Click here to switch to the map view.

The map label for this gene is tufB

Identifier: 116327262

GI number: 116327262

Start: 512730

End: 513935

Strand: Direct

Name: tufB

Synonym: LBL_0451

Alternate gene names: 116327262

Gene position: 512730-513935 (Clockwise)

Preceding gene: 116327261

Following gene: 116327263

Centisome position: 14.19

GC content: 46.77

Gene sequence:

>1206_bases
ATGGCTAAAGAAAAGTTTGATAGGTCTAAACCTCACTTAAACGTTGGAACAATTGGTCACGTGGATCACGGTAAAACGAC
CCTGACGGCAGCAATTACTACTACACTTGCAAAAGCGATCGGTGGTAAAAATAAGGCTGTCGCTTATGACCAAATTGATA
ATGCTCCGGAAGAAAAAGCGCGTGGAATCACCATCGCTACTTCTCACCAAGAGTACGAAACCAATAATCGTCACTATGCA
CACGTAGATTGTCCAGGTCACGCCGACTATGTGAAAAACATGATCACAGGCGCGGCTCAGATGGACGCGGCGATCCTCGT
TGTTTCCGCAACCGACGGACCTATGCCACAAACGAAAGAACACATTCTTCTTGCTCGTCAGGTAGGTGTTCCTTACGTGA
TCGTATTCATCAACAAAGCGGACATGCTTGCCGCTGACGAAAGAGCCGAGATGATCGAAATGGTTGAAATGGACGTTCGC
GACCTTCTCAACAAATACAACTTCCCAGGCGATACTACTCCTATCGTTTACGGATCCGCTGTAAAAGCTCTGGAAGGCGA
TGAGTCTGAAATCGGCGCTCCTGCAATCCTCAAACTGATGGAAGCTCTGGATACTTTCGTTCCAAATCCAAAACGCGTTA
CTGATAAGCCTTTCCTTATGCCTGTGGAAGACGTTTTCTCGATCACTGGTCGCGGAACTGTTGCAACAGGAAGAGTGGAA
CAAGGCGTTCTTAAAGTGAACGACGAAGTTGAAATCATCGGTATCCGCCCGACAACTAAAACGGTTGTTACCGGTATTGA
AATGTTCAGAAAACTTTTGGATCAAGCGGAAGCTGGAGACAACATCGGCGCTCTTCTTCGTGGAACTAAAAAAGAAGACA
TCGAAAGAGGACAAGTTCTCGCGAAGCCGGGTTCTATCACTCCTCACAAAAAGTTTGCCGCTGAGGTTTACGTTTTAACT
AAGGATGAAGGCGGACGTCATACTCCGTTCATCAACAACTATCGTCCTCAGTTCTACTTCAGAACGACTGACGTTACCGG
TGTTTGTAACCTTCCTAACGGTGTTGAGATGGTTATGCCTGGTGATAACGTTTCTCTGACAGTTGAATTGATCAGCCCGA
TCGCAATGGATAAAGGTCTCAAGTTCGCAATTCGCGAAGGCGGAAGAACCATTGGATCCGGCGTTGTCGTGGACATCATC
GAGTAA

Upstream 100 bases:

>100_bases
TGTCTCTAGACTCCCTCTTTAACCCTGAACACTATTACGTAATTACATTAGTCGATTCCCGTTAGGGTTCGTTAAAAAGT
AAGGAGATTGAAAAGTCGCT

Downstream 100 bases:

>100_bases
GAGCGCTCTGAAAATGGCCGGACAAAAAATAAGAGTAAAATTGAAAGCGTTCGACCATAGGTTGATTGATCAATCGACCT
ATGAGATCGTAGCAACTGCA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKARGITIATSHQEYETNNRHYA
HVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKEHILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVR
DLLNKYNFPGDTTPIVYGSAVKALEGDESEIGAPAILKLMEALDTFVPNPKRVTDKPFLMPVEDVFSITGRGTVATGRVE
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVLAKPGSITPHKKFAAEVYVLT
KDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMPGDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVVDII
E

Sequences:

>Translated_401_residues
MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKARGITIATSHQEYETNNRHYA
HVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKEHILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVR
DLLNKYNFPGDTTPIVYGSAVKALEGDESEIGAPAILKLMEALDTFVPNPKRVTDKPFLMPVEDVFSITGRGTVATGRVE
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVLAKPGSITPHKKFAAEVYVLT
KDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMPGDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVVDII
E
>Mature_400_residues
AKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKARGITIATSHQEYETNNRHYAH
VDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKEHILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVRD
LLNKYNFPGDTTPIVYGSAVKALEGDESEIGAPAILKLMEALDTFVPNPKRVTDKPFLMPVEDVFSITGRGTVATGRVEQ
GVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVLAKPGSITPHKKFAAEVYVLTK
DEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMPGDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVVDIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=55.3884711779449, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=351, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4503471, Length=352, Percent_Identity=32.9545454545455, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=29.0540540540541, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=29.0540540540541, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI54607086, Length=333, Percent_Identity=30.3303303303303, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=30.1492537313433, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194018520, Length=335, Percent_Identity=30.1492537313433, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=30.1492537313433, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=30.7453416149068, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=25.7462686567164, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=31.3304721030043, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=31.3304721030043, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=33.5766423357664, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=400, Percent_Identity=71.75, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=400, Percent_Identity=71.75, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=367, Percent_Identity=28.8828337874659, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI48994988, Length=279, Percent_Identity=32.258064516129, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789108, Length=440, Percent_Identity=26.3636363636364, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556456, Length=407, Percent_Identity=52.5798525798526, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=392, Percent_Identity=44.3877551020408, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=355, Percent_Identity=32.6760563380282, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=355, Percent_Identity=32.6760563380282, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=345, Percent_Identity=30.7246376811594, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=28.8990825688073, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.8990825688073, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=342, Percent_Identity=28.6549707602339, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=278, Percent_Identity=29.4964028776978, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=176, Percent_Identity=31.8181818181818, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=243, Percent_Identity=28.3950617283951, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17509919, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=401, Percent_Identity=62.0947630922693, Blast_Score=509, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6325337, Length=352, Percent_Identity=33.2386363636364, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=352, Percent_Identity=33.2386363636364, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=26.1363636363636, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=28.8235294117647, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=276, Percent_Identity=25.7246376811594, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=277, Percent_Identity=28.5198555956679, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=149, Percent_Identity=33.5570469798658, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281363316, Length=398, Percent_Identity=55.2763819095477, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=398, Percent_Identity=55.2763819095477, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=47.8149100257069, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=28.0087527352298, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24652838, Length=362, Percent_Identity=31.767955801105, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17137572, Length=362, Percent_Identity=31.767955801105, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=32.8990228013029, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45550900, Length=347, Percent_Identity=29.3948126801153, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=29.5652173913043, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647046, Length=271, Percent_Identity=27.3062730627306, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647048, Length=271, Percent_Identity=27.3062730627306, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_LEPBJ (Q04PT6)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801842.1
- ProteinModelPortal:   Q04PT6
- SMR:   Q04PT6
- STRING:   Q04PT6
- GeneID:   4411200
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2661
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   LBOR355277:LBJ_2661-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43597; Mature: 43465

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKA
CCCCCCCCCCCCEECCEEECCCCCCEEHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHH
RGITIATSHQEYETNNRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKE
CCEEEEECCCHHCCCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCCHH
HILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVRDLLNKYNFPGDTTPIVYGSA
HEEEEHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCH
VKALEGDESEIGAPAILKLMEALDTFVPNPKRVTDKPFLMPVEDVFSITGRGTVATGRVE
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHCCCCCEEEECCHH
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVL
CCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCEEE
AKPGSITPHKKFAAEVYVLTKDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMP
ECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCEEEEC
GDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVVDIIE
CCCCEEEEEECCCHHHCCCCEEEECCCCCEECCCEEEEEEC
>Mature Secondary Structure 
AKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKA
CCCCCCCCCCCEECCEEECCCCCCEEHHHHHHHHHHHHHCCCCCEEEHHHCCCCCHHHH
RGITIATSHQEYETNNRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKE
CCEEEEECCCHHCCCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCCHH
HILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVRDLLNKYNFPGDTTPIVYGSA
HEEEEHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCH
VKALEGDESEIGAPAILKLMEALDTFVPNPKRVTDKPFLMPVEDVFSITGRGTVATGRVE
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHCCCCCEEEECCHH
QGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEDIERGQVL
CCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCEEE
AKPGSITPHKKFAAEVYVLTKDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMP
ECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCEEEEC
GDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVVDIIE
CCCCEEEEEECCCHHHCCCCEEEECCCCCEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA