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The map label for this gene is nirD [H]

Identifier: 116327452

GI number: 116327452

Start: 747614

End: 747925

Strand: Direct

Name: nirD [H]

Synonym: LBL_0668

Alternate gene names: 116327452

Gene position: 747614-747925 (Clockwise)

Preceding gene: 116327451

Following gene: 116327453

Centisome position: 20.68

GC content: 46.79

Gene sequence:

>312_bases
ATGAGCTTTCGTAAACTCGCAAATCTGTCCGAGGTCGAAAACGGGAAAGTCAAAGTAGTTGAAACCAGATATAATAGAAT
CGGGATCACGAGTTTGGACGGTAACCTTTATGCATTTGAAGACGTCTGTACACATGACGGAGAGGCTATCTCTGAGGGGA
AATTGTGCGGAGACGTAATCACCTGTCCAAGGCATGAAGCGCAGTTTTCCATTAAAACCGGAAAGGCGCTTTGTATGCCC
GCCGTGGAAGATCTCCCCGTATATCCCGTGAGAATCGTAGGTGACTCCATTGAAGTGGATTTGGAGGATTGA

Upstream 100 bases:

>100_bases
TCGTGGAGGGGTTTCTAGGCCAAGTCATCGGTGAAATCGAATCGGATTCTATACAACAAGAGCTTTTCGAGTTGATTGTA
TACAAAGTGGAAGGTCCAAT

Downstream 100 bases:

>100_bases
AATACATGAATTTCTCAGCCGAAAGAATTAGAACCGATTTCCCGATTTTGGGAACAATGATGAACGGAAAACCGCTCGTT
TTTTTGGACAGTGCGGCTAG

Product: nitrite reductase [NAD(P)H], small subunit

Products: cis-cyclohexa-3,5-diene-1,2-diol; NAD+

Alternate protein names: NA

Number of amino acids: Translated: 103; Mature: 102

Protein sequence:

>103_residues
MSFRKLANLSEVENGKVKVVETRYNRIGITSLDGNLYAFEDVCTHDGEAISEGKLCGDVITCPRHEAQFSIKTGKALCMP
AVEDLPVYPVRIVGDSIEVDLED

Sequences:

>Translated_103_residues
MSFRKLANLSEVENGKVKVVETRYNRIGITSLDGNLYAFEDVCTHDGEAISEGKLCGDVITCPRHEAQFSIKTGKALCMP
AVEDLPVYPVRIVGDSIEVDLED
>Mature_102_residues
SFRKLANLSEVENGKVKVVETRYNRIGITSLDGNLYAFEDVCTHDGEAISEGKLCGDVITCPRHEAQFSIKTGKALCMPA
VEDLPVYPVRIVGDSIEVDLED

Specific function: This protein seems to be a 2Fe-2S ferredoxin [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788890, Length=96, Percent_Identity=38.5416666666667, Blast_Score=71, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.12.3

Molecular weight: Translated: 11332; Mature: 11201

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRKLANLSEVENGKVKVVETRYNRIGITSLDGNLYAFEDVCTHDGEAISEGKLCGDVI
CCCHHCCCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEE
TCPRHEAQFSIKTGKALCMPAVEDLPVYPVRIVGDSIEVDLED
ECCCCCCEEEECCCCEEECCCCCCCCCCEEEEECCEEEEEECC
>Mature Secondary Structure 
SFRKLANLSEVENGKVKVVETRYNRIGITSLDGNLYAFEDVCTHDGEAISEGKLCGDVI
CCHHCCCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEE
TCPRHEAQFSIKTGKALCMPAVEDLPVYPVRIVGDSIEVDLED
ECCCCCCEEEECCCCEEECCCCCCCCCCEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: benzene; NADH; H+; O2

Specific reaction: benzene + NADH + H+ + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344526 [H]