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The map label for this gene is phaZ

Identifier: 114800245

GI number: 114800245

Start: 167642

End: 168940

Strand: Direct

Name: phaZ

Synonym: HNE_0180

Alternate gene names: NA

Gene position: 167642-168940 (Clockwise)

Preceding gene: 114798757

Following gene: 114798689

Centisome position: 4.52

GC content: 59.58

Gene sequence:

>1299_bases
ATGCTCTATTCCCTTGTCGAACTGAACCGGGTTGCGATGGCGCCAATGCGGCTTGCCGCGCGCGCCGGGCTCGCCGCCCT
GAATTCGCCGTTTAACCCGATGGGGCAGACCAGCTACGGCAAGTCGCTCGCGGCGATGGCGGACGTGTTCGAGAGCGCGA
CCCGCTATTACGGCAAACCCGAATGGCGGATCGGTTCGGTCCGTATAAACAGCATTGATGTGCCGGTAGAACCGCAGATC
TCCTGGCGGTCACCCTGGTGTAGTCTGGTTCATTTCAAAAAAGACGCGGGTCGCCTTGCCGAAGCGCGCCGCAAGGGCGC
AGCGCCGCTCCCGCGCATGCTGATTGTGGCGCCATTGTCGGGCCATTATGCAACGCTTCTGCGCGGAACCGTCGAGGGAT
TTCTCGAAACACACGATGTCTACATCACTGACTGGACGGACGCGCGTATGGCGCCGGTCTGGCTCGGCCGGTTCGATCTT
GACGATTACATTGATCATATTCGCAAGGTGCTCACACATCTGGGCGGCGGTGTGCATGTGCTGGCCGTTTGCCAGCCCGG
TCCGCCTGTGTTGGCGGCGATCGCGATGATGGCAGAAGATGATGATCCGGCGCGTCCGGCGACCATGACATTCATGGGCT
CTCCGATTGATGCGCGCCGCTCGCCGACCATTCCAAACAAGCTGGCGGAAGAGCGCCCGTTTGAATGGTTCGAACAGAAC
ATGATTTACACGGTGCCTGCGCCCTATCCGGGCATGCTGCGCCGCGTATACCCCGGATTTGTGCAGCTTTCCTCGTTCAT
GAACATGAACTGGGACCGGCATGTCGATGCCCACTGGAAGTTCTTCAACCACCTGGTTGGCGGCGACGGTGACAATGCCG
GAAGACACCGCGAGTTCTATGATGAATATCTTTCCGTCCTTGATCTGACGGAGGAGTTTTATCTTCAGACCATTCAGCGG
GTCTTCCAGGAGCACCATATCCCGCGGGGCATCTACAGCTACAGGGGCGCGCGGCTGGTGAAGCCGGAGGCAATTCGCGA
TGTAGCCCTGATGACCGTTGAGGGGGAAAAAGACGACATCTCCGGTATCGGCCAGACGCAAGCCGCGCACGATCTCTGTA
CGAATATTCCAGAAGATATGCGAACGGATTATATTCAGCCGGGCGTCGGCCACTACGGCGTCTTCAACGGCTCACGGTTC
GAGACGGAAATTGCGCCGCGTGTGGCACAGTTCACCCGGCGCTTTACCGATCGTGAGAAGGAAGAAGCGGCTCACAGCGT
GGCCGCGGCCCGCCGCTAA

Upstream 100 bases:

>100_bases
CCTCAAGCTTCCCTGTGGCGTGCGCCCCGCTGGCGAAAACATTTGCCGGACCGATTATAGAGGGGTACGCTGCACTGCAA
CATAAAGGGTCTGAAAGACG

Downstream 100 bases:

>100_bases
GCTGGTCTAGCCGCAGATCGTCGCCTCGAAGTCCAGCAACTCAATCTCTGGCGTGAGGTGGCCCTCCTTATGCGGCTCTT
CGCATGCCAGTGCGCCGGAG

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Polyhydroxybutanoate Depolymerase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxybutyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLYSLVELNRVAMAPMRLAARAGLAALNSPFNPMGQTSYGKSLAAMADVFESATRYYGKPEWRIGSVRINSIDVPVEPQI
SWRSPWCSLVHFKKDAGRLAEARRKGAAPLPRMLIVAPLSGHYATLLRGTVEGFLETHDVYITDWTDARMAPVWLGRFDL
DDYIDHIRKVLTHLGGGVHVLAVCQPGPPVLAAIAMMAEDDDPARPATMTFMGSPIDARRSPTIPNKLAEERPFEWFEQN
MIYTVPAPYPGMLRRVYPGFVQLSSFMNMNWDRHVDAHWKFFNHLVGGDGDNAGRHREFYDEYLSVLDLTEEFYLQTIQR
VFQEHHIPRGIYSYRGARLVKPEAIRDVALMTVEGEKDDISGIGQTQAAHDLCTNIPEDMRTDYIQPGVGHYGVFNGSRF
ETEIAPRVAQFTRRFTDREKEEAAHSVAAARR

Sequences:

>Translated_432_residues
MLYSLVELNRVAMAPMRLAARAGLAALNSPFNPMGQTSYGKSLAAMADVFESATRYYGKPEWRIGSVRINSIDVPVEPQI
SWRSPWCSLVHFKKDAGRLAEARRKGAAPLPRMLIVAPLSGHYATLLRGTVEGFLETHDVYITDWTDARMAPVWLGRFDL
DDYIDHIRKVLTHLGGGVHVLAVCQPGPPVLAAIAMMAEDDDPARPATMTFMGSPIDARRSPTIPNKLAEERPFEWFEQN
MIYTVPAPYPGMLRRVYPGFVQLSSFMNMNWDRHVDAHWKFFNHLVGGDGDNAGRHREFYDEYLSVLDLTEEFYLQTIQR
VFQEHHIPRGIYSYRGARLVKPEAIRDVALMTVEGEKDDISGIGQTQAAHDLCTNIPEDMRTDYIQPGVGHYGVFNGSRF
ETEIAPRVAQFTRRFTDREKEEAAHSVAAARR
>Mature_432_residues
MLYSLVELNRVAMAPMRLAARAGLAALNSPFNPMGQTSYGKSLAAMADVFESATRYYGKPEWRIGSVRINSIDVPVEPQI
SWRSPWCSLVHFKKDAGRLAEARRKGAAPLPRMLIVAPLSGHYATLLRGTVEGFLETHDVYITDWTDARMAPVWLGRFDL
DDYIDHIRKVLTHLGGGVHVLAVCQPGPPVLAAIAMMAEDDDPARPATMTFMGSPIDARRSPTIPNKLAEERPFEWFEQN
MIYTVPAPYPGMLRRVYPGFVQLSSFMNMNWDRHVDAHWKFFNHLVGGDGDNAGRHREFYDEYLSVLDLTEEFYLQTIQR
VFQEHHIPRGIYSYRGARLVKPEAIRDVALMTVEGEKDDISGIGQTQAAHDLCTNIPEDMRTDYIQPGVGHYGVFNGSRF
ETEIAPRVAQFTRRFTDREKEEAAHSVAAARR

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 48784; Mature: 48784

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSLVELNRVAMAPMRLAARAGLAALNSPFNPMGQTSYGKSLAAMADVFESATRYYGKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EWRIGSVRINSIDVPVEPQISWRSPWCSLVHFKKDAGRLAEARRKGAAPLPRMLIVAPLS
CEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
GHYATLLRGTVEGFLETHDVYITDWTDARMAPVWLGRFDLDDYIDHIRKVLTHLGGGVHV
CHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEE
LAVCQPGPPVLAAIAMMAEDDDPARPATMTFMGSPIDARRSPTIPNKLAEERPFEWFEQN
EEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHCC
MIYTVPAPYPGMLRRVYPGFVQLSSFMNMNWDRHVDAHWKFFNHLVGGDGDNAGRHREFY
EEEECCCCCHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
DEYLSVLDLTEEFYLQTIQRVFQEHHIPRGIYSYRGARLVKPEAIRDVALMTVEGEKDDI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECHHHHCCEEEEEECCCCCCC
SGIGQTQAAHDLCTNIPEDMRTDYIQPGVGHYGVFNGSRFETEIAPRVAQFTRRFTDREK
CCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
EEAAHSVAAARR
HHHHHHHHHHCC
>Mature Secondary Structure
MLYSLVELNRVAMAPMRLAARAGLAALNSPFNPMGQTSYGKSLAAMADVFESATRYYGKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EWRIGSVRINSIDVPVEPQISWRSPWCSLVHFKKDAGRLAEARRKGAAPLPRMLIVAPLS
CEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
GHYATLLRGTVEGFLETHDVYITDWTDARMAPVWLGRFDLDDYIDHIRKVLTHLGGGVHV
CHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEE
LAVCQPGPPVLAAIAMMAEDDDPARPATMTFMGSPIDARRSPTIPNKLAEERPFEWFEQN
EEEECCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHCC
MIYTVPAPYPGMLRRVYPGFVQLSSFMNMNWDRHVDAHWKFFNHLVGGDGDNAGRHREFY
EEEECCCCCHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
DEYLSVLDLTEEFYLQTIQRVFQEHHIPRGIYSYRGARLVKPEAIRDVALMTVEGEKDDI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECHHHHCCEEEEEECCCCCCC
SGIGQTQAAHDLCTNIPEDMRTDYIQPGVGHYGVFNGSRFETEIAPRVAQFTRRFTDREK
CCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHH
EEAAHSVAAARR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA