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The map label for this gene is pepA [H]

Identifier: 114800113

GI number: 114800113

Start: 3175555

End: 3176925

Strand: Reverse

Name: pepA [H]

Synonym: HNE_3007

Alternate gene names: 114800113

Gene position: 3176925-3175555 (Counterclockwise)

Preceding gene: 114798130

Following gene: 114799126

Centisome position: 85.75

GC content: 66.67

Gene sequence:

>1371_bases
ATGCACAAGTCCTTCACCTCCGATGCACCCCAAACCGTTCGGGTATGGCTCTATACGGGCACCCTGTTCGGCGCGCTGGA
GAACGATCCGTTCCCCAATGCGCGGGCGATTGCGGAGGCGCAGGGCTTCACCGGCGGAGCAGGGCAGAGCGTGATCGTGC
CCGGCCCGGATGGCCGCACAAGCGACATTCTCTACGGTCTAGGCGCTGGCCGGGATCAGCTCGCGCTCGCCGCCCTGTCG
GCAAAGCTTCCTGCGGGTGACTATGAAATCGCCGCGGACGGCGGCTACCCCTTCGCCAGCATCGCCGCGGGCTGGGCAGA
TGGGGCCTACCGGTTCGACGGGTACCGGACTGAAAAGGCCGCCCCGCCGCGCCTTCTTATCCCGGCGTCGGAAGATGCCC
CGCGCCTGTCGCGCGAGGCAGACGCAGTTAGCGCCCTGCGCGATCTAGTGAACACGCCGGCGGCCGATATGGGCCCCGAA
CAGATCCATGCGCGGATCAGCGCGCTGGGCGAGCGTCATGGCGCGCGCGTCGCCGCCGTCGTGGGCGACGCGCTGATCGA
TGAGAATTATCCCATGGTCCATGCGGTCGGGCGCGCGGCGGTCAAGGCGCCCCGTTTCGTCATGCTGGAATGGGGCAAGC
CCGGCGCACCCAAAGTCTCGCTCTGCGGCAAGGGCATCACCTTTGATACCGGCGGGCTCAACATCAAGACCGGCGACGGC
ATGCGCATCATGAAGAAGGATATGGGCGGGGCCGCCCATGCCATCGCGCTGGCTGAGCTCGTCATGGAAGCGCGCCTGCC
GGTGCATCTGCGCCTCTTTGTATCCGCCGCCGAAAACGCCATTGCGGGCGACGCGTTCCGCCCCGGTGACATCCTGCAAA
GCCGCAAGGGCCTCACCGTCGAGATCGACAATACAGATGCTGAAGGCCGCCTCGTCCTGGCCGACGCGCTCACCCGCGCC
AGCGAAGACCAGCCCGAGCTGCTGATCGACTTTGCGACCCTCACCGGCGCTGCCCGCGTGGCTCTCGGCCCGGACCTCGC
CCCGCTCTATACCGATGACGAGCAGCTCGCTGCCGACATCCTCGCCGCCTCCGAAGAGACCGGCGATCCTGTCTGGCGCA
TGCCGCTCTGGGACCCCTATCTCGCCTATATGCAAAGCCCCGTCGCTGACCTTGTAAATTCGGGCGGGGCAGGGATGGCC
GGCTCGATCACGGCCGCCATCTTCCTCAAACAGTTCGTCTCGGCCAAAAGCTGGGCGCATTTCGACATCTGGGCCTGGCG
TAAAGCCAAATATGGCCGCCCCGATGGCGGCGCCGCCTGCGGTCTGCGCGCGGTCTGGGCGATGCTGGAAAAACGCTATC
CGGCGGTCTGA

Upstream 100 bases:

>100_bases
TCCCTGAGGGTCGAAGCAGACTTTGCCCCCGGCCGGGTCAAAGTTCGGTTAACGCCGGAGCCTTCGCCATTAACCACCGA
CACGCCCGGAAAGGCTCAGT

Downstream 100 bases:

>100_bases
AGAAAAGTCCGTATACAGGTATTTCTGGCGGTGCGGTTATCACGTCGCGGCGTAATATAACCCGGATTTAGATCGCCTTT
TTGGGGTTGACCCCGAATCT

Product: cytosol aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MHKSFTSDAPQTVRVWLYTGTLFGALENDPFPNARAIAEAQGFTGGAGQSVIVPGPDGRTSDILYGLGAGRDQLALAALS
AKLPAGDYEIAADGGYPFASIAAGWADGAYRFDGYRTEKAAPPRLLIPASEDAPRLSREADAVSALRDLVNTPAADMGPE
QIHARISALGERHGARVAAVVGDALIDENYPMVHAVGRAAVKAPRFVMLEWGKPGAPKVSLCGKGITFDTGGLNIKTGDG
MRIMKKDMGGAAHAIALAELVMEARLPVHLRLFVSAAENAIAGDAFRPGDILQSRKGLTVEIDNTDAEGRLVLADALTRA
SEDQPELLIDFATLTGAARVALGPDLAPLYTDDEQLAADILAASEETGDPVWRMPLWDPYLAYMQSPVADLVNSGGAGMA
GSITAAIFLKQFVSAKSWAHFDIWAWRKAKYGRPDGGAACGLRAVWAMLEKRYPAV

Sequences:

>Translated_456_residues
MHKSFTSDAPQTVRVWLYTGTLFGALENDPFPNARAIAEAQGFTGGAGQSVIVPGPDGRTSDILYGLGAGRDQLALAALS
AKLPAGDYEIAADGGYPFASIAAGWADGAYRFDGYRTEKAAPPRLLIPASEDAPRLSREADAVSALRDLVNTPAADMGPE
QIHARISALGERHGARVAAVVGDALIDENYPMVHAVGRAAVKAPRFVMLEWGKPGAPKVSLCGKGITFDTGGLNIKTGDG
MRIMKKDMGGAAHAIALAELVMEARLPVHLRLFVSAAENAIAGDAFRPGDILQSRKGLTVEIDNTDAEGRLVLADALTRA
SEDQPELLIDFATLTGAARVALGPDLAPLYTDDEQLAADILAASEETGDPVWRMPLWDPYLAYMQSPVADLVNSGGAGMA
GSITAAIFLKQFVSAKSWAHFDIWAWRKAKYGRPDGGAACGLRAVWAMLEKRYPAV
>Mature_456_residues
MHKSFTSDAPQTVRVWLYTGTLFGALENDPFPNARAIAEAQGFTGGAGQSVIVPGPDGRTSDILYGLGAGRDQLALAALS
AKLPAGDYEIAADGGYPFASIAAGWADGAYRFDGYRTEKAAPPRLLIPASEDAPRLSREADAVSALRDLVNTPAADMGPE
QIHARISALGERHGARVAAVVGDALIDENYPMVHAVGRAAVKAPRFVMLEWGKPGAPKVSLCGKGITFDTGGLNIKTGDG
MRIMKKDMGGAAHAIALAELVMEARLPVHLRLFVSAAENAIAGDAFRPGDILQSRKGLTVEIDNTDAEGRLVLADALTRA
SEDQPELLIDFATLTGAARVALGPDLAPLYTDDEQLAADILAASEETGDPVWRMPLWDPYLAYMQSPVADLVNSGGAGMA
GSITAAIFLKQFVSAKSWAHFDIWAWRKAKYGRPDGGAACGLRAVWAMLEKRYPAV

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=370, Percent_Identity=33.7837837837838, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI47155554, Length=298, Percent_Identity=32.8859060402685, Blast_Score=132, Evalue=9e-31,
Organism=Escherichia coli, GI87082123, Length=362, Percent_Identity=38.6740331491713, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1790710, Length=349, Percent_Identity=36.3896848137536, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17556903, Length=306, Percent_Identity=34.9673202614379, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17565172, Length=236, Percent_Identity=30.5084745762712, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355725, Length=288, Percent_Identity=34.0277777777778, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24661038, Length=288, Percent_Identity=34.0277777777778, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221379063, Length=297, Percent_Identity=32.996632996633, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI221379062, Length=297, Percent_Identity=32.996632996633, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21357381, Length=297, Percent_Identity=32.996632996633, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24662227, Length=346, Percent_Identity=26.5895953757225, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129969, Length=346, Percent_Identity=26.878612716763, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI19922386, Length=321, Percent_Identity=28.3489096573209, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161077148, Length=349, Percent_Identity=28.6532951289398, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130057, Length=349, Percent_Identity=28.6532951289398, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355645, Length=325, Percent_Identity=25.5384615384615, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24662223, Length=325, Percent_Identity=25.5384615384615, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129963, Length=323, Percent_Identity=28.4829721362229, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646701, Length=201, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24646703, Length=201, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21358201, Length=201, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283 [H]

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 48194; Mature: 48194

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKSFTSDAPQTVRVWLYTGTLFGALENDPFPNARAIAEAQGFTGGAGQSVIVPGPDGRT
CCCCCCCCCCCEEEEEEEECEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCC
SDILYGLGAGRDQLALAALSAKLPAGDYEIAADGGYPFASIAAGWADGAYRFDGYRTEKA
CCEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCEEECCEECCCC
APPRLLIPASEDAPRLSREADAVSALRDLVNTPAADMGPEQIHARISALGERHGARVAAV
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
VGDALIDENYPMVHAVGRAAVKAPRFVMLEWGKPGAPKVSLCGKGITFDTGGLNIKTGDG
HHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCEECCCCEEEECCCC
MRIMKKDMGGAAHAIALAELVMEARLPVHLRLFVSAAENAIAGDAFRPGDILQSRKGLTV
CCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCCCCHHHHHCCCCCEE
EIDNTDAEGRLVLADALTRASEDQPELLIDFATLTGAARVALGPDLAPLYTDDEQLAADI
EECCCCCCCEEEEEHHHHHCCCCCCCEEEEEHHHCCCEEEEECCCCCCCCCCHHHHHHHH
LAASEETGDPVWRMPLWDPYLAYMQSPVADLVNSGGAGMAGSITAAIFLKQFVSAKSWAH
HHHCCCCCCCEEECCCCCHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCE
FDIWAWRKAKYGRPDGGAACGLRAVWAMLEKRYPAV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHKSFTSDAPQTVRVWLYTGTLFGALENDPFPNARAIAEAQGFTGGAGQSVIVPGPDGRT
CCCCCCCCCCCEEEEEEEECEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCC
SDILYGLGAGRDQLALAALSAKLPAGDYEIAADGGYPFASIAAGWADGAYRFDGYRTEKA
CCEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCEEECCEECCCC
APPRLLIPASEDAPRLSREADAVSALRDLVNTPAADMGPEQIHARISALGERHGARVAAV
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
VGDALIDENYPMVHAVGRAAVKAPRFVMLEWGKPGAPKVSLCGKGITFDTGGLNIKTGDG
HHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCEECCCCEEEECCCC
MRIMKKDMGGAAHAIALAELVMEARLPVHLRLFVSAAENAIAGDAFRPGDILQSRKGLTV
CCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCCCCHHHHHCCCCCEE
EIDNTDAEGRLVLADALTRASEDQPELLIDFATLTGAARVALGPDLAPLYTDDEQLAADI
EECCCCCCCEEEEEHHHHHCCCCCCCEEEEEHHHCCCEEEEECCCCCCCCCCHHHHHHHH
LAASEETGDPVWRMPLWDPYLAYMQSPVADLVNSGGAGMAGSITAAIFLKQFVSAKSWAH
HHHCCCCCCCEEECCCCCHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCE
FDIWAWRKAKYGRPDGGAACGLRAVWAMLEKRYPAV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]