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The map label for this gene is greA

Identifier: 116515169

GI number: 116515169

Start: 274479

End: 274958

Strand: Reverse

Name: greA

Synonym: BCc_237

Alternate gene names: 116515169

Gene position: 274958-274479 (Counterclockwise)

Preceding gene: 116515174

Following gene: 116515168

Centisome position: 66.04

GC content: 23.33

Gene sequence:

>480_bases
TTGTTTAATAAAATTCCAATGACATTACGGGGTTTTAATAAATTAAAATCTGAATTAAAAAAATTAAAATACATTACTAG
ACCTTGTATTGTAAAAGCAATTGCAAACGCTCGTCAATTAGGTGATTTAAAAGAAAATGCAGAGTATCATTCAGCACGTG
AAGAACAGAGTTTTTGTGAAAATAGAATTTGTGAAATTGAAAATAAATTATTACGTGCTCAAATAATTGATATTACTAAA
ATACCTTTTAAAGGTATTGTTATTTTTGGATCTACAGTAACTGTATTGCAAATTAGTACTAGTAATATTTTTGTATATAC
TATTGTTGGTGACGATGAAGCAAATTATAAAGAATTTTCAATTTCTATTCATTCACCTATGTCAAGAGCTTTAATTGGAA
AAAAAAAAAATGATATTATAAAAGTTAATACACCTACAGGTTATATAAAATATATTATTAAAAAAATTGAATATATTTAA

Upstream 100 bases:

>100_bases
TTTTAATAAAAATTTATTATTATTTATTTTTAAATATGAATTATTATATTATAATAAATAATATTTATATATAAAATTTT
TTGATAAAGGAGTTATAAGC

Downstream 100 bases:

>100_bases
TTAAAAATATTGTTTATATAAAAATTTTTTTTTGAAATTAAAAATTTAATATATAAAAAATCTTATTTTAATTTTAGAAA
TTTGTTTTTTTATGTATAAT

Product: hypothetical protein

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MFNKIPMTLRGFNKLKSELKKLKYITRPCIVKAIANARQLGDLKENAEYHSAREEQSFCENRICEIENKLLRAQIIDITK
IPFKGIVIFGSTVTVLQISTSNIFVYTIVGDDEANYKEFSISIHSPMSRALIGKKKNDIIKVNTPTGYIKYIIKKIEYI

Sequences:

>Translated_159_residues
MFNKIPMTLRGFNKLKSELKKLKYITRPCIVKAIANARQLGDLKENAEYHSAREEQSFCENRICEIENKLLRAQIIDITK
IPFKGIVIFGSTVTVLQISTSNIFVYTIVGDDEANYKEFSISIHSPMSRALIGKKKNDIIKVNTPTGYIKYIIKKIEYI
>Mature_159_residues
MFNKIPMTLRGFNKLKSELKKLKYITRPCIVKAIANARQLGDLKENAEYHSAREEQSFCENRICEIENKLLRAQIIDITK
IPFKGIVIFGSTVTVLQISTSNIFVYTIVGDDEANYKEFSISIHSPMSRALIGKKKNDIIKVNTPTGYIKYIIKKIEYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=155, Percent_Identity=55.4838709677419, Blast_Score=186, Evalue=7e-49,
Organism=Escherichia coli, GI87082261, Length=157, Percent_Identity=34.3949044585987, Blast_Score=80, Evalue=4e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_BUCCC (Q057J4)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802798.1
- ProteinModelPortal:   Q057J4
- SMR:   Q057J4
- STRING:   Q057J4
- EnsemblBacteria:   EBBUCT00000001549
- GeneID:   4440696
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_237
- eggNOG:   COG0782
- GeneTree:   EBGT00050000007852
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- BioCyc:   BAPH372461:BCC_237-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18235; Mature: 18235

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKIPMTLRGFNKLKSELKKLKYITRPCIVKAIANARQLGDLKENAEYHSAREEQSFCE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHH
NRICEIENKLLRAQIIDITKIPFKGIVIFGSTVTVLQISTSNIFVYTIVGDDEANYKEFS
HHHHHHHHHHHHHHHHHEECCCCCEEEEECCEEEEEEEECCCEEEEEEECCCCCCCEEEE
ISIHSPMSRALIGKKKNDIIKVNTPTGYIKYIIKKIEYI
EEECCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFNKIPMTLRGFNKLKSELKKLKYITRPCIVKAIANARQLGDLKENAEYHSAREEQSFCE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHH
NRICEIENKLLRAQIIDITKIPFKGIVIFGSTVTVLQISTSNIFVYTIVGDDEANYKEFS
HHHHHHHHHHHHHHHHHEECCCCCEEEEECCEEEEEEEECCCEEEEEEECCCCCCCEEEE
ISIHSPMSRALIGKKKNDIIKVNTPTGYIKYIIKKIEYI
EEECCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA