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The map label for this gene is ribH

Identifier: 116327423

GI number: 116327423

Start: 705560

End: 706021

Strand: Direct

Name: ribH

Synonym: LBL_0633

Alternate gene names: 116327423

Gene position: 705560-706021 (Clockwise)

Preceding gene: 116327418

Following gene: 116327424

Centisome position: 19.52

GC content: 46.75

Gene sequence:

>462_bases
ATGATCCAAGAACTCAAAGCAGATTTAAACGGAAAAGGACAAAAACACTGTGTGATCGTCTCTCGTTTCAACGAATTTAT
CACGGAAAACCTTTTGAAAGGAGCGCTTGAGTCGTTCCGTATGCACGGAGTTAGAGAAGAAGACGTGACTGTAGTGCGAG
TTCCTGGTGCTTATGAAATGCCGGTCGTAGTTGCCAAAGTCGCCGCTTCTAAAAAATACAATTCCATCGTATGTTTAGGC
GCGGTAATCCGAGGTGCGACCGCTCATTTTGATTTTGTTGCGGGAGAATCCGCAAAGATCGGATCGATCGGAGTTCAACA
TTCCATTCCGGTTGTTTTTGGTGTGTTAACCACGGATACGATTGAACAAGCGATCGAAAGAGCGGGAACCAAAGCCGGGA
ACAAAGGTGCCGAAGCCGCAGCCACGGCGGTGGAAATGGTGAACCTACTTTCTCTTCTTTAA

Upstream 100 bases:

>100_bases
GAAACGATGGGAAAAGTAGAAAAACGATCCTGGGAAGACTTTTTGATTTGCCACAATAGGTAATATGTGATTTCTCAACA
ATAGAATCAATCAGGAATCT

Downstream 100 bases:

>100_bases
ATGTCGGCCAGACGTACTTCTAGAGAAATCGCCGTAATGGCTCTTTACCAATTGGAACTAACGGGACCTCCGCTCAAAGA
GGTTCTCAAGTTCAAATGGT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MIQELKADLNGKGQKHCVIVSRFNEFITENLLKGALESFRMHGVREEDVTVVRVPGAYEMPVVVAKVAASKKYNSIVCLG
AVIRGATAHFDFVAGESAKIGSIGVQHSIPVVFGVLTTDTIEQAIERAGTKAGNKGAEAAATAVEMVNLLSLL

Sequences:

>Translated_153_residues
MIQELKADLNGKGQKHCVIVSRFNEFITENLLKGALESFRMHGVREEDVTVVRVPGAYEMPVVVAKVAASKKYNSIVCLG
AVIRGATAHFDFVAGESAKIGSIGVQHSIPVVFGVLTTDTIEQAIERAGTKAGNKGAEAAATAVEMVNLLSLL
>Mature_153_residues
MIQELKADLNGKGQKHCVIVSRFNEFITENLLKGALESFRMHGVREEDVTVVRVPGAYEMPVVVAKVAASKKYNSIVCLG
AVIRGATAHFDFVAGESAKIGSIGVQHSIPVVFGVLTTDTIEQAIERAGTKAGNKGAEAAATAVEMVNLLSLL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=148, Percent_Identity=50, Blast_Score=155, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324429, Length=140, Percent_Identity=34.2857142857143, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_LEPBJ (Q04Q79)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801699.1
- ProteinModelPortal:   Q04Q79
- SMR:   Q04Q79
- STRING:   Q04Q79
- GeneID:   4412114
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2504
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   Q04Q79
- ProtClustDB:   PRK00061
- BioCyc:   LBOR355277:LBJ_2504-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16238; Mature: 16238

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQELKADLNGKGQKHCVIVSRFNEFITENLLKGALESFRMHGVREEDVTVVRVPGAYEM
CCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
PVVVAKVAASKKYNSIVCLGAVIRGATAHFDFVAGESAKIGSIGVQHSIPVVFGVLTTDT
HHHHHHHHHCCCCCCEEHHHHHHHCCHHCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
IEQAIERAGTKAGNKGAEAAATAVEMVNLLSLL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIQELKADLNGKGQKHCVIVSRFNEFITENLLKGALESFRMHGVREEDVTVVRVPGAYEM
CCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
PVVVAKVAASKKYNSIVCLGAVIRGATAHFDFVAGESAKIGSIGVQHSIPVVFGVLTTDT
HHHHHHHHHCCCCCCEEHHHHHHHCCHHCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
IEQAIERAGTKAGNKGAEAAATAVEMVNLLSLL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA