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The map label for this gene is hisF-2

Identifier: 116327926

GI number: 116327926

Start: 1427927

End: 1428691

Strand: Direct

Name: hisF-2

Synonym: LBL_1212

Alternate gene names: 116327926

Gene position: 1427927-1428691 (Clockwise)

Preceding gene: 116327925

Following gene: 116327927

Centisome position: 39.51

GC content: 40.39

Gene sequence:

>765_bases
ATGTTAAGACCCAGAATCATACCTGTGTTGTTGTTACAAGAAAACGGATTGGTCAAAACGATCCAATTCGGCGACGAAAG
ATATATCGGTGACCCATTGAATGCCGTAAGGATTTTTAATGAAAAAGAAGCGGATGAACTTGCGGTTTTAGATATTTCCG
CCAGCAAAAAAGGAAAGGAACCGAATTATCGTCTAATCGAACGTTTGGCGAATGAATGCAGAATGCCTTTGTGTTACGGC
GGTGGGATTAAGGATTTGGATCAGGCGAATCGAATTTTGAGTTTTGGTGTGGAAAAAATAATTGTAAGTTCACTCGCAAT
CGAAAATCCGAAAATGATTTCCACTATGGCCTCCTACTTGGGAAGTCAGAGTGTGGTCGTAGCAATTGATTTTAAAAAAG
CGATGTTATCGAGAAGATATGAAGTGATGATTCATAACGGGACTAAAAAAACTGGAAAACATCTCGAGGATTTAGTGAAA
GAAGTTATCGAATTGGGCGCGGGTGAAATCATACTCAATTCCATTGATCGCGACGGAACTATGGTCGGTTATGAGATCGA
GATCATCAAAAAGATTCAAAGCATCTGTAAGATTCCCATTACGGTTTTAGGTGGAGCGGGGTCGCTGGATCACATCAAAA
ATCTAATTCAGGAACTTGGAATTATCGGCGTAGCTGCGGGAAGTCTTTTTGTTTATAAAGGAGTACATAAGGCGGTTTTA
ATCAATTATCCGAACGGCACGGATAAAGAAGCACTATTTCCCTGA

Upstream 100 bases:

>100_bases
TGCAGTAAATCGCGAAAATATCTACGGAACTCAGTTTCATCCCGAAAAAAGTCATCACAATGGAGTCGCCTTAATTCGTA
ATTTTGCAAGTTTATAATTT

Downstream 100 bases:

>100_bases
AATAGGAAGCCAAATTTATTATGAAAGTTTTATATATTGCTCCTTTGCCGCCTCCGATCAACGGTCATTCCCTGGTTAGT
AAGGAATTTTATGACAGCAT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLRPRIIPVLLLQENGLVKTIQFGDERYIGDPLNAVRIFNEKEADELAVLDISASKKGKEPNYRLIERLANECRMPLCYG
GGIKDLDQANRILSFGVEKIIVSSLAIENPKMISTMASYLGSQSVVVAIDFKKAMLSRRYEVMIHNGTKKTGKHLEDLVK
EVIELGAGEIILNSIDRDGTMVGYEIEIIKKIQSICKIPITVLGGAGSLDHIKNLIQELGIIGVAAGSLFVYKGVHKAVL
INYPNGTDKEALFP

Sequences:

>Translated_254_residues
MLRPRIIPVLLLQENGLVKTIQFGDERYIGDPLNAVRIFNEKEADELAVLDISASKKGKEPNYRLIERLANECRMPLCYG
GGIKDLDQANRILSFGVEKIIVSSLAIENPKMISTMASYLGSQSVVVAIDFKKAMLSRRYEVMIHNGTKKTGKHLEDLVK
EVIELGAGEIILNSIDRDGTMVGYEIEIIKKIQSICKIPITVLGGAGSLDHIKNLIQELGIIGVAAGSLFVYKGVHKAVL
INYPNGTDKEALFP
>Mature_254_residues
MLRPRIIPVLLLQENGLVKTIQFGDERYIGDPLNAVRIFNEKEADELAVLDISASKKGKEPNYRLIERLANECRMPLCYG
GGIKDLDQANRILSFGVEKIIVSSLAIENPKMISTMASYLGSQSVVVAIDFKKAMLSRRYEVMIHNGTKKTGKHLEDLVK
EVIELGAGEIILNSIDRDGTMVGYEIEIIKKIQSICKIPITVLGGAGSLDHIKNLIQELGIIGVAAGSLFVYKGVHKAVL
INYPNGTDKEALFP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=244, Percent_Identity=36.0655737704918, Blast_Score=163, Evalue=9e-42,
Organism=Escherichia coli, GI87082028, Length=226, Percent_Identity=25.2212389380531, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=236, Percent_Identity=24.5762711864407, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_LEPBO (Q9ZGM0)

Other databases:

- EMBL:   AF078135
- ProteinModelPortal:   Q9ZGM0
- SMR:   Q9ZGM0
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27963; Mature: 27963

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPRIIPVLLLQENGLVKTIQFGDERYIGDPLNAVRIFNEKEADELAVLDISASKKGKE
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCHHHHEECCCCCCCCEEEEEECCCCCCCC
PNYRLIERLANECRMPLCYGGGIKDLDQANRILSFGVEKIIVSSLAIENPKMISTMASYL
CCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GSQSVVVAIDFKKAMLSRRYEVMIHNGTKKTGKHLEDLVKEVIELGAGEIILNSIDRDGT
CCCCEEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
MVGYEIEIIKKIQSICKIPITVLGGAGSLDHIKNLIQELGIIGVAAGSLFVYKGVHKAVL
EEEHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEECCCEEEE
INYPNGTDKEALFP
EECCCCCCCCCCCC
>Mature Secondary Structure
MLRPRIIPVLLLQENGLVKTIQFGDERYIGDPLNAVRIFNEKEADELAVLDISASKKGKE
CCCCCEEEEEEEECCCCEEEEEECCCCCCCCCHHHHEECCCCCCCCEEEEEECCCCCCCC
PNYRLIERLANECRMPLCYGGGIKDLDQANRILSFGVEKIIVSSLAIENPKMISTMASYL
CCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GSQSVVVAIDFKKAMLSRRYEVMIHNGTKKTGKHLEDLVKEVIELGAGEIILNSIDRDGT
CCCCEEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
MVGYEIEIIKKIQSICKIPITVLGGAGSLDHIKNLIQELGIIGVAAGSLFVYKGVHKAVL
EEEHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEECCCEEEE
INYPNGTDKEALFP
EECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10458921