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The map label for this gene is fusA [H]

Identifier: 116327261

GI number: 116327261

Start: 510811

End: 512694

Strand: Direct

Name: fusA [H]

Synonym: LBL_0450

Alternate gene names: 116327261

Gene position: 510811-512694 (Clockwise)

Preceding gene: 116327260

Following gene: 116327262

Centisome position: 14.13

GC content: 43.58

Gene sequence:

>1884_bases
ATGCAGATTTTAAACGTAGGAATTTTTGCTCACATAGACGCTGGGAAGACGACTCTTTTGGAGAGAATTCTCTTTGAGTC
TGGAAAAATTCGCAAACCTGGTACCATCGAAGATGGGACCACCGAATCCGACTATCTCCAGGAAGAGATTGAACGGGGGA
TCTCCATTCAATCCGCTTTGGCGCGCGTATTTTGGCCGAATGCGAAAAAGCCCAAGGTCTTATTTCAATTTTTGGACAAC
CCGGGGCATTTGGACTTTCAAAGTCAGACGAGTGCCTCTCTCATAGTCGCCGATCTTGGAATCGTTCTTATCGACGCCTT
CGAAGGACTCAAATCTCAAACACTTCAAAATGTGGAATGGCTTCGGAAACGAAAAATTCCGATTTTATTTTTCTTAAACA
AACTGGATCGAAAAGGGATCGATATCACAGATTCCCTTGTGGACTTAGAGGCGGTTTTGGGAAAAGAACCAATTCTTATT
TGGAAGGAGGGAGAGGGTTGTTCGCTTCTTCAAGAGCGGAGTTCCGACCAAGAACTTTTATCCCTTTTGGAATGGGATCC
GAAACTTTCGGAACGATATTTGAAACATCCGGAATCTTTGGCGGAGTTAGCACGGGAAGGATTTGCAGAGGGGTTTTGGA
AAGAGGAGTTTTTCCCGGTTTTGGGAGGTGCCGCTTTGCACGGAGATGGAGTGCGGGAACTTCTCGTCATTCTCGAGCTT
CTTTCTATGTCCTATCGACCGGAACCCCGACCCGGAGAAGAATTGGGAATCGTATTCAAACGAGAACTTCATCCAGATTT
AGGAAAAATTGTCTACATCTTGGCAACGAAAGAGTTTCGACAAAATTCCCCCTTTTACGCAGATACTGGCGAAGGAAGAA
TTGGACCTTGTTATTTTCTTTCTACTCGAGATTTCGAGGAAACTTTTCAAGCAGAACCCCGTGAAATTATTGTTGGGACA
GGTTTGGAATTTCTCAAATCAGGGGATATTCTCTATTCTTCCCCCCAAACTTTTTATCAGTCGAAACTTCCGTCCGTTCG
AAAGCAGTTTCAAATTTTACTCGAACCTGAAGCAGACAAACATCGAGATTCGCTTTGGAGCGCACTACAGACCCTTGTCT
GGTTGGATGAAGGTTTAGAAGCTAAAATCCTTTTAGATACGGGACAAATTCAAATTTCCGGTTTGGGTGAGTTACATCTG
GAGGTTTCTCTTTCTAGACTTCGAGAATTCTTTCCTCACAGATTCAATGTAAGCAGTATTAAAGTTGCAAGGTTTGAGCT
CTGGAAAAAAATGGCCCGACAGGGTGAATTTCAGCATACCGCGTTTGATCAAAAAATCTCAAGCGGACTGGTGCACGCCT
CTTTGGTTAGCTCTAACAGTTTTTCCAGGGATGTGCGGTTTGAAACTAAGATTACTGAAACTCTAGAAGAAGCTATTACA
TCAGCATTTTATGAAGTAGTAGCCAAAGGATCCAAAGGAGAAGAGATTCTCGGTTTGGATCTGATCGTTCATCGTTATGA
TCCTCCGGATACTTTAACGACGGATGTTTCTTCCCTTGTAAAAGTTGCCGTTATTAAAGGTTTAAAGGACATAATTCCGA
AATACACGGAGTTAGTCGGTCCCGTTTCTACTGTGGAGATTTTGATACCGGATGTTTCATTAGGAGACGTTTTGGGTTCT
CTCTCGAAGCGAAACGCTAAAATTCAGGAAGTGGTTCCTCTCGGTGACGGCAAGTCGCTTGTTCATGCAAAGGCTTCTAC
GGAAAACTTGCTTGGCTTTGCAGGCGTTCTTAGAAATATGACACAGGGAAGGGGTGTTCTGTCTCTAGACTCCCTCTTTA
ACCCTGAACACTATTACGTAATTACATTAGTCGATTCCCGTTAG

Upstream 100 bases:

>100_bases
GAAAAACTTTACGATTCCCCGTTGGTTTTGTTTGAACGTTGGTTCCTTGGAAAAAAGTAGGAACTCCTAAAAGGAGGAGA
GGAGGATTCAGAAAATCGCC

Downstream 100 bases:

>100_bases
GGTTCGTTAAAAAGTAAGGAGATTGAAAAGTCGCTATGGCTAAAGAAAAGTTTGATAGGTCTAAACCTCACTTAAACGTT
GGAACAATTGGTCACGTGGA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MQILNVGIFAHIDAGKTTLLERILFESGKIRKPGTIEDGTTESDYLQEEIERGISIQSALARVFWPNAKKPKVLFQFLDN
PGHLDFQSQTSASLIVADLGIVLIDAFEGLKSQTLQNVEWLRKRKIPILFFLNKLDRKGIDITDSLVDLEAVLGKEPILI
WKEGEGCSLLQERSSDQELLSLLEWDPKLSERYLKHPESLAELAREGFAEGFWKEEFFPVLGGAALHGDGVRELLVILEL
LSMSYRPEPRPGEELGIVFKRELHPDLGKIVYILATKEFRQNSPFYADTGEGRIGPCYFLSTRDFEETFQAEPREIIVGT
GLEFLKSGDILYSSPQTFYQSKLPSVRKQFQILLEPEADKHRDSLWSALQTLVWLDEGLEAKILLDTGQIQISGLGELHL
EVSLSRLREFFPHRFNVSSIKVARFELWKKMARQGEFQHTAFDQKISSGLVHASLVSSNSFSRDVRFETKITETLEEAIT
SAFYEVVAKGSKGEEILGLDLIVHRYDPPDTLTTDVSSLVKVAVIKGLKDIIPKYTELVGPVSTVEILIPDVSLGDVLGS
LSKRNAKIQEVVPLGDGKSLVHAKASTENLLGFAGVLRNMTQGRGVLSLDSLFNPEHYYVITLVDSR

Sequences:

>Translated_627_residues
MQILNVGIFAHIDAGKTTLLERILFESGKIRKPGTIEDGTTESDYLQEEIERGISIQSALARVFWPNAKKPKVLFQFLDN
PGHLDFQSQTSASLIVADLGIVLIDAFEGLKSQTLQNVEWLRKRKIPILFFLNKLDRKGIDITDSLVDLEAVLGKEPILI
WKEGEGCSLLQERSSDQELLSLLEWDPKLSERYLKHPESLAELAREGFAEGFWKEEFFPVLGGAALHGDGVRELLVILEL
LSMSYRPEPRPGEELGIVFKRELHPDLGKIVYILATKEFRQNSPFYADTGEGRIGPCYFLSTRDFEETFQAEPREIIVGT
GLEFLKSGDILYSSPQTFYQSKLPSVRKQFQILLEPEADKHRDSLWSALQTLVWLDEGLEAKILLDTGQIQISGLGELHL
EVSLSRLREFFPHRFNVSSIKVARFELWKKMARQGEFQHTAFDQKISSGLVHASLVSSNSFSRDVRFETKITETLEEAIT
SAFYEVVAKGSKGEEILGLDLIVHRYDPPDTLTTDVSSLVKVAVIKGLKDIIPKYTELVGPVSTVEILIPDVSLGDVLGS
LSKRNAKIQEVVPLGDGKSLVHAKASTENLLGFAGVLRNMTQGRGVLSLDSLFNPEHYYVITLVDSR
>Mature_627_residues
MQILNVGIFAHIDAGKTTLLERILFESGKIRKPGTIEDGTTESDYLQEEIERGISIQSALARVFWPNAKKPKVLFQFLDN
PGHLDFQSQTSASLIVADLGIVLIDAFEGLKSQTLQNVEWLRKRKIPILFFLNKLDRKGIDITDSLVDLEAVLGKEPILI
WKEGEGCSLLQERSSDQELLSLLEWDPKLSERYLKHPESLAELAREGFAEGFWKEEFFPVLGGAALHGDGVRELLVILEL
LSMSYRPEPRPGEELGIVFKRELHPDLGKIVYILATKEFRQNSPFYADTGEGRIGPCYFLSTRDFEETFQAEPREIIVGT
GLEFLKSGDILYSSPQTFYQSKLPSVRKQFQILLEPEADKHRDSLWSALQTLVWLDEGLEAKILLDTGQIQISGLGELHL
EVSLSRLREFFPHRFNVSSIKVARFELWKKMARQGEFQHTAFDQKISSGLVHASLVSSNSFSRDVRFETKITETLEEAIT
SAFYEVVAKGSKGEEILGLDLIVHRYDPPDTLTTDVSSLVKVAVIKGLKDIIPKYTELVGPVSTVEILIPDVSLGDVLGS
LSKRNAKIQEVVPLGDGKSLVHAKASTENLLGFAGVLRNMTQGRGVLSLDSLFNPEHYYVITLVDSR

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=696, Percent_Identity=23.2758620689655, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI19923640, Length=293, Percent_Identity=31.3993174061433, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI25306287, Length=293, Percent_Identity=31.3993174061433, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI25306283, Length=293, Percent_Identity=31.3993174061433, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI157426893, Length=140, Percent_Identity=34.2857142857143, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272892, Length=136, Percent_Identity=30.1470588235294, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI217272894, Length=136, Percent_Identity=30.1470588235294, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI94966754, Length=140, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1789738, Length=697, Percent_Identity=24.8206599713056, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790835, Length=169, Percent_Identity=35.5029585798817, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI48994988, Length=151, Percent_Identity=27.8145695364238, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=697, Percent_Identity=24.2467718794835, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=488, Percent_Identity=25, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=141, Percent_Identity=36.1702127659575, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=144, Percent_Identity=28.4722222222222, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=144, Percent_Identity=28.4722222222222, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=696, Percent_Identity=23.132183908046, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322359, Length=715, Percent_Identity=22.9370629370629, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322675, Length=147, Percent_Identity=31.2925170068027, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=139, Percent_Identity=31.6546762589928, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=695, Percent_Identity=24.6043165467626, Blast_Score=147, Evalue=3e-35,
Organism=Drosophila melanogaster, GI221458488, Length=711, Percent_Identity=22.9254571026723, Blast_Score=142, Evalue=9e-34,
Organism=Drosophila melanogaster, GI78706572, Length=177, Percent_Identity=34.4632768361582, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=29.1390728476821, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=29.1390728476821, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=29.1390728476821, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574573, Length=162, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 70327; Mature: 70327

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILNVGIFAHIDAGKTTLLERILFESGKIRKPGTIEDGTTESDYLQEEIERGISIQSAL
CEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ARVFWPNAKKPKVLFQFLDNPGHLDFQSQTSASLIVADLGIVLIDAFEGLKSQTLQNVEW
HHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKRKIPILFFLNKLDRKGIDITDSLVDLEAVLGKEPILIWKEGEGCSLLQERSSDQELL
HHHCCCCCEEEEHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCHHHHH
SLLEWDPKLSERYLKHPESLAELAREGFAEGFWKEEFFPVLGGAALHGDGVRELLVILEL
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCHHHHHHHHHH
LSMSYRPEPRPGEELGIVFKRELHPDLGKIVYILATKEFRQNSPFYADTGEGRIGPCYFL
HHCCCCCCCCCCCHHCEEEEHHCCCCHHHEEEEEEEHHHHCCCCCEEECCCCCCCCEEEE
STRDFEETFQAEPREIIVGTGLEFLKSGDILYSSPQTFYQSKLPSVRKQFQILLEPEADK
CCCCHHHHHCCCCCEEEEECCHHHHHCCCEEECCCHHHHHHHCHHHHHHHEEEECCCCCH
HRDSLWSALQTLVWLDEGLEAKILLDTGQIQISGLGELHLEVSLSRLREFFPHRFNVSSI
HHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCEEEHHHHHHHHHHHCCCCCCCCHH
KVARFELWKKMARQGEFQHTAFDQKISSGLVHASLVSSNSFSRDVRFETKITETLEEAIT
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEHHCCCCCCCCEEEHHHHHHHHHHHHH
SAFYEVVAKGSKGEEILGLDLIVHRYDPPDTLTTDVSSLVKVAVIKGLKDIIPKYTELVG
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PVSTVEILIPDVSLGDVLGSLSKRNAKIQEVVPLGDGKSLVHAKASTENLLGFAGVLRNM
CCCEEEEEECCCCHHHHHHHHHHCCCCHHEEEECCCCCCEEEECCCHHHHHHHHHHHHHH
TQGRGVLSLDSLFNPEHYYVITLVDSR
HCCCCEEEHHHHCCCCEEEEEEEECCC
>Mature Secondary Structure
MQILNVGIFAHIDAGKTTLLERILFESGKIRKPGTIEDGTTESDYLQEEIERGISIQSAL
CEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ARVFWPNAKKPKVLFQFLDNPGHLDFQSQTSASLIVADLGIVLIDAFEGLKSQTLQNVEW
HHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKRKIPILFFLNKLDRKGIDITDSLVDLEAVLGKEPILIWKEGEGCSLLQERSSDQELL
HHHCCCCCEEEEHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCHHHHH
SLLEWDPKLSERYLKHPESLAELAREGFAEGFWKEEFFPVLGGAALHGDGVRELLVILEL
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCHHHHHHHHHH
LSMSYRPEPRPGEELGIVFKRELHPDLGKIVYILATKEFRQNSPFYADTGEGRIGPCYFL
HHCCCCCCCCCCCHHCEEEEHHCCCCHHHEEEEEEEHHHHCCCCCEEECCCCCCCCEEEE
STRDFEETFQAEPREIIVGTGLEFLKSGDILYSSPQTFYQSKLPSVRKQFQILLEPEADK
CCCCHHHHHCCCCCEEEEECCHHHHHCCCEEECCCHHHHHHHCHHHHHHHEEEECCCCCH
HRDSLWSALQTLVWLDEGLEAKILLDTGQIQISGLGELHLEVSLSRLREFFPHRFNVSSI
HHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCEEEHHHHHHHHHHHCCCCCCCCHH
KVARFELWKKMARQGEFQHTAFDQKISSGLVHASLVSSNSFSRDVRFETKITETLEEAIT
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEHHCCCCCCCCEEEHHHHHHHHHHHHH
SAFYEVVAKGSKGEEILGLDLIVHRYDPPDTLTTDVSSLVKVAVIKGLKDIIPKYTELVG
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PVSTVEILIPDVSLGDVLGSLSKRNAKIQEVVPLGDGKSLVHAKASTENLLGFAGVLRNM
CCCEEEEEECCCCHHHHHHHHHHCCCCHHEEEECCCCCCEEEECCCHHHHHHHHHHHHHH
TQGRGVLSLDSLFNPEHYYVITLVDSR
HCCCCEEEHHHHCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA