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The map label for this gene is carA [H]

Identifier: 116515032

GI number: 116515032

Start: 101834

End: 102964

Strand: Reverse

Name: carA [H]

Synonym: BCc_091

Alternate gene names: 116515032

Gene position: 102964-101834 (Counterclockwise)

Preceding gene: 116515033

Following gene: 116515031

Centisome position: 24.73

GC content: 22.55

Gene sequence:

>1131_bases
TTGACAAAATTAGCGAATTTAATTTTAGAAAATGGTACTATTTTCTCAGGTATTAATGTTGGAATTGAAGGAGAAATAGT
TGGAGAAATTGTATTTAATACAGCTATAACCGGATATCAAGAAATATTAACTGATCCATCATATTTAGGACAAATTATCG
TATTTACATATCCACATATAGGAAATGTTGGTACTAATACACAAGACGAGGAATCCAAAAAAATTCATGCTAATGGAATT
ATTATGAGATCTATATCAAAAATAGATAGTAATTTTCGCAGTACAAAGAATATACTTCAATATATTAAAAAAAATAAAAT
TATTGCTATATCTGATATTGATACTAGAAAATTAACACATATCATCCGAAATAATGGATCACAGTATGGATGTATTCAAT
CTGGAAAATATATTAATATTAGATATGCTTTTAAAAAAATCAAAAAATATAAAAAAAAAAGCAAAGAAAAAAAAATAAAA
TATCTAACAACAAAAAAAATAAAAATATGGAATAATAAAAATTATTTTTCAAAAAAAAAAACTTTTCATGTTGTTGTATA
TAACTTTGGTGTAAAAAAAAGTATATTAAAAATATTAGATAAAATGGGATGTAAAATTACTCTAGTATCATATAACATTA
ATATAAAAAAAATTATATCATTAAATCCTTCTGGAATTCTATTATCCAACGGACCAGGCGATCCCCGATCATACATTGTA
GCTATTAAAAACGTTAAAAAATTATTAAATTTTTCTATTCCTATATTTGGAATTTGTTTAGGACATCAAATTTTAGCTTT
ATCATTAGGATCTAAAATAATAAAAATGAAATTTGGACATCACGGGTCTAATCATCCAGTACAAAATATATTAACAAAAC
GTGTATTTATTACTTCTCAAAATCATAATTTTACTATAGATCCTAAAACTATAAAAAAAAATATGTCAGTAACACATATA
TCTTTATTTGATAAAACTATTCAAGGAATTTCATTAAAAAATCACTTATCTTTTAGTTTCCAAGGACATCCAGAATCAAA
TCCTGGAACTCATGATATTAAAAAATTATTTAATAAATTTATTAAAAATATGAAATATAAGGAAAAAAAATGCCAAAAAG
AGAAGATATAA

Upstream 100 bases:

>100_bases
AAAAAAATTAAAATATAAAAAAGAATACATATTCTATATTATAAAATAATATACATTCTTAATATTAATAATTTTTTATA
AAATAAATTAGGAAAAAATT

Downstream 100 bases:

>100_bases
AAAAAATATTAATAATTGGTGCTGGCCCAATAATCATTGGACAAGCATGTGAATTTGATTATTCAGGAGCTCAAGCTTGT
AAAGCTTTAAAAGAAGAAGG

Product: hypothetical protein

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTKLANLILENGTIFSGINVGIEGEIVGEIVFNTAITGYQEILTDPSYLGQIIVFTYPHIGNVGTNTQDEESKKIHANGI
IMRSISKIDSNFRSTKNILQYIKKNKIIAISDIDTRKLTHIIRNNGSQYGCIQSGKYINIRYAFKKIKKYKKKSKEKKIK
YLTTKKIKIWNNKNYFSKKKTFHVVVYNFGVKKSILKILDKMGCKITLVSYNINIKKIISLNPSGILLSNGPGDPRSYIV
AIKNVKKLLNFSIPIFGICLGHQILALSLGSKIIKMKFGHHGSNHPVQNILTKRVFITSQNHNFTIDPKTIKKNMSVTHI
SLFDKTIQGISLKNHLSFSFQGHPESNPGTHDIKKLFNKFIKNMKYKEKKCQKEKI

Sequences:

>Translated_376_residues
MTKLANLILENGTIFSGINVGIEGEIVGEIVFNTAITGYQEILTDPSYLGQIIVFTYPHIGNVGTNTQDEESKKIHANGI
IMRSISKIDSNFRSTKNILQYIKKNKIIAISDIDTRKLTHIIRNNGSQYGCIQSGKYINIRYAFKKIKKYKKKSKEKKIK
YLTTKKIKIWNNKNYFSKKKTFHVVVYNFGVKKSILKILDKMGCKITLVSYNINIKKIISLNPSGILLSNGPGDPRSYIV
AIKNVKKLLNFSIPIFGICLGHQILALSLGSKIIKMKFGHHGSNHPVQNILTKRVFITSQNHNFTIDPKTIKKNMSVTHI
SLFDKTIQGISLKNHLSFSFQGHPESNPGTHDIKKLFNKFIKNMKYKEKKCQKEKI
>Mature_375_residues
TKLANLILENGTIFSGINVGIEGEIVGEIVFNTAITGYQEILTDPSYLGQIIVFTYPHIGNVGTNTQDEESKKIHANGII
MRSISKIDSNFRSTKNILQYIKKNKIIAISDIDTRKLTHIIRNNGSQYGCIQSGKYINIRYAFKKIKKYKKKSKEKKIKY
LTTKKIKIWNNKNYFSKKKTFHVVVYNFGVKKSILKILDKMGCKITLVSYNINIKKIISLNPSGILLSNGPGDPRSYIVA
IKNVKKLLNFSIPIFGICLGHQILALSLGSKIIKMKFGHHGSNHPVQNILTKRVFITSQNHNFTIDPKTIKKNMSVTHIS
LFDKTIQGISLKNHLSFSFQGHPESNPGTHDIKKLFNKFIKNMKYKEKKCQKEKI

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI21361331, Length=376, Percent_Identity=33.5106382978723, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI169790915, Length=376, Percent_Identity=33.5106382978723, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI18105007, Length=374, Percent_Identity=30.7486631016043, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1786215, Length=372, Percent_Identity=48.6559139784946, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI193204318, Length=377, Percent_Identity=32.0954907161804, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=35.5670103092784, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=31.0432569974555, Blast_Score=192, Evalue=5e-50,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=31.6062176165803, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=31.6062176165803, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42748; Mature: 42617

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLANLILENGTIFSGINVGIEGEIVGEIVFNTAITGYQEILTDPSYLGQIIVFTYPHI
CCHHHHHHHCCCCEEECEECCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCCC
GNVGTNTQDEESKKIHANGIIMRSISKIDSNFRSTKNILQYIKKNKIIAISDIDTRKLTH
CCCCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHH
IIRNNGSQYGCIQSGKYINIRYAFKKIKKYKKKSKEKKIKYLTTKKIKIWNNKNYFSKKK
HHHCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCC
TFHVVVYNFGVKKSILKILDKMGCKITLVSYNINIKKIISLNPSGILLSNGPGDPRSYIV
EEEEEEEECCCHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCEEEEECCCCCCHHHEE
AIKNVKKLLNFSIPIFGICLGHQILALSLGSKIIKMKFGHHGSNHPVQNILTKRVFITSQ
EHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHEEEEEEC
NHNFTIDPKTIKKNMSVTHISLFDKTIQGISLKNHLSFSFQGHPESNPGTHDIKKLFNKF
CCCEEECHHHHHCCCCEEEEEHHHHHHHCCEEECCEEEEECCCCCCCCCHHHHHHHHHHH
IKNMKYKEKKCQKEKI
HHCCCHHHHHHHHCCC
>Mature Secondary Structure 
TKLANLILENGTIFSGINVGIEGEIVGEIVFNTAITGYQEILTDPSYLGQIIVFTYPHI
CHHHHHHHCCCCEEECEECCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCCC
GNVGTNTQDEESKKIHANGIIMRSISKIDSNFRSTKNILQYIKKNKIIAISDIDTRKLTH
CCCCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHH
IIRNNGSQYGCIQSGKYINIRYAFKKIKKYKKKSKEKKIKYLTTKKIKIWNNKNYFSKKK
HHHCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCC
TFHVVVYNFGVKKSILKILDKMGCKITLVSYNINIKKIISLNPSGILLSNGPGDPRSYIV
EEEEEEEECCCHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCEEEEECCCCCCHHHEE
AIKNVKKLLNFSIPIFGICLGHQILALSLGSKIIKMKFGHHGSNHPVQNILTKRVFITSQ
EHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHEEEEEEC
NHNFTIDPKTIKKNMSVTHISLFDKTIQGISLKNHLSFSFQGHPESNPGTHDIKKLFNKF
CCCEEECHHHHHCCCCEEEEEHHHHHHHCCEEECCEEEEECCCCCCCCCHHHHHHHHHHH
IKNMKYKEKKCQKEKI
HHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]