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The map label for this gene is rmlB [H]

Identifier: 116327948

GI number: 116327948

Start: 1455424

End: 1456473

Strand: Direct

Name: rmlB [H]

Synonym: LBL_1237

Alternate gene names: 116327948

Gene position: 1455424-1456473 (Clockwise)

Preceding gene: 116327947

Following gene: 116327949

Centisome position: 40.27

GC content: 38.0

Gene sequence:

>1050_bases
ATGAAAAAAATTTTAGTCACCGGTGGAGCAGGATTTATAGGTTCCAATTTTGTGAATCTCATTTTAAACGAAAATGAAGA
ATATCAGATAATCGTATTCGATAAACTGACTTATGCTGGAAATCTGAAAAGTTTGGAATATTGGAAAAAAGATCCTAGGT
TTATTTTTATAAAGGCGGACATCGCCAATAAAGAAAATGTATTTTCTATTTTCCAAGAACATAACTTCGATTACGTGGCG
CACTTCGCTGCGGAAAGCCACGTGGATCGTTCTATTTTGGGTCCGGAAGAATTCATCAAAACGAACGTACTCGGTACTTT
TTATCTTTTAGATGCGGCTAGGTTACAATGGAATGGATCTTATGAAGGAAAAAAATTCCTTCACGTATCCACAGACGAAG
TATTCGGAACATTAGGTGATACTGGATATTTCACCGAAGAGACCCCTTATGCGCCTAACTCTCCTTATTCGGCGTCTAAA
GCCGGTTCGGATCATATCGTAAGATCCTATTATCATACTTATCATATGCCAGTAGTGACCACAAATTGTTCCAATAACTA
TGGACCTTATCATTTTCCGGAAAAATTGATTCCTTTGACAATTTTAAATTGTTTACAGGGAAAACCTCTTCCCGTTTACG
GAGATGGAAAGAATATTAGGGATTGGTTATATGTTAAGGATCACTGCGAAGCATTGCGTCTTGCTTTATTCCAGGGATTG
CCGGGAGAAACTTATAATATAGGGACTCGAAACGAAAAGAAGAATATAGATATAGTAAATTCGATCTGTTCGATCATGGA
CGAGTTTCATCCTTCCGGCGTTCCGCATTCTAAATTGATTCAATATGTAAAGGACAGGCCTGGACACGATTTTCGTTACG
CAATTGATCCTTCCAAAATTGAAAAAGAACTGGGCTGGAAACCAAAGTTTGTGTTCGAATCAGCTCTCAGAGAGACGGTC
CGGTGGTATTTAGACAACGAGTCTTGGTGGAAGGAAATTCTTTCCGGTCAATATAAAGAATATTACCAAAATCAGTATGA
GAAACATTAG

Upstream 100 bases:

>100_bases
ATTTAAATGAAACTCGAAAAATTTTTGGTCCCGTTCCGCATTGGAAAGAAGATTTAACTCTCTGTCTGAAAGAAATCGCC
GAGATTTTCGGAAAAAAAGT

Downstream 100 bases:

>100_bases
ATGAAATCGAGAAAAGGGATTATACTCGCAGGCGGTTCTGGAACAAGGCTTTATCCGGTTACGTACGTCGTTTCCAAACA
ACTTTTGCCGGTTTACGATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKKILVTGGAGFIGSNFVNLILNENEEYQIIVFDKLTYAGNLKSLEYWKKDPRFIFIKADIANKENVFSIFQEHNFDYVA
HFAAESHVDRSILGPEEFIKTNVLGTFYLLDAARLQWNGSYEGKKFLHVSTDEVFGTLGDTGYFTEETPYAPNSPYSASK
AGSDHIVRSYYHTYHMPVVTTNCSNNYGPYHFPEKLIPLTILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRLALFQGL
PGETYNIGTRNEKKNIDIVNSICSIMDEFHPSGVPHSKLIQYVKDRPGHDFRYAIDPSKIEKELGWKPKFVFESALRETV
RWYLDNESWWKEILSGQYKEYYQNQYEKH

Sequences:

>Translated_349_residues
MKKILVTGGAGFIGSNFVNLILNENEEYQIIVFDKLTYAGNLKSLEYWKKDPRFIFIKADIANKENVFSIFQEHNFDYVA
HFAAESHVDRSILGPEEFIKTNVLGTFYLLDAARLQWNGSYEGKKFLHVSTDEVFGTLGDTGYFTEETPYAPNSPYSASK
AGSDHIVRSYYHTYHMPVVTTNCSNNYGPYHFPEKLIPLTILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRLALFQGL
PGETYNIGTRNEKKNIDIVNSICSIMDEFHPSGVPHSKLIQYVKDRPGHDFRYAIDPSKIEKELGWKPKFVFESALRETV
RWYLDNESWWKEILSGQYKEYYQNQYEKH
>Mature_349_residues
MKKILVTGGAGFIGSNFVNLILNENEEYQIIVFDKLTYAGNLKSLEYWKKDPRFIFIKADIANKENVFSIFQEHNFDYVA
HFAAESHVDRSILGPEEFIKTNVLGTFYLLDAARLQWNGSYEGKKFLHVSTDEVFGTLGDTGYFTEETPYAPNSPYSASK
AGSDHIVRSYYHTYHMPVVTTNCSNNYGPYHFPEKLIPLTILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRLALFQGL
PGETYNIGTRNEKKNIDIVNSICSIMDEFHPSGVPHSKLIQYVKDRPGHDFRYAIDPSKIEKELGWKPKFVFESALRETV
RWYLDNESWWKEILSGQYKEYYQNQYEKH

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=38.2530120481928, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=25.609756097561, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI56237023, Length=357, Percent_Identity=24.0896358543417, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI56118217, Length=357, Percent_Identity=24.0896358543417, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI189083684, Length=357, Percent_Identity=24.0896358543417, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4504031, Length=340, Percent_Identity=25.8823529411765, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=58.9080459770115, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=54.4692737430168, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=25.9259259259259, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=26.1494252873563, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=37.5384615384615, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=31.7073170731707, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=25.0755287009063, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=358, Percent_Identity=25.9776536312849, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=360, Percent_Identity=25.8333333333333, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=245, Percent_Identity=25.7142857142857, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=245, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=245, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=245, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=245, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=364, Percent_Identity=26.9230769230769, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356223, Length=328, Percent_Identity=27.7439024390244, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=29.2479108635098, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=25.9701492537313, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40413; Mature: 40413

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILVTGGAGFIGSNFVNLILNENEEYQIIVFDKLTYAGNLKSLEYWKKDPRFIFIKAD
CCEEEEECCCCCHHCCEEEEEECCCCCEEEEEEEEEECCCCCCHHHHHCCCCCEEEEEEC
IANKENVFSIFQEHNFDYVAHFAAESHVDRSILGPEEFIKTNVLGTFYLLDAARLQWNGS
CCCCHHHHHHHHHCCCCCEEHHHHHHCCCHHHCCHHHHHHHHHHHHHEEEEHHHEEECCC
YEGKKFLHVSTDEVFGTLGDTGYFTEETPYAPNSPYSASKAGSDHIVRSYYHTYHMPVVT
CCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEE
TNCSNNYGPYHFPEKLIPLTILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRLALFQGL
ECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEEHHHHHHHHHHHHHCC
PGETYNIGTRNEKKNIDIVNSICSIMDEFHPSGVPHSKLIQYVKDRPGHDFRYAIDPSKI
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECHHHH
EKELGWKPKFVFESALRETVRWYLDNESWWKEILSGQYKEYYQNQYEKH
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKILVTGGAGFIGSNFVNLILNENEEYQIIVFDKLTYAGNLKSLEYWKKDPRFIFIKAD
CCEEEEECCCCCHHCCEEEEEECCCCCEEEEEEEEEECCCCCCHHHHHCCCCCEEEEEEC
IANKENVFSIFQEHNFDYVAHFAAESHVDRSILGPEEFIKTNVLGTFYLLDAARLQWNGS
CCCCHHHHHHHHHCCCCCEEHHHHHHCCCHHHCCHHHHHHHHHHHHHEEEEHHHEEECCC
YEGKKFLHVSTDEVFGTLGDTGYFTEETPYAPNSPYSASKAGSDHIVRSYYHTYHMPVVT
CCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEE
TNCSNNYGPYHFPEKLIPLTILNCLQGKPLPVYGDGKNIRDWLYVKDHCEALRLALFQGL
ECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEEHHHHHHHHHHHHHCC
PGETYNIGTRNEKKNIDIVNSICSIMDEFHPSGVPHSKLIQYVKDRPGHDFRYAIDPSKI
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECHHHH
EKELGWKPKFVFESALRETVRWYLDNESWWKEILSGQYKEYYQNQYEKH
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]