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The map label for this gene is yheM [H]

Identifier: 116515266

GI number: 116515266

Start: 374191

End: 374550

Strand: Reverse

Name: yheM [H]

Synonym: BCc_348

Alternate gene names: 116515266

Gene position: 374550-374191 (Counterclockwise)

Preceding gene: 116515267

Following gene: 116515265

Centisome position: 89.95

GC content: 19.72

Gene sequence:

>360_bases
ATGAAATCTATCGCATTTATTTTTTCAAATTCACCATATGGAAATTCTATTAGTAAAGAAGGTCTTGATGTTGTTCTTTC
ATGTTCTTTAACTACTAAAAAAATTGCACTATTTTTTATTGATGATGGTGTTTTACAATTATTTTTAAATCAAAAACCAG
AATATATTAATTCTTATAATTATTCTCTTTCTTTTAAAATTTTGTCATTATATGATATAAAAGATTTTTTTTTTTGTAAA
TATTCAGCATATAAACGTGGTTTAAAAGAAAATGATAATTTTTTATTACCGGTAAAATTTTTAAATAATATATGTTTGAG
AAGAAAAATTAATTCATTTGATTATATTTTGAAATGGTGA

Upstream 100 bases:

>100_bases
TCTTCTTCATTTCAATGGATGAGTTTAAGTGAATTATCTTATTCAATTCATGATTATGATCGTATTATACAGTTTTAAAT
TTTTTATTATATTAAATTTT

Downstream 100 bases:

>100_bases
TACACACATAATATGTTACATATTTTAATAAGTTCTCCATACTCTATCTCTATACCTTCATTATTAATGTTAAGTACTAC
ATTGGATGATTTAATTTGTA

Product: YheM

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein C [H]

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MKSIAFIFSNSPYGNSISKEGLDVVLSCSLTTKKIALFFIDDGVLQLFLNQKPEYINSYNYSLSFKILSLYDIKDFFFCK
YSAYKRGLKENDNFLLPVKFLNNICLRRKINSFDYILKW

Sequences:

>Translated_119_residues
MKSIAFIFSNSPYGNSISKEGLDVVLSCSLTTKKIALFFIDDGVLQLFLNQKPEYINSYNYSLSFKILSLYDIKDFFFCK
YSAYKRGLKENDNFLLPVKFLNNICLRRKINSFDYILKW
>Mature_119_residues
MKSIAFIFSNSPYGNSISKEGLDVVLSCSLTTKKIALFFIDDGVLQLFLNQKPEYINSYNYSLSFKILSLYDIKDFFFCK
YSAYKRGLKENDNFLLPVKFLNNICLRRKINSFDYILKW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions [H]

COG id: COG2923

COG function: function code P; Uncharacterized protein involved in the oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrF/tusC family [H]

Homologues:

Organism=Escherichia coli, GI1789742, Length=119, Percent_Identity=41.1764705882353, Blast_Score=108, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787
- InterPro:   IPR017462 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: NA

Molecular weight: Translated: 13960; Mature: 13960

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIAFIFSNSPYGNSISKEGLDVVLSCSLTTKKIALFFIDDGVLQLFLNQKPEYINSYN
CCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCHHCCCC
YSLSFKILSLYDIKDFFFCKYSAYKRGLKENDNFLLPVKFLNNICLRRKINSFDYILKW
EEEEEEEEEEECCCHHEEEEEHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MKSIAFIFSNSPYGNSISKEGLDVVLSCSLTTKKIALFFIDDGVLQLFLNQKPEYINSYN
CCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCEEEEECCCCCHHCCCC
YSLSFKILSLYDIKDFFFCKYSAYKRGLKENDNFLLPVKFLNNICLRRKINSFDYILKW
EEEEEEEEEEECCCHHEEEEEHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]