Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 114799693

GI number: 114799693

Start: 3197392

End: 3198828

Strand: Reverse

Name: murF [H]

Synonym: HNE_3028

Alternate gene names: 114799693

Gene position: 3198828-3197392 (Counterclockwise)

Preceding gene: 114798974

Following gene: 114797628

Centisome position: 86.34

GC content: 63.12

Gene sequence:

>1437_bases
ATGAACCGCCCCCTCTGGACTTCCAGCGACATGGAGCTTGCCACCGGCGGCGACGCCAGCGCGGCGTTTGAAGTGACGGG
ATCGATTTCGATCGATACGCGCACCCTGCAGAAGGGCGATCTGTTCGTGGCGTTGAAAGATCAGCGCGACGGGCATGATT
TCGTCGAGGCCGCTTTCAAGGCAGGCGCGGCGGGCGCGCTTGTTTCCCGTCCCATGCCGGGCGGACCGGTCGTGCAGGTT
GAGGATGTGCTTGAGGCGTTGACGCAGATGGGCATTGCGGCGCGGGTACGTTGCGGCGCGCATCGCACAGCGGTGACGGG
GTCCGCAGGCAAGACCTCGGTGAAGGAAATGCTGGCGCAGATCTATCGCGCGCATGGCCCGGCCCACTGGAACGTCAAGA
GCTTCAACAATCACTGGGGCGTGCCGCTGACGCTGGCCCGGATGCCGGAAGAAACGCAGCGCGCCGTCTTCGAGATCGGC
ATGAATACGCCCGGCGAGATTGCCCCGCGCAGCCAGATGGTGCGCCCGCATACCGGTATTGTGACCTGTATCGCTGCCGC
CCACCTGCAAGGGCTTGGCTCGGTCGAGGGGATTGCCGAAGAGAAAGCCAACATTTTTGCCGGGCTTGAGTCCGGCGGCA
CGATCATTCTGCCCGCGGATGATACCTTTTTAGACTATCTTTCCGAACGGGCACGGAGTTTCTGTCCGACAGGCAATGTT
GAAACATTCGGGGCGTCTCCGGACGCAACGGCCCGCATCGTGGCGTATGAAACCGATGGCCGCACCAGCCGCGTTGAGGT
CAGCATTGTGGGCCAGCGCGTCGCCGTCACGCTGAACGCCGTCGGCGCTCACTGGGCGCAGAATGTCGCTGCTGCCTTGC
TGGCGGCAAGCCAGGCCAGCCTGTCGGTCAAGGATTGCGCTGAGGCGCTGTCTGGTTACGCGCCACCGCCCGGCCGGGGC
ACTTCCGAAACGCTGGTTTTGCCGGATATCGGCGAAATCACGCTGATTGATGACGCCTACAACGCCAATCCTGCCAGCAT
GCGTGCGGCGCTCAAGGGCCTGGCTCAACGCGCTGGCACGCGCCGCCTAGCGGCGCTGGGCGATATGAAGGAAATCGGGG
ATTCCTCGGCCGCAGAACACAGGGGGCTTGCCAGTTCCGTGGTGGAAGCGGGCGTTGACGGTGTGTTCCTGGTTGGTGCT
GAAATGCAACACCTTTCCGAGGCGCTTCCGCGCGATCTGCAGCAGGTATCTGCTCCGACAGCGGATGAGTTGTGGAATCA
ACTGCAAAAAGCACTCCGCGCTGGGGATGTTCTCTTGATCAAAGGGTCGAATGCGTCCGGGATGGGTCGAATAGCGGACC
GGCTGCGCCAATGGAGCGTGCCGGCCGGATTGGGCAAGATGGATGGCGGTCCAGAAGGGGCTGCGAGGGTAAGCTGA

Upstream 100 bases:

>100_bases
TCGCCGGCAAGGGACATGAGACCGGCCAGATCATCGGTTCGCAGGTTCTGCCCTTCTCCGATCAGGATGAGGCGCTGGCC
GCCCTGAAAGGGCTTGTGCG

Downstream 100 bases:

>100_bases
TGCTGTACGAACTGCTGGCCGCAGACAGCGGTCTCTTCAACCTGCTGAACTACATCACCTTCCGCACAGGTGCGGCGGTC
GTCACTGCATTTCTGGTGAC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNRPLWTSSDMELATGGDASAAFEVTGSISIDTRTLQKGDLFVALKDQRDGHDFVEAAFKAGAAGALVSRPMPGGPVVQV
EDVLEALTQMGIAARVRCGAHRTAVTGSAGKTSVKEMLAQIYRAHGPAHWNVKSFNNHWGVPLTLARMPEETQRAVFEIG
MNTPGEIAPRSQMVRPHTGIVTCIAAAHLQGLGSVEGIAEEKANIFAGLESGGTIILPADDTFLDYLSERARSFCPTGNV
ETFGASPDATARIVAYETDGRTSRVEVSIVGQRVAVTLNAVGAHWAQNVAAALLAASQASLSVKDCAEALSGYAPPPGRG
TSETLVLPDIGEITLIDDAYNANPASMRAALKGLAQRAGTRRLAALGDMKEIGDSSAAEHRGLASSVVEAGVDGVFLVGA
EMQHLSEALPRDLQQVSAPTADELWNQLQKALRAGDVLLIKGSNASGMGRIADRLRQWSVPAGLGKMDGGPEGAARVS

Sequences:

>Translated_478_residues
MNRPLWTSSDMELATGGDASAAFEVTGSISIDTRTLQKGDLFVALKDQRDGHDFVEAAFKAGAAGALVSRPMPGGPVVQV
EDVLEALTQMGIAARVRCGAHRTAVTGSAGKTSVKEMLAQIYRAHGPAHWNVKSFNNHWGVPLTLARMPEETQRAVFEIG
MNTPGEIAPRSQMVRPHTGIVTCIAAAHLQGLGSVEGIAEEKANIFAGLESGGTIILPADDTFLDYLSERARSFCPTGNV
ETFGASPDATARIVAYETDGRTSRVEVSIVGQRVAVTLNAVGAHWAQNVAAALLAASQASLSVKDCAEALSGYAPPPGRG
TSETLVLPDIGEITLIDDAYNANPASMRAALKGLAQRAGTRRLAALGDMKEIGDSSAAEHRGLASSVVEAGVDGVFLVGA
EMQHLSEALPRDLQQVSAPTADELWNQLQKALRAGDVLLIKGSNASGMGRIADRLRQWSVPAGLGKMDGGPEGAARVS
>Mature_478_residues
MNRPLWTSSDMELATGGDASAAFEVTGSISIDTRTLQKGDLFVALKDQRDGHDFVEAAFKAGAAGALVSRPMPGGPVVQV
EDVLEALTQMGIAARVRCGAHRTAVTGSAGKTSVKEMLAQIYRAHGPAHWNVKSFNNHWGVPLTLARMPEETQRAVFEIG
MNTPGEIAPRSQMVRPHTGIVTCIAAAHLQGLGSVEGIAEEKANIFAGLESGGTIILPADDTFLDYLSERARSFCPTGNV
ETFGASPDATARIVAYETDGRTSRVEVSIVGQRVAVTLNAVGAHWAQNVAAALLAASQASLSVKDCAEALSGYAPPPGRG
TSETLVLPDIGEITLIDDAYNANPASMRAALKGLAQRAGTRRLAALGDMKEIGDSSAAEHRGLASSVVEAGVDGVFLVGA
EMQHLSEALPRDLQQVSAPTADELWNQLQKALRAGDVLLIKGSNASGMGRIADRLRQWSVPAGLGKMDGGPEGAARVS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=444, Percent_Identity=35.1351351351351, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49975; Mature: 49975

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPLWTSSDMELATGGDASAAFEVTGSISIDTRTLQKGDLFVALKDQRDGHDFVEAAFK
CCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCEEECCCCEEEEEECCCCCHHHHHHHHH
AGAAGALVSRPMPGGPVVQVEDVLEALTQMGIAARVRCGAHRTAVTGSAGKTSVKEMLAQ
CCCCCHHHCCCCCCCCEEEHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCHHHHHHHHHH
IYRAHGPAHWNVKSFNNHWGVPLTLARMPEETQRAVFEIGMNTPGEIAPRSQMVRPHTGI
HHHHCCCCCCEECCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
VTCIAAAHLQGLGSVEGIAEEKANIFAGLESGGTIILPADDTFLDYLSERARSFCPTGNV
HHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCC
ETFGASPDATARIVAYETDGRTSRVEVSIVGQRVAVTLNAVGAHWAQNVAAALLAASQAS
EEECCCCCCCEEEEEEECCCCCEEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHCC
LSVKDCAEALSGYAPPPGRGTSETLVLPDIGEITLIDDAYNANPASMRAALKGLAQRAGT
CCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
RRLAALGDMKEIGDSSAAEHRGLASSVVEAGVDGVFLVGAEMQHLSEALPRDLQQVSAPT
HHHHHHCCHHHHCCCCHHHHCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCC
ADELWNQLQKALRAGDVLLIKGSNASGMGRIADRLRQWSVPAGLGKMDGGPEGAARVS
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRPLWTSSDMELATGGDASAAFEVTGSISIDTRTLQKGDLFVALKDQRDGHDFVEAAFK
CCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCEEECCCCEEEEEECCCCCHHHHHHHHH
AGAAGALVSRPMPGGPVVQVEDVLEALTQMGIAARVRCGAHRTAVTGSAGKTSVKEMLAQ
CCCCCHHHCCCCCCCCEEEHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCHHHHHHHHHH
IYRAHGPAHWNVKSFNNHWGVPLTLARMPEETQRAVFEIGMNTPGEIAPRSQMVRPHTGI
HHHHCCCCCCEECCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
VTCIAAAHLQGLGSVEGIAEEKANIFAGLESGGTIILPADDTFLDYLSERARSFCPTGNV
HHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCC
ETFGASPDATARIVAYETDGRTSRVEVSIVGQRVAVTLNAVGAHWAQNVAAALLAASQAS
EEECCCCCCCEEEEEEECCCCCEEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHCC
LSVKDCAEALSGYAPPPGRGTSETLVLPDIGEITLIDDAYNANPASMRAALKGLAQRAGT
CCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
RRLAALGDMKEIGDSSAAEHRGLASSVVEAGVDGVFLVGAEMQHLSEALPRDLQQVSAPT
HHHHHHCCHHHHCCCCHHHHCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCC
ADELWNQLQKALRAGDVLLIKGSNASGMGRIADRLRQWSVPAGLGKMDGGPEGAARVS
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]