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The map label for this gene is lipB

Identifier: 114800027

GI number: 114800027

Start: 1071345

End: 1072049

Strand: Reverse

Name: lipB

Synonym: HNE_1062

Alternate gene names: 114800027

Gene position: 1072049-1071345 (Counterclockwise)

Preceding gene: 114797853

Following gene: 114799828

Centisome position: 28.94

GC content: 68.79

Gene sequence:

>705_bases
ATGCAGAATTTTCCCCCCGTCGAATGGGCCGTGTCAGATGCCCCCGTGCCCTATGAGGCGGCGCTCGCCGTCATGGAGGC
CCGCGCCCGCGCGATTCGCGAGGACGGCGCCCCGGAGCTGGTGTGGTTTCTGGAACATCCCCCCCTCTACACCGCCGGCA
CCTCCGCCCGCATCGAAGACCTGAAAGACCCCGCCCGCTTCCCCGTCTTTGAAGCCGGGCGCGGCGGCCAGTACACCTAT
CACGGCCCCGGCCAGCGGGTGGCCTATGTCATGCTCGATGTCGCCGCCCGCGGCCGCGATGTGCGCGCCTTCGTGGCCCA
TCTGGAAGCCTGGATCATCCAGGCGCTGGCCGCGTTCAATGTCGAAGGCGGCATGCGCGACGGCCGTGTCGGGGTCTGGG
TCGACCGCACAGAGCCCGGCGGCCCGCCCCGCGAAGACAAGATCGCCGCCATCGGCGTGCGCCTGAAGCGCTGGGTGTCC
TTCCACGGCATCTCGCTGAACGTCGAGCCGGAGCTCGACCATTTCACCGGCATCACCCCCTGCGGCATCGCAGACCCCCG
CTACGGCGTCACCAGCCTGGCCGACCTCGGCCGTATAATCTCGATGGAAGAAGCCGACATCGCCCTCAAGGCGGCCTTCC
AGCAGGTCTTCGCCAGCCCCCTGGTCCGCGTTGACCCACCCGTGACAGGATCTGTCCCGGCCTGA

Upstream 100 bases:

>100_bases
TTCCGCCACGACCGCACACTGGTATGTCGAGACAGACGGGCCGTATAAGGCGGTCATTCGCCTTTGTGAAGCCCTGAAAA
CACCCTATATCGCACCGGCA

Downstream 100 bases:

>100_bases
CCGGATCTGTCCCGGAAGTGTCCGGACTTGTCCTGAACTGTCCGGATCTGACCTCACCGCGCAACGCAACAGGCCCCCTC
CCCATGACTATGGGGGCTGT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MQNFPPVEWAVSDAPVPYEAALAVMEARARAIREDGAPELVWFLEHPPLYTAGTSARIEDLKDPARFPVFEAGRGGQYTY
HGPGQRVAYVMLDVAARGRDVRAFVAHLEAWIIQALAAFNVEGGMRDGRVGVWVDRTEPGGPPREDKIAAIGVRLKRWVS
FHGISLNVEPELDHFTGITPCGIADPRYGVTSLADLGRIISMEEADIALKAAFQQVFASPLVRVDPPVTGSVPA

Sequences:

>Translated_234_residues
MQNFPPVEWAVSDAPVPYEAALAVMEARARAIREDGAPELVWFLEHPPLYTAGTSARIEDLKDPARFPVFEAGRGGQYTY
HGPGQRVAYVMLDVAARGRDVRAFVAHLEAWIIQALAAFNVEGGMRDGRVGVWVDRTEPGGPPREDKIAAIGVRLKRWVS
FHGISLNVEPELDHFTGITPCGIADPRYGVTSLADLGRIISMEEADIALKAAFQQVFASPLVRVDPPVTGSVPA
>Mature_234_residues
MQNFPPVEWAVSDAPVPYEAALAVMEARARAIREDGAPELVWFLEHPPLYTAGTSARIEDLKDPARFPVFEAGRGGQYTY
HGPGQRVAYVMLDVAARGRDVRAFVAHLEAWIIQALAAFNVEGGMRDGRVGVWVDRTEPGGPPREDKIAAIGVRLKRWVS
FHGISLNVEPELDHFTGITPCGIADPRYGVTSLADLGRIISMEEADIALKAAFQQVFASPLVRVDPPVTGSVPA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=195, Percent_Identity=34.8717948717949, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI87081767, Length=168, Percent_Identity=38.0952380952381, Blast_Score=122, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355483, Length=214, Percent_Identity=34.5794392523364, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_HYPNA (Q0C3A9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759784.1
- ProteinModelPortal:   Q0C3A9
- SMR:   Q0C3A9
- STRING:   Q0C3A9
- GeneID:   4288819
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1062
- NMPDR:   fig|228405.5.peg.3418
- TIGR:   HNE_1062
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q0C3A9
- BioCyc:   HNEP81032:HNE_1062-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25468; Mature: 25468

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFPPVEWAVSDAPVPYEAALAVMEARARAIREDGAPELVWFLEHPPLYTAGTSARIED
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHH
LKDPARFPVFEAGRGGQYTYHGPGQRVAYVMLDVAARGRDVRAFVAHLEAWIIQALAAFN
CCCCCCCCEEECCCCCCEEECCCCCEEEHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHHC
VEGGMRDGRVGVWVDRTEPGGPPREDKIAAIGVRLKRWVSFHGISLNVEPELDHFTGITP
CCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHCCCCC
CGIADPRYGVTSLADLGRIISMEEADIALKAAFQQVFASPLVRVDPPVTGSVPA
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
>Mature Secondary Structure
MQNFPPVEWAVSDAPVPYEAALAVMEARARAIREDGAPELVWFLEHPPLYTAGTSARIED
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHH
LKDPARFPVFEAGRGGQYTYHGPGQRVAYVMLDVAARGRDVRAFVAHLEAWIIQALAAFN
CCCCCCCCEEECCCCCCEEECCCCCEEEHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHHC
VEGGMRDGRVGVWVDRTEPGGPPREDKIAAIGVRLKRWVSFHGISLNVEPELDHFTGITP
CCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHCCCCC
CGIADPRYGVTSLADLGRIISMEEADIALKAAFQQVFASPLVRVDPPVTGSVPA
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA