCATCAAATTCTGATGTATCACGAGAAGGTGTGCAAAGAGATTTACTACCATTAATTGAAGGATGTACTGTTTCAACTA
AACATGGATCAGTTAAAACTGATCATATATTATTTATTGCATCAGGAGCTTTTCAAACGTCTACTCCTTCTGATTTAATT
CCAGAATTACAAGGAAGACTTCCTATACGTGTAGAACTAAATGCTCTTACTGTAGATGATTTTGAAAGAATTCTAACTGA
ACCAAACGCGTCTATTACTACACAATATAAAGCATTAATAAAAACGGAAGGAGTAGATATAATTTTTACAAAAAAAGGAA
TACGAAAAATTGCAGAAGCTTCTTGGAAAATAAATGAATCTATGGAAAATATTGGAGCACGAAGATTATATACTGTATTA
GAAAAATTAATGGAAGATATTTCTTTTAATTCTAATGAAAAATTTGGACAAAAAATTTATATTGACGAAAAATATGTTAA
TCTACATTTAGATAAACTAATAGAAAATGAAGATTTAAGTCGATTTATTTTATAA

Upstream 100 bases:

>100_bases
GTAAAAAAATCACTGCAAATCGCTTCAAACATTTGTATCTACACTAACCAATCATTTACTATAAAAGAAATAAAATCAGA
AAAGTAATAAGGATACTTAC

Downstream 100 bases:

>100_bases
TAAATATACTTTCAAATATATATATATATATATTTAAATGATTACCTATCTTAGACTAATTTATCTATGATAGGTAATCA
TTTAATTAGAATTAAATATT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSEMTPRKIVKELNKYIIGQNNAKRAVAIALRNRWRRMQLNSELRNEITPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVDSIIRDLTDLAIKMIRLQIIKKNKKHAKKRAEERILKILIPVPKDNWNEENLKEKPEKTIQIFR
KKLQEGKLDNKEIEIQIAATPIGIEIMSPPGMEELTNQLQSLFQNLSGKKKNLRKLKIKDAMKIIIEEEAAKLINLEELK
EKAIYSVEQNSIVFIDEIDKICKHHSSASNSDVSREGVQRDLLPLIEGCTVSTKHGSVKTDHILFIASGAFQTSTPSDLI
PELQGRLPIRVELNALTVDDFERILTEPNASITTQYKALIKTEGVDIIFTKKGIRKIAEASWKINESMENIGARRLYTVL
EKLMEDISFNSNEKFGQKIYIDEKYVNLHLDKLIENEDLSRFIL

Sequences:

>Translated_444_residues
MSEMTPRKIVKELNKYIIGQNNAKRAVAIALRNRWRRMQLNSELRNEITPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVDSIIRDLTDLAIKMIRLQIIKKNKKHAKKRAEERILKILIPVPKDNWNEENLKEKPEKTIQIFR
KKLQEGKLDNKEIEIQIAATPIGIEIMSPPGMEELTNQLQSLFQNLSGKKKNLRKLKIKDAMKIIIEEEAAKLINLEELK
EKAIYSVEQNSIVFIDEIDKICKHHSSASNSDVSREGVQRDLLPLIEGCTVSTKHGSVKTDHILFIASGAFQTSTPSDLI
PELQGRLPIRVELNALTVDDFERILTEPNASITTQYKALIKTEGVDIIFTKKGIRKIAEASWKINESMENIGARRLYTVL
EKLMEDISFNSNEKFGQKIYIDEKYVNLHLDKLIENEDLSRFIL
>Mature_443_residues
SEMTPRKIVKELNKYIIGQNNAKRAVAIALRNRWRRMQLNSELRNEITPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVDSIIRDLTDLAIKMIRLQIIKKNKKHAKKRAEERILKILIPVPKDNWNEENLKEKPEKTIQIFRK
KLQEGKLDNKEIEIQIAATPIGIEIMSPPGMEELTNQLQSLFQNLSGKKKNLRKLKIKDAMKIIIEEEAAKLINLEELKE
KAIYSVEQNSIVFIDEIDKICKHHSSASNSDVSREGVQRDLLPLIEGCTVSTKHGSVKTDHILFIASGAFQTSTPSDLIP
ELQGRLPIRVELNALTVDDFERILTEPNASITTQYKALIKTEGVDIIFTKKGIRKIAEASWKINESMENIGARRLYTVLE
KLMEDISFNSNEKFGQKIYIDEKYVNLHLDKLIENEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=444, Percent_Identity=74.5495495495496, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=101, Percent_Identity=45.5445544554455, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=123, Percent_Identity=34.1463414634146, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_BUCCC (Q056X2)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802920.1
- ProteinModelPortal:   Q056X2
- SMR:   Q056X2
- STRING:   Q056X2
- EnsemblBacteria:   EBBUCT00000001531
- GeneID:   4440968
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_377
- eggNOG:   COG1220
- GeneTree:   EBGT00050000007932
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   BAPH372461:BCC_377-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2

EC number: NA

Molecular weight: Translated: 50730; Mature: 50599

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 256-256 BINDING 322-322 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPRKIVKELNKYIIGQNNAKRAVAIALRNRWRRMQLNSELRNEITPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDLAIKMIRLQIIKKNK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHAKKRAEERILKILIPVPKDNWNEENLKEKPEKTIQIFRKKLQEGKLDNKEIEIQIAAT
HHHHHHHHHHHHEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
PIGIEIMSPPGMEELTNQLQSLFQNLSGKKKNLRKLKIKDAMKIIIEEEAAKLINLEELK
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EKAIYSVEQNSIVFIDEIDKICKHHSSASNSDVSREGVQRDLLPLIEGCTVSTKHGSVKT
HHHHHEECCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCC
DHILFIASGAFQTSTPSDLIPELQGRLPIRVELNALTVDDFERILTEPNASITTQYKALI
CEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEEHHHHHHHHCCCCCCHHHHHHHHH
KTEGVDIIFTKKGIRKIAEASWKINESMENIGARRLYTVLEKLMEDISFNSNEKFGQKIY
HCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IDEKYVNLHLDKLIENEDLSRFIL
EEHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SEMTPRKIVKELNKYIIGQNNAKRAVAIALRNRWRRMQLNSELRNEITPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDLAIKMIRLQIIKKNK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHAKKRAEERILKILIPVPKDNWNEENLKEKPEKTIQIFRKKLQEGKLDNKEIEIQIAAT
HHHHHHHHHHHHEEEEECCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
PIGIEIMSPPGMEELTNQLQSLFQNLSGKKKNLRKLKIKDAMKIIIEEEAAKLINLEELK
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EKAIYSVEQNSIVFIDEIDKICKHHSSASNSDVSREGVQRDLLPLIEGCTVSTKHGSVKT
HHHHHEECCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCC
DHILFIASGAFQTSTPSDLIPELQGRLPIRVELNALTVDDFERILTEPNASITTQYKALI
CEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEEHHHHHHHHCCCCCCHHHHHHHHH
KTEGVDIIFTKKGIRKIAEASWKINESMENIGARRLYTVLEKLMEDISFNSNEKFGQKIY
HCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IDEKYVNLHLDKLIENEDLSRFIL
EEHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA