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The map label for this gene is hisH

Identifier: 116327925

GI number: 116327925

Start: 1427309

End: 1427923

Strand: Direct

Name: hisH

Synonym: LBL_1211

Alternate gene names: 116327925

Gene position: 1427309-1427923 (Clockwise)

Preceding gene: 116327924

Following gene: 116327926

Centisome position: 39.49

GC content: 37.24

Gene sequence:

>615_bases
GTGATTGGAATTTTAGACTACGGAGTCGGAAATTTAAAGGCCTTTGCGAACGTACTTAAAGGTCTGAATTTTCATCATCA
AATCGTTAAAACCGAACAGGAACTAAAAGGTTGTGAAAAAATCATCATGCCGGGTGTGGGTTCGTTCGATAGCGTGATGA
ATAAGTTGATCGAATCGGGCATTCGGGACGTGCTTTCCGATTTAATAATCAACAAAAAAATTCCTATACTAGGCGTTTGT
GTGGGAATGCAAATTTTAGCTTCCTCTAGTGAAGAAGGTAGTAAATCGGGACTTGGTTGGATTCGAGGAAGAGTGAAAAA
GTTTAACTTTGATCGATCCGATTTCTCACTTACTATTCCTCAAATAGGTTGGAACGAAGTTAACTCTACGAAAGAAAATA
CGTTATTAAAAAATTTGGAGAAGAATCCTCGTTTCTATTTCTTACATTCATATTATATAGAATGTGAGGATAAGAAAGAT
GTGATTGCGATCGCGAACTATGGAGGGGATTTTACGTGTGCAGTAAATCGCGAAAATATCTACGGAACTCAGTTTCATCC
CGAAAAAAGTCATCACAATGGAGTCGCCTTAATTCGTAATTTTGCAAGTTTATAA

Upstream 100 bases:

>100_bases
TGGAAAATAAATCCTATAGAGATTACAAATCCACAAGCGGTTTGATCAGTCTTGGAACGAAAATTTTTAGAATGCTTGGG
ATCGAAAAAAGGATCATTCG

Downstream 100 bases:

>100_bases
TTTATGTTAAGACCCAGAATCATACCTGTGTTGTTGTTACAAGAAAACGGATTGGTCAAAACGATCCAATTCGGCGACGA
AAGATATATCGGTGACCCAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIGILDYGVGNLKAFANVLKGLNFHHQIVKTEQELKGCEKIIMPGVGSFDSVMNKLIESGIRDVLSDLIINKKIPILGVC
VGMQILASSSEEGSKSGLGWIRGRVKKFNFDRSDFSLTIPQIGWNEVNSTKENTLLKNLEKNPRFYFLHSYYIECEDKKD
VIAIANYGGDFTCAVNRENIYGTQFHPEKSHHNGVALIRNFASL

Sequences:

>Translated_204_residues
MIGILDYGVGNLKAFANVLKGLNFHHQIVKTEQELKGCEKIIMPGVGSFDSVMNKLIESGIRDVLSDLIINKKIPILGVC
VGMQILASSSEEGSKSGLGWIRGRVKKFNFDRSDFSLTIPQIGWNEVNSTKENTLLKNLEKNPRFYFLHSYYIECEDKKD
VIAIANYGGDFTCAVNRENIYGTQFHPEKSHHNGVALIRNFASL
>Mature_204_residues
MIGILDYGVGNLKAFANVLKGLNFHHQIVKTEQELKGCEKIIMPGVGSFDSVMNKLIESGIRDVLSDLIINKKIPILGVC
VGMQILASSSEEGSKSGLGWIRGRVKKFNFDRSDFSLTIPQIGWNEVNSTKENTLLKNLEKNPRFYFLHSYYIECEDKKD
VIAIANYGGDFTCAVNRENIYGTQFHPEKSHHNGVALIRNFASL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=35.6435643564356, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=34.7417840375587, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_LEPBO (Q9ZGM1)

Other databases:

- EMBL:   AF078135
- ProteinModelPortal:   Q9ZGM1
- SMR:   Q9ZGM1
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22842; Mature: 22842

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 80-80 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGILDYGVGNLKAFANVLKGLNFHHQIVKTEQELKGCEKIIMPGVGSFDSVMNKLIESG
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IRDVLSDLIINKKIPILGVCVGMQILASSSEEGSKSGLGWIRGRVKKFNFDRSDFSLTIP
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECC
QIGWNEVNSTKENTLLKNLEKNPRFYFLHSYYIECEDKKDVIAIANYGGDFTCAVNRENI
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCCEEEEEEECCCE
YGTQFHPEKSHHNGVALIRNFASL
ECCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIGILDYGVGNLKAFANVLKGLNFHHQIVKTEQELKGCEKIIMPGVGSFDSVMNKLIESG
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IRDVLSDLIINKKIPILGVCVGMQILASSSEEGSKSGLGWIRGRVKKFNFDRSDFSLTIP
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECC
QIGWNEVNSTKENTLLKNLEKNPRFYFLHSYYIECEDKKDVIAIANYGGDFTCAVNRENI
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCCEEEEEEECCCE
YGTQFHPEKSHHNGVALIRNFASL
ECCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10458921