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The map label for this gene is yfgB

Identifier: 116515112

GI number: 116515112

Start: 204572

End: 205651

Strand: Direct

Name: yfgB

Synonym: BCc_176

Alternate gene names: 116515112

Gene position: 204572-205651 (Clockwise)

Preceding gene: 116515110

Following gene: 116515113

Centisome position: 49.13

GC content: 18.98

Gene sequence:

>1080_bases
ATGTGTAAAAAAAATATTTTATCAAATATTAAAAAAAAAAAAATTAATTTATTAAATTTTAATTTAAAAAAAATGATAAA
TTTTTTTATAAAAATAGGAGAAAAAAAATTTCGAGCTATTCAAATCACTGATTGGATTTACAAAAAACAAAATATAAAAT
TTGATCAAATGAGTAATTTAAATTTTTTTTTAAAAAAAAAATTAAATAATATAGCTGTTATTAAAATTCCAAAATGTATA
AAAAAAATAAAATCGATAGATGGCACTATTAAATGGAAATTTTTATGTAATAAAGAATTTATTGAAACTATATATATTCC
AGAAAAAAAACGTGCTACTTTGTGTATTTCTTCACAAGTAGGATGTCAATTAAAATGTAATTTTTGCGCTACTGGTCAAT
TAGGATATAAAAGAAATTTATTGGTTTCAGAAATTATTGGTCAAATTTGGTATGTTATAAATAAAATAAAAAAATATAAT
AGCAAAAAAAAAAATTTTCCTCCTATTAAAAATATTGTTATGATGGGTATGGGAGAACCGTTATTAAATTTAAAAAATAT
AATAATAGCAATAGATATTATATTAGGAAATTATGGTTTTAATTTTTCTAAAAATAAAGTAACTTTATCGACTTCTGGTA
TTGTTCCCGCTATTAATAAAATAGCTGGAAAAATTGATATTTCTTTAGCAGTTTCTTTACATGCATCTAATAATACTATA
CGTAATAAAATTATGCCAATAAATAAAATTTATAATATTCAATTATTATTAGAATCTATAAAAAATTATTTAAAAAAATC
TTCTGCAAATAAAGGAATAGTAACTATAGAATATGTTATGTTATCAAAAATTAATGATTTTCAACATCATGCAATTGAAT
TATCAAATTTATTAAAAAATATACCATGTAAAATTAATTTAATTCCATGGAATCCTATTAAAAATTCTTCTTATATTTGT
AGTAGTAGTAAAAATATTATAAATTTTGCTAATTTTTTAAGAAAAAAAGGTTTTATTGTGATTATTAGAAAAAATAGAGG
TTCTGATATTCAAGCAGCATGTGGGCAATTAATAGCTTAA

Upstream 100 bases:

>100_bases
ATTTTTATAAATAAAAGTTTACCTTTATTTTAAAAGATAAATTTTATATCTATTTTTATAAAACTATTTCTAAAGATAAT
TATATATATTGGAAATAAAT

Downstream 100 bases:

>100_bases
TATTATTTTAAATAAGTAATATTTATTTATAAATATTAAGTACACAAATAATAATAGTATTAATATAGGAAATTATAAAA
TATGAATGTGTCATATCGAT

Product: YfgB

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MCKKNILSNIKKKKINLLNFNLKKMINFFIKIGEKKFRAIQITDWIYKKQNIKFDQMSNLNFFLKKKLNNIAVIKIPKCI
KKIKSIDGTIKWKFLCNKEFIETIYIPEKKRATLCISSQVGCQLKCNFCATGQLGYKRNLLVSEIIGQIWYVINKIKKYN
SKKKNFPPIKNIVMMGMGEPLLNLKNIIIAIDIILGNYGFNFSKNKVTLSTSGIVPAINKIAGKIDISLAVSLHASNNTI
RNKIMPINKIYNIQLLLESIKNYLKKSSANKGIVTIEYVMLSKINDFQHHAIELSNLLKNIPCKINLIPWNPIKNSSYIC
SSSKNIINFANFLRKKGFIVIIRKNRGSDIQAACGQLIA

Sequences:

>Translated_359_residues
MCKKNILSNIKKKKINLLNFNLKKMINFFIKIGEKKFRAIQITDWIYKKQNIKFDQMSNLNFFLKKKLNNIAVIKIPKCI
KKIKSIDGTIKWKFLCNKEFIETIYIPEKKRATLCISSQVGCQLKCNFCATGQLGYKRNLLVSEIIGQIWYVINKIKKYN
SKKKNFPPIKNIVMMGMGEPLLNLKNIIIAIDIILGNYGFNFSKNKVTLSTSGIVPAINKIAGKIDISLAVSLHASNNTI
RNKIMPINKIYNIQLLLESIKNYLKKSSANKGIVTIEYVMLSKINDFQHHAIELSNLLKNIPCKINLIPWNPIKNSSYIC
SSSKNIINFANFLRKKGFIVIIRKNRGSDIQAACGQLIA
>Mature_359_residues
MCKKNILSNIKKKKINLLNFNLKKMINFFIKIGEKKFRAIQITDWIYKKQNIKFDQMSNLNFFLKKKLNNIAVIKIPKCI
KKIKSIDGTIKWKFLCNKEFIETIYIPEKKRATLCISSQVGCQLKCNFCATGQLGYKRNLLVSEIIGQIWYVINKIKKYN
SKKKNFPPIKNIVMMGMGEPLLNLKNIIIAIDIILGNYGFNFSKNKVTLSTSGIVPAINKIAGKIDISLAVSLHASNNTI
RNKIMPINKIYNIQLLLESIKNYLKKSSANKGIVTIEYVMLSKINDFQHHAIELSNLLKNIPCKINLIPWNPIKNSSYIC
SSSKNIINFANFLRKKGFIVIIRKNRGSDIQAACGQLIA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=347, Percent_Identity=52.1613832853026, Blast_Score=393, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BUCCC (Q057Q1)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802741.1
- ProteinModelPortal:   Q057Q1
- STRING:   Q057Q1
- EnsemblBacteria:   EBBUCT00000001269
- GeneID:   4440787
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_176
- eggNOG:   COG0820
- GeneTree:   EBGT00050000007926
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- BioCyc:   BAPH372461:BCC_176-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41039; Mature: 41039

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKKNILSNIKKKKINLLNFNLKKMINFFIKIGEKKFRAIQITDWIYKKQNIKFDQMSNL
CCCHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCCHHHCCCC
NFFLKKKLNNIAVIKIPKCIKKIKSIDGTIKWKFLCNKEFIETIYIPEKKRATLCISSQV
HHHHHHHCCCEEEEECHHHHHHHHCCCCEEEEEEEECHHHHHEEECCCCCCEEEEEECCC
GCQLKCNFCATGQLGYKRNLLVSEIIGQIWYVINKIKKYNSKKKNFPPIKNIVMMGMGEP
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
LLNLKNIIIAIDIILGNYGFNFSKNKVTLSTSGIVPAINKIAGKIDISLAVSLHASNNTI
HHHHHHEEEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHCCEEEEEEEEEEECCCCCH
RNKIMPINKIYNIQLLLESIKNYLKKSSANKGIVTIEYVMLSKINDFQHHAIELSNLLKN
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHCCHHHHHHHHHHHHHHC
IPCKINLIPWNPIKNSSYICSSSKNIINFANFLRKKGFIVIIRKNRGSDIQAACGQLIA
CCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MCKKNILSNIKKKKINLLNFNLKKMINFFIKIGEKKFRAIQITDWIYKKQNIKFDQMSNL
CCCHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCCHHHCCCC
NFFLKKKLNNIAVIKIPKCIKKIKSIDGTIKWKFLCNKEFIETIYIPEKKRATLCISSQV
HHHHHHHCCCEEEEECHHHHHHHHCCCCEEEEEEEECHHHHHEEECCCCCCEEEEEECCC
GCQLKCNFCATGQLGYKRNLLVSEIIGQIWYVINKIKKYNSKKKNFPPIKNIVMMGMGEP
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
LLNLKNIIIAIDIILGNYGFNFSKNKVTLSTSGIVPAINKIAGKIDISLAVSLHASNNTI
HHHHHHEEEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHCCEEEEEEEEEEECCCCCH
RNKIMPINKIYNIQLLLESIKNYLKKSSANKGIVTIEYVMLSKINDFQHHAIELSNLLKN
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHCCHHHHHHHHHHHHHHC
IPCKINLIPWNPIKNSSYICSSSKNIINFANFLRKKGFIVIIRKNRGSDIQAACGQLIA
CCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA