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The map label for this gene is rfbD [C]

Identifier: 116327931

GI number: 116327931

Start: 1432453

End: 1433349

Strand: Direct

Name: rfbD [C]

Synonym: LBL_1217

Alternate gene names: 116327931

Gene position: 1432453-1433349 (Clockwise)

Preceding gene: 116327930

Following gene: 116327932

Centisome position: 39.63

GC content: 36.68

Gene sequence:

>897_bases
GTGTTATCCCCAAAAAAAATATTAGTTTTAGGCGTATCCGGAATGTTGGGAAACGCAGTGTTTCGCGTTCTATCCGAAAA
CGCCGAATTTGAAGTTCAAGGAACGATTAGAAGTATTGAATATTTGAGATTCTTTACGAAAGCGGAATCGGAAAGGATCA
CATCGAATATGGACGTTTTAAACGAGGACGAGTTGCTTCGTTTATTTTCAAACATAAAACCGGATATCGTTGTTAATTGT
GTGGGAATCATCAAACAGCAAAAATTGTCAAAGGATCCAATTACTGTTTTGCCCATCAATTCTCTTTTACCGCACAGACT
TTCCAATCTATGCAAATTAATCGGGGCCAGATTGATTTTGATCAGCACGGATTGTGTTTTTAACGGTCAAAAAGGGAATT
ATACGGAAACTGATACGCCCGATGCGGAAGATTTATACGGCAGATCCAAAGAAATCGGAGAAATCCGGGAAGAATCTCAT
GTGTTTACGGTACGCACTTCCATTATTGGCCATGAACTCAATTCTAACTATTCCTTGGTTGATTGGTTTTTATCACAAAA
CGGTGAAGTGAAAGGATACAAAAAGGCCTTTTTTTCGGGCCTTCCTTCCTGCGAAATCGCCGAAATCATTAAGACAGTAA
TCATTCCCAATCCGAAACTTTATGGACTCTATCATGTTTCTTCTGATCCTATTTCTAAATTCGATTTGCTGAGTTTAGTC
GGAGAAGTGTATGGAAAAAATAATAAAATTTTAGAAAGCGAAGAAGTAATTGTAGATCGCTCTTTGGATTCAACCAAATT
CAGAAAAGAAACTTCTTATAAACCTTTGCCATGGAAGAGTTTAATTTTTTTGATGAAAAAGTACAAAGAACAATATCTGG
ATAATATCCATGTTTAA

Upstream 100 bases:

>100_bases
TCATTTCGAAACGGGTTGGCCGAAATATTAAAAGTTCTCTGATTTTTAAACGAAACGTTTTTAAATGAAATCGATTGAAT
AAGGAAATCTGAGTCGTATC

Downstream 100 bases:

>100_bases
TAACAAAATACTGATGATTACCGGCGGAACCGGTTCTTTCGGGGATACGGTTTTAAAACGTTTTTTAGGAACCAATGTAA
AAGAAATACGTATCTTTAGC

Product: sugar oxidoreductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: Carbohydrate Oxidoreductase; NAD-Dependent Epimerase/Dehydratase; Reductase; Dtdp-4-Dehydrorhamnose Reductase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase; LPS Biosynthesis Related Reductase; TDP-4-Keto-L-Rhamnose Reductase.; DTDP-4-Keto-L-Rhamnose Reductase; Spore Coat Polysaccharide Biosynthesis Protein SpsK; Sugar Oxidoreductase; NAD Dependent Epimerase/Dehydratase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLSPKKILVLGVSGMLGNAVFRVLSENAEFEVQGTIRSIEYLRFFTKAESERITSNMDVLNEDELLRLFSNIKPDIVVNC
VGIIKQQKLSKDPITVLPINSLLPHRLSNLCKLIGARLILISTDCVFNGQKGNYTETDTPDAEDLYGRSKEIGEIREESH
VFTVRTSIIGHELNSNYSLVDWFLSQNGEVKGYKKAFFSGLPSCEIAEIIKTVIIPNPKLYGLYHVSSDPISKFDLLSLV
GEVYGKNNKILESEEVIVDRSLDSTKFRKETSYKPLPWKSLIFLMKKYKEQYLDNIHV

Sequences:

>Translated_298_residues
MLSPKKILVLGVSGMLGNAVFRVLSENAEFEVQGTIRSIEYLRFFTKAESERITSNMDVLNEDELLRLFSNIKPDIVVNC
VGIIKQQKLSKDPITVLPINSLLPHRLSNLCKLIGARLILISTDCVFNGQKGNYTETDTPDAEDLYGRSKEIGEIREESH
VFTVRTSIIGHELNSNYSLVDWFLSQNGEVKGYKKAFFSGLPSCEIAEIIKTVIIPNPKLYGLYHVSSDPISKFDLLSLV
GEVYGKNNKILESEEVIVDRSLDSTKFRKETSYKPLPWKSLIFLMKKYKEQYLDNIHV
>Mature_298_residues
MLSPKKILVLGVSGMLGNAVFRVLSENAEFEVQGTIRSIEYLRFFTKAESERITSNMDVLNEDELLRLFSNIKPDIVVNC
VGIIKQQKLSKDPITVLPINSLLPHRLSNLCKLIGARLILISTDCVFNGQKGNYTETDTPDAEDLYGRSKEIGEIREESH
VFTVRTSIIGHELNSNYSLVDWFLSQNGEVKGYKKAFFSGLPSCEIAEIIKTVIIPNPKLYGLYHVSSDPISKFDLLSLV
GEVYGKNNKILESEEVIVDRSLDSTKFRKETSYKPLPWKSLIFLMKKYKEQYLDNIHV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.133

Molecular weight: Translated: 33801; Mature: 33801

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPKKILVLGVSGMLGNAVFRVLSENAEFEVQGTIRSIEYLRFFTKAESERITSNMDVL
CCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
NEDELLRLFSNIKPDIVVNCVGIIKQQKLSKDPITVLPINSLLPHRLSNLCKLIGARLIL
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCEEEE
ISTDCVFNGQKGNYTETDTPDAEDLYGRSKEIGEIREESHVFTVRTSIIGHELNSNYSLV
EEECEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCEEEEEEHHHHHHHCCCCCCEE
DWFLSQNGEVKGYKKAFFSGLPSCEIAEIIKTVIIPNPKLYGLYHVSSDPISKFDLLSLV
HHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
GEVYGKNNKILESEEVIVDRSLDSTKFRKETSYKPLPWKSLIFLMKKYKEQYLDNIHV
HHHHCCCCCEECCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSPKKILVLGVSGMLGNAVFRVLSENAEFEVQGTIRSIEYLRFFTKAESERITSNMDVL
CCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
NEDELLRLFSNIKPDIVVNCVGIIKQQKLSKDPITVLPINSLLPHRLSNLCKLIGARLIL
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCEEEE
ISTDCVFNGQKGNYTETDTPDAEDLYGRSKEIGEIREESHVFTVRTSIIGHELNSNYSLV
EEECEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCEEEEEEHHHHHHHCCCCCCEE
DWFLSQNGEVKGYKKAFFSGLPSCEIAEIIKTVIIPNPKLYGLYHVSSDPISKFDLLSLV
HHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
GEVYGKNNKILESEEVIVDRSLDSTKFRKETSYKPLPWKSLIFLMKKYKEQYLDNIHV
HHHHCCCCCEECCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA