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The map label for this gene is truA [H]

Identifier: 116515069

GI number: 116515069

Start: 148324

End: 149133

Strand: Direct

Name: truA [H]

Synonym: BCc_129

Alternate gene names: 116515069

Gene position: 148324-149133 (Clockwise)

Preceding gene: 116515064

Following gene: 116515070

Centisome position: 35.62

GC content: 22.35

Gene sequence:

>810_bases
ATGAAGTTCGTTTTTGGTGTAGAATATGATGGTAGTATATATCACGGGTGGCAAAAACAAAAATCAGTATTAACAATACA
AGAATGTATTGAACATGCTTTATCTAAAATTGCTAATTCTAAAATAGAAGTAATATGTGCTGGAAGAACAGATAAAGGTG
TACATGCAATTACTCAAATAGTCCATTTTTCTTCTCTTATAATTCGTAATGAAGAAGTTTGGTTTACAGGATTAAATTCA
TTATTGCCAAAAAATATAACTGTTTTATGGTTAAAAAAAGTTTCATCAAATTTTCATGCTAGATTTTCTGCATTGTCTAG
ATCATATAGATATATAATTTTTAATAGAAAAAATCGATCAAGTTTTTTAAAAAACTATTATTTTCATGTAAGAAATTTTT
TAGATATTTATAAAATGAAAACTAGTTCTCAATTTTTAATTGGAAATCATGATTTTTCTTCTTTTAAAAGTAGTGGTTGT
CAATCATTTTCTTCAAGAAAAAATATATTTTCTTTAATAATATATCGGATAAATGATTTTATAATTATTGATATTACTGC
AAATTCTTTTTTATATCATATGGTAAGAAATATTGTAGGTTGTTTAATTTTAGTAGGTTTATCTAAAAAAAAACCAGAAT
GGGTGCAATATATATTACAAAAAAAAGATATTAAAAAAACATATTCTACTGTTCCTGCACAGGGATTATATTTTTTATTT
GCTTCATATCCAGATTGTTTTTCTATATTAAATCAATCTTTTTTATTAAAAAAAAATAATTTATTTACTCATTTTTTTAG
TAACTTTTAA

Upstream 100 bases:

>100_bases
TGTTATAATGAAAATATTTATTTTTAATAAATATTGTTTTTAAAGATTTTTTTATTTTTTTTTTATCAATTTTTTTAAAT
ATTTTTATTAGGAAATATAA

Downstream 100 bases:

>100_bases
TTATTATAAAATTTTTAAAAATATTTAAATATTTATTTAAATGTTTATTTAAATATTAATAATTTATTAAAATATAAATA
TTTTTATAATATAATTACAA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKFVFGVEYDGSIYHGWQKQKSVLTIQECIEHALSKIANSKIEVICAGRTDKGVHAITQIVHFSSLIIRNEEVWFTGLNS
LLPKNITVLWLKKVSSNFHARFSALSRSYRYIIFNRKNRSSFLKNYYFHVRNFLDIYKMKTSSQFLIGNHDFSSFKSSGC
QSFSSRKNIFSLIIYRINDFIIIDITANSFLYHMVRNIVGCLILVGLSKKKPEWVQYILQKKDIKKTYSTVPAQGLYFLF
ASYPDCFSILNQSFLLKKNNLFTHFFSNF

Sequences:

>Translated_269_residues
MKFVFGVEYDGSIYHGWQKQKSVLTIQECIEHALSKIANSKIEVICAGRTDKGVHAITQIVHFSSLIIRNEEVWFTGLNS
LLPKNITVLWLKKVSSNFHARFSALSRSYRYIIFNRKNRSSFLKNYYFHVRNFLDIYKMKTSSQFLIGNHDFSSFKSSGC
QSFSSRKNIFSLIIYRINDFIIIDITANSFLYHMVRNIVGCLILVGLSKKKPEWVQYILQKKDIKKTYSTVPAQGLYFLF
ASYPDCFSILNQSFLLKKNNLFTHFFSNF
>Mature_269_residues
MKFVFGVEYDGSIYHGWQKQKSVLTIQECIEHALSKIANSKIEVICAGRTDKGVHAITQIVHFSSLIIRNEEVWFTGLNS
LLPKNITVLWLKKVSSNFHARFSALSRSYRYIIFNRKNRSSFLKNYYFHVRNFLDIYKMKTSSQFLIGNHDFSSFKSSGC
QSFSSRKNIFSLIIYRINDFIIIDITANSFLYHMVRNIVGCLILVGLSKKKPEWVQYILQKKDIKKTYSTVPAQGLYFLF
ASYPDCFSILNQSFLLKKNNLFTHFFSNF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=261, Percent_Identity=27.9693486590038, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI199559785, Length=287, Percent_Identity=27.1777003484321, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=43.1451612903226, Blast_Score=245, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71985046, Length=292, Percent_Identity=26.3698630136986, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321112, Length=222, Percent_Identity=32.8828828828829, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=27.3927392739274, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31399; Mature: 31399

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVFGVEYDGSIYHGWQKQKSVLTIQECIEHALSKIANSKIEVICAGRTDKGVHAITQI
CCEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
VHFSSLIIRNEEVWFTGLNSLLPKNITVLWLKKVSSNFHARFSALSRSYRYIIFNRKNRS
HHHHHHHEECCCEEEECHHHHCCCCEEEEEEEHHCCCHHHHHHHHCCCEEEEEEECCCHH
SFLKNYYFHVRNFLDIYKMKTSSQFLIGNHDFSSFKSSGCQSFSSRKNIFSLIIYRINDF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEECCE
IIIDITANSFLYHMVRNIVGCLILVGLSKKKPEWVQYILQKKDIKKTYSTVPAQGLYFLF
EEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEE
ASYPDCFSILNQSFLLKKNNLFTHFFSNF
CCCCHHHHHHCHHHEEECCCHHHHHHHCC
>Mature Secondary Structure
MKFVFGVEYDGSIYHGWQKQKSVLTIQECIEHALSKIANSKIEVICAGRTDKGVHAITQI
CCEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
VHFSSLIIRNEEVWFTGLNSLLPKNITVLWLKKVSSNFHARFSALSRSYRYIIFNRKNRS
HHHHHHHEECCCEEEECHHHHCCCCEEEEEEEHHCCCHHHHHHHHCCCEEEEEEECCCHH
SFLKNYYFHVRNFLDIYKMKTSSQFLIGNHDFSSFKSSGCQSFSSRKNIFSLIIYRINDF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEECCE
IIIDITANSFLYHMVRNIVGCLILVGLSKKKPEWVQYILQKKDIKKTYSTVPAQGLYFLF
EEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEE
ASYPDCFSILNQSFLLKKNNLFTHFFSNF
CCCCHHHHHHCHHHEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA