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The map label for this gene is pspF [H]

Identifier: 114799167

GI number: 114799167

Start: 3455430

End: 3456536

Strand: Reverse

Name: pspF [H]

Synonym: HNE_3292

Alternate gene names: 114799167

Gene position: 3456536-3455430 (Counterclockwise)

Preceding gene: 114799736

Following gene: 114797169

Centisome position: 93.29

GC content: 65.67

Gene sequence:

>1107_bases
ATGGAGCCGCGCACAACGCAACTGATCGGGGAATCGGCCGCCTGGCTGAGCGCGCTCGAGCAGGCCAGCCGGGTCGCGCC
GCTGGACCGGTCGGTGCTGGTGATCGGGGAGCGCGGCACCGGCAAGGAGCTGGTCGGTGAGCGGCTGCACTTCCTGTCAA
AACGCTGGGAAGGCCCGTTCATCAAGGTGAACTGCGCGGCGCTGTCCGAGACGCTGCTGGATTCCGAACTGTTCGGCCAT
GAGCGCGGCGCGTTTACCGGCGCCACCGACATGCGGCGGGGCCGTTTCGAGATGGCCGACGGGGGCACGATCTTCCTCGA
CGAGATCGCGACGGCCTCCCAGCAGGTGCAGGAAAAGCTGCTGAGGATCGTGGAATACGGGGAATTTCAACGGGTTGGCG
GCTCCAAGGTGCTCTCGACCAATGTGCGGATCGTGGCGGCGACCAATGCCGATCTGCCGGCGCTGGCGGAGGCGGGGGCG
TTCCGCTGGGATCTGCTGGACCGGTTGTCATTCGACGTGATCACGCTGCCCCCGCTACGGGCCCGGCAGGGGGACAAGAC
GCTGCTGGCGCAGTTCTTTGCGCGGCGTATGGCGGTTGAAATGGCTCAGGATTTTCCGGGATTTGCGCCCTCTGCGCTGG
AGGCCATCGAGGCCTGGCACTGGCCGGGAAACATCCGGGAGCTGCGCAATTTTGCCGAGCGGCTGACCCATCGGGCGCTG
ATCTCTGCGCCGTTTGAGCAGGTGCGGTTTGAGACGTCCGCGCTTGATCCGTTCGCCTCGCCCTGGCGGCCGAAAAACGC
CCCGAAAAGCTCCCCAAAAAACTCCAACGAAACCTCCAACGAGGCAGGCAAAAACTCCAACGGCGTTGGCGTTTTTCTGG
GCGCGTCGAGCCCTTCCAGCGCCGCCGAGCTTCAACCCGCCGAGCGGCCCCAAAGCCCGGCGCGCGGCTTTGAAGACGCG
GTGCGTCTTTTCGAAATGCAACTGATCGATGGCGCGCTGGCCGATGCCGGAGGCCATCAGGGCAGGGCGGCCGACGCCCT
CGGCCTGACCTATCACCAGCTGCGCGGCCTGCTGAAAAAGCATGGATATGCCAAGCGGGCGGACTGA

Upstream 100 bases:

>100_bases
ATGATAAAAATGTTTCCGGCGCGCTGGCGTTAGGAAATTAAAGGTCAAATATCCAATCTACTTAGTGGATTTGACCAATG
AAATTGGCTAAACAAACCAT

Downstream 100 bases:

>100_bases
ATCTGGGCTCAGAAATGGCCGATCAAAGGTTGGCGGTGGGCGATCCTGCCGTTACCATTCGCCGGTGGCCATTTCGGGGG
GAAGCCGATGATGAATGCCT

Product: psp operon transcription activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MEPRTTQLIGESAAWLSALEQASRVAPLDRSVLVIGERGTGKELVGERLHFLSKRWEGPFIKVNCAALSETLLDSELFGH
ERGAFTGATDMRRGRFEMADGGTIFLDEIATASQQVQEKLLRIVEYGEFQRVGGSKVLSTNVRIVAATNADLPALAEAGA
FRWDLLDRLSFDVITLPPLRARQGDKTLLAQFFARRMAVEMAQDFPGFAPSALEAIEAWHWPGNIRELRNFAERLTHRAL
ISAPFEQVRFETSALDPFASPWRPKNAPKSSPKNSNETSNEAGKNSNGVGVFLGASSPSSAAELQPAERPQSPARGFEDA
VRLFEMQLIDGALADAGGHQGRAADALGLTYHQLRGLLKKHGYAKRAD

Sequences:

>Translated_368_residues
MEPRTTQLIGESAAWLSALEQASRVAPLDRSVLVIGERGTGKELVGERLHFLSKRWEGPFIKVNCAALSETLLDSELFGH
ERGAFTGATDMRRGRFEMADGGTIFLDEIATASQQVQEKLLRIVEYGEFQRVGGSKVLSTNVRIVAATNADLPALAEAGA
FRWDLLDRLSFDVITLPPLRARQGDKTLLAQFFARRMAVEMAQDFPGFAPSALEAIEAWHWPGNIRELRNFAERLTHRAL
ISAPFEQVRFETSALDPFASPWRPKNAPKSSPKNSNETSNEAGKNSNGVGVFLGASSPSSAAELQPAERPQSPARGFEDA
VRLFEMQLIDGALADAGGHQGRAADALGLTYHQLRGLLKKHGYAKRAD
>Mature_368_residues
MEPRTTQLIGESAAWLSALEQASRVAPLDRSVLVIGERGTGKELVGERLHFLSKRWEGPFIKVNCAALSETLLDSELFGH
ERGAFTGATDMRRGRFEMADGGTIFLDEIATASQQVQEKLLRIVEYGEFQRVGGSKVLSTNVRIVAATNADLPALAEAGA
FRWDLLDRLSFDVITLPPLRARQGDKTLLAQFFARRMAVEMAQDFPGFAPSALEAIEAWHWPGNIRELRNFAERLTHRAL
ISAPFEQVRFETSALDPFASPWRPKNAPKSSPKNSNETSNEAGKNSNGVGVFLGASSPSSAAELQPAERPQSPARGFEDA
VRLFEMQLIDGALADAGGHQGRAADALGLTYHQLRGLLKKHGYAKRAD

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=356, Percent_Identity=50, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1788550, Length=228, Percent_Identity=48.2456140350877, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI87082117, Length=227, Percent_Identity=48.0176211453744, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1790437, Length=227, Percent_Identity=48.8986784140969, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789087, Length=228, Percent_Identity=45.6140350877193, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1789233, Length=237, Percent_Identity=44.3037974683544, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87082152, Length=240, Percent_Identity=44.5833333333333, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1790299, Length=232, Percent_Identity=45.6896551724138, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788905, Length=237, Percent_Identity=42.6160337552743, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=39.7590361445783, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787583, Length=229, Percent_Identity=36.6812227074236, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789828, Length=227, Percent_Identity=35.2422907488987, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081858, Length=204, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40266; Mature: 40266

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPRTTQLIGESAAWLSALEQASRVAPLDRSVLVIGERGTGKELVGERLHFLSKRWEGPF
CCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCE
IKVNCAALSETLLDSELFGHERGAFTGATDMRRGRFEMADGGTIFLDEIATASQQVQEKL
EEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHH
LRIVEYGEFQRVGGSKVLSTNVRIVAATNADLPALAEAGAFRWDLLDRLSFDVITLPPLR
HHHHHCCCHHHCCCCEEEECCEEEEEECCCCCCHHHHCCCHHHHHHHHCCCCEEECCCCC
ARQGDKTLLAQFFARRMAVEMAQDFPGFAPSALEAIEAWHWPGNIRELRNFAERLTHRAL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ISAPFEQVRFETSALDPFASPWRPKNAPKSSPKNSNETSNEAGKNSNGVGVFLGASSPSS
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCC
AAELQPAERPQSPARGFEDAVRLFEMQLIDGALADAGGHQGRAADALGLTYHQLRGLLKK
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
HGYAKRAD
CCCCCCCC
>Mature Secondary Structure
MEPRTTQLIGESAAWLSALEQASRVAPLDRSVLVIGERGTGKELVGERLHFLSKRWEGPF
CCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCE
IKVNCAALSETLLDSELFGHERGAFTGATDMRRGRFEMADGGTIFLDEIATASQQVQEKL
EEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHH
LRIVEYGEFQRVGGSKVLSTNVRIVAATNADLPALAEAGAFRWDLLDRLSFDVITLPPLR
HHHHHCCCHHHCCCCEEEECCEEEEEECCCCCCHHHHCCCHHHHHHHHCCCCEEECCCCC
ARQGDKTLLAQFFARRMAVEMAQDFPGFAPSALEAIEAWHWPGNIRELRNFAERLTHRAL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ISAPFEQVRFETSALDPFASPWRPKNAPKSSPKNSNETSNEAGKNSNGVGVFLGASSPSS
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCC
AAELQPAERPQSPARGFEDAVRLFEMQLIDGALADAGGHQGRAADALGLTYHQLRGLLKK
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
HGYAKRAD
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]