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The map label for this gene is dusA [H]

Identifier: 116515275

GI number: 116515275

Start: 381419

End: 382465

Strand: Direct

Name: dusA [H]

Synonym: BCc_358

Alternate gene names: 116515275

Gene position: 381419-382465 (Clockwise)

Preceding gene: 116515273

Following gene: 116515276

Centisome position: 91.6

GC content: 17.19

Gene sequence:

>1047_bases
ATGATATTCTTATATATTATAAATATATTATATCATATTCACTATATTAAAATTAATAATATTAAAATGAAAAAAAAATA
TTCAAATAAATTCTCAGTTGCTCCAATGTTTAAATATACTGATCAACATTGCTTATATTTTTATAGACAACTAACAAAAA
AAACATTTTTGTATACAGAAATGATAACTATACAAAAAATGTTATTTTCTAAAAAATTATTTAAAAAAAAACAAATCAAA
AAAAATAATCCTATAGCAATACAATTAGCAGGAAATAACCCACTATTTCTTTCATTATGTGCAAAAAAAGCATATTTTCT
AGGATTTAAAGAAATAAATTTAAATATTGGTTGTCCATCTAAAAAAGCTAAATCTGGAAATTTTGGTATATATTTAATGA
ATAATCCTATTTTAGTATATAATTTAATTAAAGCTATATATTTAACTGTACCTATACAAATTAGTATAAAAATTAGATTA
GGTACTAATGAAAATAAAAATTATAGATTTCTTAAAAATTTTATTCAATTAGTTTCAAAAAATAATTACTGTATAAAATA
TATTATACATGCAAGAATTGCTAATTTGCAAATAAATAGTCCAAAAAAAAATCGAAATATTCCGTTACTAAATTATTCAT
ATGTGTATAAAATAAAAAAAGATTTTCCACATTTAATAATTATTATTAATGGAGGGATAAAAACAATTAATGAAATTAAT
GAACATTTAAAAAATGTTGATGGAGTAATGATGGGAAGGGAAATATATAAAAATCCATTATTTTTAAGAAAAATTGATAA
AAAGATTTTTTTTCAAAAAAAACAAATAAAAATAAAAAAATTCATAAAAAAAATGTCAAATTATATTAAAAAAAAAATTA
TTAAAGGTGTTCCTGCAATAAAAATTATAAAACATATGCTAAACATTTTTTATAAACTACCACAAGCAAAAAAATGGAAA
CATGAATTAATACAAAAAACAATTTATAAAAATCAAATAGATGATATATTTAATAAAATACATAAATTATTTGATAAAAA
ATTTTAA

Upstream 100 bases:

>100_bases
TTGATATTAATATTAAAATATTAAATACACTTTTTTTTTATATATTAATTTTTATATTTTAATAAAATTAAAAATTTAAA
TTTATTTAAAAATAAAAAAT

Downstream 100 bases:

>100_bases
TCTTAATTATTTAAAATTTAAAAAATAAAAAATCACTTAAATATTATAAGAAAATATTTAAAAAAATTAAATCAAAAAAA
AAAAATTTTAAAAATATTTG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MIFLYIINILYHIHYIKINNIKMKKKYSNKFSVAPMFKYTDQHCLYFYRQLTKKTFLYTEMITIQKMLFSKKLFKKKQIK
KNNPIAIQLAGNNPLFLSLCAKKAYFLGFKEINLNIGCPSKKAKSGNFGIYLMNNPILVYNLIKAIYLTVPIQISIKIRL
GTNENKNYRFLKNFIQLVSKNNYCIKYIIHARIANLQINSPKKNRNIPLLNYSYVYKIKKDFPHLIIIINGGIKTINEIN
EHLKNVDGVMMGREIYKNPLFLRKIDKKIFFQKKQIKIKKFIKKMSNYIKKKIIKGVPAIKIIKHMLNIFYKLPQAKKWK
HELIQKTIYKNQIDDIFNKIHKLFDKKF

Sequences:

>Translated_348_residues
MIFLYIINILYHIHYIKINNIKMKKKYSNKFSVAPMFKYTDQHCLYFYRQLTKKTFLYTEMITIQKMLFSKKLFKKKQIK
KNNPIAIQLAGNNPLFLSLCAKKAYFLGFKEINLNIGCPSKKAKSGNFGIYLMNNPILVYNLIKAIYLTVPIQISIKIRL
GTNENKNYRFLKNFIQLVSKNNYCIKYIIHARIANLQINSPKKNRNIPLLNYSYVYKIKKDFPHLIIIINGGIKTINEIN
EHLKNVDGVMMGREIYKNPLFLRKIDKKIFFQKKQIKIKKFIKKMSNYIKKKIIKGVPAIKIIKHMLNIFYKLPQAKKWK
HELIQKTIYKNQIDDIFNKIHKLFDKKF
>Mature_348_residues
MIFLYIINILYHIHYIKINNIKMKKKYSNKFSVAPMFKYTDQHCLYFYRQLTKKTFLYTEMITIQKMLFSKKLFKKKQIK
KNNPIAIQLAGNNPLFLSLCAKKAYFLGFKEINLNIGCPSKKAKSGNFGIYLMNNPILVYNLIKAIYLTVPIQISIKIRL
GTNENKNYRFLKNFIQLVSKNNYCIKYIIHARIANLQINSPKKNRNIPLLNYSYVYKIKKDFPHLIIIINGGIKTINEIN
EHLKNVDGVMMGREIYKNPLFLRKIDKKIFFQKKQIKIKKFIKKMSNYIKKKIIKGVPAIKIIKHMLNIFYKLPQAKKWK
HELIQKTIYKNQIDDIFNKIHKLFDKKF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693211, Length=305, Percent_Identity=41.9672131147541, Blast_Score=280, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6323560, Length=240, Percent_Identity=25, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 41495; Mature: 41495

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLYIINILYHIHYIKINNIKMKKKYSNKFSVAPMFKYTDQHCLYFYRQLTKKTFLYTE
CCHHHHHHHHHHEEEEEEECEEEEHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
MITIQKMLFSKKLFKKKQIKKNNPIAIQLAGNNPLFLSLCAKKAYFLGFKEINLNIGCPS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHEEEEEEEEEECCCC
KKAKSGNFGIYLMNNPILVYNLIKAIYLTVPIQISIKIRLGTNENKNYRFLKNFIQLVSK
CCCCCCCEEEEEECCCCHHHHHHHHHHEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCC
NNYCIKYIIHARIANLQINSPKKNRNIPLLNYSYVYKIKKDFPHLIIIINGGIKTINEIN
CCCEEEEEEEEHEEEEEECCCCCCCCCCEEEHHEEEEEECCCCEEEEEEECCCHHHHHHH
EHLKNVDGVMMGREIYKNPLFLRKIDKKIFFQKKQIKIKKFIKKMSNYIKKKIIKGVPAI
HHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KIIKHMLNIFYKLPQAKKWKHELIQKTIYKNQIDDIFNKIHKLFDKKF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIFLYIINILYHIHYIKINNIKMKKKYSNKFSVAPMFKYTDQHCLYFYRQLTKKTFLYTE
CCHHHHHHHHHHEEEEEEECEEEEHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
MITIQKMLFSKKLFKKKQIKKNNPIAIQLAGNNPLFLSLCAKKAYFLGFKEINLNIGCPS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHEEEEEEEEEECCCC
KKAKSGNFGIYLMNNPILVYNLIKAIYLTVPIQISIKIRLGTNENKNYRFLKNFIQLVSK
CCCCCCCEEEEEECCCCHHHHHHHHHHEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCC
NNYCIKYIIHARIANLQINSPKKNRNIPLLNYSYVYKIKKDFPHLIIIINGGIKTINEIN
CCCEEEEEEEEHEEEEEECCCCCCCCCCEEEHHEEEEEECCCCEEEEEEECCCHHHHHHH
EHLKNVDGVMMGREIYKNPLFLRKIDKKIFFQKKQIKIKKFIKKMSNYIKKKIIKGVPAI
HHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KIIKHMLNIFYKLPQAKKWKHELIQKTIYKNQIDDIFNKIHKLFDKKF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]