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The map label for this gene is bioH [H]

Identifier: 116515273

GI number: 116515273

Start: 379835

End: 380623

Strand: Direct

Name: bioH [H]

Synonym: BCc_356

Alternate gene names: 116515273

Gene position: 379835-380623 (Clockwise)

Preceding gene: 116515232

Following gene: 116515275

Centisome position: 91.22

GC content: 15.46

Gene sequence:

>789_bases
ATGAATCAAAAAAAAAAAATTAATTTATTTACTATTGAAAAAGGAAAAATTCACTTAGTATTTTTTCATGGTTGGGGATT
AAATTCTCTTATATGGAAAAAAATTATTCCAATTTTAAAAAAAAAATTTACTCTGTATTTTCTTGATTTTCCAGGATATG
GTAAAAATATTGATTTTCCAATAATGAATTTTAATCAATTATCTGATTATTTATTTAAAAAAATAAAAAAAAAAGTTATT
TTAATAGGTTGGTCTTTAGGTGGACAATTTGCACATTATTTAAGTTTCAAATATCCAAATTTTATTTTAGCTGTTATTTA
TATAACTTATACACCATTTTTTATGAAAAAAAAAAGAAAAAAGAAAAAAATATTTAAAAAAATAAAAAATGATATAATTT
TAAATTATAAAAAATTTTTATATGATTTTATTAATCTACATATTTTATATAAAAAAAAAAAAAATCTTTTATATTTTAAA
AAAAAATTTTCTTTTTTAAAAAAATATCCTAATCCAAAAAAAGAAGCAATTGAAATAGGATATAAATGGTTAACTAAAAT
TGATCAAAGAAAAAAAATACTATCTAAAAATATCCCTACATTAAAAATATATGGTGAATTAGATAATCTTATATCAATTA
GAATATATAAAAAATCAAATAATTTAAATAATTTAAATAAAATTAAAAAAAATTATTATTATATAATTCCTAAAGCTAGA
CATGCACCATTTTTATCTCATCCTAAGATTTTTTGCAAAATAATTAATAAATTTATCAAAAAATTATAA

Upstream 100 bases:

>100_bases
TTTTTATCATAGAAACTCTCTATATAATATGTTATTATTATTTTTAAAAAAATAAATTTATATAAAAAATTATATTTATA
TCATTTTACAAATTTTATAT

Downstream 100 bases:

>100_bases
AATAATATACCAAAATATAGTAATTAAAAAAAATTATTTACTGCAATATCAAAAAGGAATATCTTCATCATCAAAATCAA
TTTTAGATGTAGAATCTACT

Product: BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNQKKKINLFTIEKGKIHLVFFHGWGLNSLIWKKIIPILKKKFTLYFLDFPGYGKNIDFPIMNFNQLSDYLFKKIKKKVI
LIGWSLGGQFAHYLSFKYPNFILAVIYITYTPFFMKKKRKKKKIFKKIKNDIILNYKKFLYDFINLHILYKKKKNLLYFK
KKFSFLKKYPNPKKEAIEIGYKWLTKIDQRKKILSKNIPTLKIYGELDNLISIRIYKKSNNLNNLNKIKKNYYYIIPKAR
HAPFLSHPKIFCKIINKFIKKL

Sequences:

>Translated_262_residues
MNQKKKINLFTIEKGKIHLVFFHGWGLNSLIWKKIIPILKKKFTLYFLDFPGYGKNIDFPIMNFNQLSDYLFKKIKKKVI
LIGWSLGGQFAHYLSFKYPNFILAVIYITYTPFFMKKKRKKKKIFKKIKNDIILNYKKFLYDFINLHILYKKKKNLLYFK
KKFSFLKKYPNPKKEAIEIGYKWLTKIDQRKKILSKNIPTLKIYGELDNLISIRIYKKSNNLNNLNKIKKNYYYIIPKAR
HAPFLSHPKIFCKIINKFIKKL
>Mature_262_residues
MNQKKKINLFTIEKGKIHLVFFHGWGLNSLIWKKIIPILKKKFTLYFLDFPGYGKNIDFPIMNFNQLSDYLFKKIKKKVI
LIGWSLGGQFAHYLSFKYPNFILAVIYITYTPFFMKKKRKKKKIFKKIKNDIILNYKKFLYDFINLHILYKKKKNLLYFK
KKFSFLKKYPNPKKEAIEIGYKWLTKIDQRKKILSKNIPTLKIYGELDNLISIRIYKKSNNLNNLNKIKKNYYYIIPKAR
HAPFLSHPKIFCKIINKFIKKL

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=252, Percent_Identity=28.1746031746032, Blast_Score=141, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 31717; Mature: 31717

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKKKINLFTIEKGKIHLVFFHGWGLNSLIWKKIIPILKKKFTLYFLDFPGYGKNIDFP
CCCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCC
IMNFNQLSDYLFKKIKKKVILIGWSLGGQFAHYLSFKYPNFILAVIYITYTPFFMKKKRK
CCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
KKKIFKKIKNDIILNYKKFLYDFINLHILYKKKKNLLYFKKKFSFLKKYPNPKKEAIEIG
HHHHHHHHHHHHEEHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHH
YKWLTKIDQRKKILSKNIPTLKIYGELDNLISIRIYKKSNNLNNLNKIKKNYYYIIPKAR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHCCCEEEEEECCC
HAPFLSHPKIFCKIINKFIKKL
CCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQKKKINLFTIEKGKIHLVFFHGWGLNSLIWKKIIPILKKKFTLYFLDFPGYGKNIDFP
CCCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCC
IMNFNQLSDYLFKKIKKKVILIGWSLGGQFAHYLSFKYPNFILAVIYITYTPFFMKKKRK
CCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHH
KKKIFKKIKNDIILNYKKFLYDFINLHILYKKKKNLLYFKKKFSFLKKYPNPKKEAIEIG
HHHHHHHHHHHHEEHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHH
YKWLTKIDQRKKILSKNIPTLKIYGELDNLISIRIYKKSNNLNNLNKIKKNYYYIIPKAR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHCCCEEEEEECCC
HAPFLSHPKIFCKIINKFIKKL
CCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA