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The map label for this gene is yheN [H]

Identifier: 116515267

GI number: 116515267

Start: 374573

End: 374965

Strand: Reverse

Name: yheN [H]

Synonym: BCc_349

Alternate gene names: 116515267

Gene position: 374965-374573 (Counterclockwise)

Preceding gene: 116515268

Following gene: 116515266

Centisome position: 90.05

GC content: 22.9

Gene sequence:

>393_bases
ATGAATTATTTAATTTTTGTTTGTGCGCCACCATATAGCATGCAAAATTCTATTACTGCTTATATGTTTGCTAAATCTTT
ATTTTTATTAAATTATCGCGTAAAAAAAGTTTTTTTTTACGCTTCTGGTATTTATAATGCTAATTGTATGATTCCTACTG
AAGAAAATGAATTTAATATTTTAAATGAATGGATCAATTTAAAAAAAAAATATAATTTTAAGCTATGTGTATGTCCTAGT
GCTGCTTATAAAAGAGGATTATTATCTAATACACATACATCAACTTTAAAGTTAGGATATAAATATAATTTTTTTTCTTC
TTCATTTCAATGGATGAGTTTAAGTGAATTATCTTATTCAATTCATGATTATGATCGTATTATACAGTTTTAA

Upstream 100 bases:

>100_bases
TATTATTTTATATAAATTTATTACTTAAAATAAGTATTTTATTTAGTTTTAAATTTTAAATAATTTTTTAAAAAAATTTT
TTTTAAAAAAATGGCTATTT

Downstream 100 bases:

>100_bases
ATTTTTTATTATATTAAATTTTATGAAATCTATCGCATTTATTTTTTCAAATTCACCATATGGAAATTCTATTAGTAAAG
AAGGTCTTGATGTTGTTCTT

Product: YheN

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D [H]

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MNYLIFVCAPPYSMQNSITAYMFAKSLFLLNYRVKKVFFYASGIYNANCMIPTEENEFNILNEWINLKKKYNFKLCVCPS
AAYKRGLLSNTHTSTLKLGYKYNFFSSSFQWMSLSELSYSIHDYDRIIQF

Sequences:

>Translated_130_residues
MNYLIFVCAPPYSMQNSITAYMFAKSLFLLNYRVKKVFFYASGIYNANCMIPTEENEFNILNEWINLKKKYNFKLCVCPS
AAYKRGLLSNTHTSTLKLGYKYNFFSSSFQWMSLSELSYSIHDYDRIIQF
>Mature_130_residues
MNYLIFVCAPPYSMQNSITAYMFAKSLFLLNYRVKKVFFYASGIYNANCMIPTEENEFNILNEWINLKKKYNFKLCVCPS
AAYKRGLLSNTHTSTLKLGYKYNFFSSSFQWMSLSELSYSIHDYDRIIQF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE [H]

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family [H]

Homologues:

Organism=Escherichia coli, GI1789743, Length=130, Percent_Identity=30, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787
- InterPro:   IPR017463 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: NA

Molecular weight: Translated: 15358; Mature: 15358

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLIFVCAPPYSMQNSITAYMFAKSLFLLNYRVKKVFFYASGIYNANCMIPTEENEFNI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCEECCEEEEECCCCCHHH
LNEWINLKKKYNFKLCVCPSAAYKRGLLSNTHTSTLKLGYKYNFFSSSFQWMSLSELSYS
HHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEHHHHEEC
IHDYDRIIQF
HHHHHHHHCC
>Mature Secondary Structure
MNYLIFVCAPPYSMQNSITAYMFAKSLFLLNYRVKKVFFYASGIYNANCMIPTEENEFNI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCEECCEEEEECCCCCHHH
LNEWINLKKKYNFKLCVCPSAAYKRGLLSNTHTSTLKLGYKYNFFSSSFQWMSLSELSYS
HHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEHHHHEEC
IHDYDRIIQF
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA