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The map label for this gene is nylB' [H]

Identifier: 114799828

GI number: 114799828

Start: 1065676

End: 1066896

Strand: Reverse

Name: nylB' [H]

Synonym: HNE_1057

Alternate gene names: 114799828

Gene position: 1066896-1065676 (Counterclockwise)

Preceding gene: 114800027

Following gene: 114797915

Centisome position: 28.8

GC content: 66.26

Gene sequence:

>1221_bases
ATGAGCGGCACACAGAAATGGGTCCGGCGCATCGGGATTGTCGGGGTTGTCCTCGTCCTTGTCCTGGGCGCCACATGGAC
GCTGATCGGCCCGGATTGGCGCGCCCTCCTCGCCCATCCGCCCTCTGGCCGCGACATCCTCTTCTGGTCAAATCCCCAGC
GCGACGCGGCCTTCCGGATGATGGACCGCCTGCCGCTCATCATCGAATCGCGCCCCATCCCGAAGGGCAGCGCCACCCGC
CCCCTTCCCGAAGGCGCCCCGCTGGACCTCTCCGCCCAGCTCGACCTTGAAAGCTATCTTGCCACCCAGAACACCGCCGC
CCTCGTCATCCTGCAGGACGGCAAAATCCGCCTCGAGCGCTATGGCAATGCGTTCACCCCCGAAGGCCGCTGGACCAGCT
TCTCCGTCGCCAAATCCCTCACCTCCACCCTCGTCGGCGCCGCCATCGTGGATGGCCATATCGACAGCCTCAACGACCCC
GTCACGAAATACATCCCCGATCTGGCCGGCTCCCCCTATGACGACGTTACCATCGCCCAGCTCCTCACCATGACCTCCGG
CGTCGCCTGGAACGAAGATTATGAAGACCCGAATTCCGACGTCGCCCGCTTCGACAGCGCCGAGCCCGAGCCGGGCAAGA
GCAGCATCGTCACCTATATGCGCGGCCTCACGCGCGCCCATCCCCCCGGCGACGTCTGGAACTACTCGACCGGCGAGACC
AACCTCATCGGCGTCCTCGTCAGCGAGGCCACCGGCAAGCCGCTCTCAAACTATCTCTCCGAAAAGATCTGGGCGCCCTA
TGGCATGGAGCAGGACGCCACCTGGCTGCTGGGCTCGGACGGCCATGAAATCTCCGGCTGCTGCATTCAGGCTTCCACGC
GCGACTTTGCCCGCTTCGGCCAGTTCATCCTCGACGGCGCAATGACGGACGCCGGCCCCATCGTCCCCGCAGACTGGCTC
CCCGCCGCCACCGTCAAACAGGCCGACATCGGCGAGCCGGGCTATGGCTACGGCTTCCAGTGGTGGACCTGGGACGAGGG
CGCCTTCATGGCCGACGGCATCTTCGGCCAGGGCATCTTCATCGACCCCGCCCGCAACCTCGTCATCGCCTCAAACGCCA
ACTGGACCAGCGCGCTCGGCCTGCGCGACGGCGAATGGCCCGCCCGCGCCGACTTCTACCGCAAGGTCCAGGCCGCCATA
GACGCCGAAGCGGCCGAATAG

Upstream 100 bases:

>100_bases
CATGCTTGCCCCTCCGGCAATTTGCTGCACCGCCCCTTGCCGCCAAACCGCGCGCAACTAAAGCTGACGCCCGATAAAAA
ATGACGGGAGGGTCAGGTTT

Downstream 100 bases:

>100_bases
GGCCCACAAGCACAACGCCACTTACCCCTTGTATGTCATCTTTTGCCCGGCCTGCTATATTTCCGCGGTCGGCAGCGGAT
GAGAGCACCCCACCCCTGAG

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSGTQKWVRRIGIVGVVLVLVLGATWTLIGPDWRALLAHPPSGRDILFWSNPQRDAAFRMMDRLPLIIESRPIPKGSATR
PLPEGAPLDLSAQLDLESYLATQNTAALVILQDGKIRLERYGNAFTPEGRWTSFSVAKSLTSTLVGAAIVDGHIDSLNDP
VTKYIPDLAGSPYDDVTIAQLLTMTSGVAWNEDYEDPNSDVARFDSAEPEPGKSSIVTYMRGLTRAHPPGDVWNYSTGET
NLIGVLVSEATGKPLSNYLSEKIWAPYGMEQDATWLLGSDGHEISGCCIQASTRDFARFGQFILDGAMTDAGPIVPADWL
PAATVKQADIGEPGYGYGFQWWTWDEGAFMADGIFGQGIFIDPARNLVIASNANWTSALGLRDGEWPARADFYRKVQAAI
DAEAAE

Sequences:

>Translated_406_residues
MSGTQKWVRRIGIVGVVLVLVLGATWTLIGPDWRALLAHPPSGRDILFWSNPQRDAAFRMMDRLPLIIESRPIPKGSATR
PLPEGAPLDLSAQLDLESYLATQNTAALVILQDGKIRLERYGNAFTPEGRWTSFSVAKSLTSTLVGAAIVDGHIDSLNDP
VTKYIPDLAGSPYDDVTIAQLLTMTSGVAWNEDYEDPNSDVARFDSAEPEPGKSSIVTYMRGLTRAHPPGDVWNYSTGET
NLIGVLVSEATGKPLSNYLSEKIWAPYGMEQDATWLLGSDGHEISGCCIQASTRDFARFGQFILDGAMTDAGPIVPADWL
PAATVKQADIGEPGYGYGFQWWTWDEGAFMADGIFGQGIFIDPARNLVIASNANWTSALGLRDGEWPARADFYRKVQAAI
DAEAAE
>Mature_405_residues
SGTQKWVRRIGIVGVVLVLVLGATWTLIGPDWRALLAHPPSGRDILFWSNPQRDAAFRMMDRLPLIIESRPIPKGSATRP
LPEGAPLDLSAQLDLESYLATQNTAALVILQDGKIRLERYGNAFTPEGRWTSFSVAKSLTSTLVGAAIVDGHIDSLNDPV
TKYIPDLAGSPYDDVTIAQLLTMTSGVAWNEDYEDPNSDVARFDSAEPEPGKSSIVTYMRGLTRAHPPGDVWNYSTGETN
LIGVLVSEATGKPLSNYLSEKIWAPYGMEQDATWLLGSDGHEISGCCIQASTRDFARFGQFILDGAMTDAGPIVPADWLP
AATVKQADIGEPGYGYGFQWWTWDEGAFMADGIFGQGIFIDPARNLVIASNANWTSALGLRDGEWPARADFYRKVQAAID
AEAAE

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 44169; Mature: 44038

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTQKWVRRIGIVGVVLVLVLGATWTLIGPDWRALLAHPPSGRDILFWSNPQRDAAFRM
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCEEEEECCCCHHHHHHH
MDRLPLIIESRPIPKGSATRPLPEGAPLDLSAQLDLESYLATQNTAALVILQDGKIRLER
HHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCEEEEEEECCCEEEEE
YGNAFTPEGRWTSFSVAKSLTSTLVGAAIVDGHIDSLNDPVTKYIPDLAGSPYDDVTIAQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHCCCCCCCHHHHH
LLTMTSGVAWNEDYEDPNSDVARFDSAEPEPGKSSIVTYMRGLTRAHPPGDVWNYSTGET
HHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
NLIGVLVSEATGKPLSNYLSEKIWAPYGMEQDATWLLGSDGHEISGCCIQASTRDFARFG
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHH
QFILDGAMTDAGPIVPADWLPAATVKQADIGEPGYGYGFQWWTWDEGAFMADGIFGQGIF
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEE
IDPARNLVIASNANWTSALGLRDGEWPARADFYRKVQAAIDAEAAE
ECCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGTQKWVRRIGIVGVVLVLVLGATWTLIGPDWRALLAHPPSGRDILFWSNPQRDAAFRM
CCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCEEEEECCCCHHHHHHH
MDRLPLIIESRPIPKGSATRPLPEGAPLDLSAQLDLESYLATQNTAALVILQDGKIRLER
HHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCEEEEEEECCCEEEEE
YGNAFTPEGRWTSFSVAKSLTSTLVGAAIVDGHIDSLNDPVTKYIPDLAGSPYDDVTIAQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHCCCCCCCHHHHH
LLTMTSGVAWNEDYEDPNSDVARFDSAEPEPGKSSIVTYMRGLTRAHPPGDVWNYSTGET
HHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
NLIGVLVSEATGKPLSNYLSEKIWAPYGMEQDATWLLGSDGHEISGCCIQASTRDFARFG
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHH
QFILDGAMTDAGPIVPADWLPAATVKQADIGEPGYGYGFQWWTWDEGAFMADGIFGQGIF
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEE
IDPARNLVIASNANWTSALGLRDGEWPARADFYRKVQAAIDAEAAE
ECCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]