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The map label for this gene is mnmC

Identifier: 114799736

GI number: 114799736

Start: 3458613

End: 3460433

Strand: Reverse

Name: mnmC

Synonym: HNE_3298

Alternate gene names: 114799736

Gene position: 3460433-3458613 (Counterclockwise)

Preceding gene: 114797411

Following gene: 114799167

Centisome position: 93.4

GC content: 67.66

Gene sequence:

>1821_bases
ATGACACGGCTGCTGACTCATCCGGACATTGCGTGGCGGGAGGATGGTACGCCTTTCGCCACCGCGTTTGGTGACGTGTA
TTTCTCCGCGCAGGATGGGCTGGCTGAGACGCGGGCGGTATTTTTCAAAGGCTGCGGGCTGCCTGAAGCCTGGGCCGGGC
GGGCGCAGTTCACCGTGGCCGAGACCGGCTTTGGGACGGGGCTGAATTTCCTGGCGCTGTGGCAGCTTTGGCGCGAACAC
CGGCCCCATCCGGCGGCGCGGCTCAGCTTTGTCAGTTTTGAGGGCTATCCCCTGAGGGCGGAGGACGCGGCGCGCGCCTT
TGCGGCCTGGCCGGAACTTTCCCCTCTGGCTGACCAGCTGCTGGCGCGGTGGCCGGGGCCGGTCCGGGGCGTACGGCATC
TCGCCTTTGAAGCAGACGGGATCGAGCTGATCCTGCATCTGGGGGATATTGCGCAGACATTGCCCGCGAGCGGCTTTCGC
GCCGATGCCTGGTTTCTGGACGGGTTCAGCCCGGCGAAGAATGCCGGGATGTGGGCAGGCGATATTTTCCCGCTCATTGC
AGCCCGGTCTGCGCCCGGCGCGCGGGCGGCGACGTTTACCGTGGCCGGTGATGTGCGCCGGGGACTGGCAGAGGCAGGAT
TTGAGGTGCGCAAGGCCGAAGGCCATGGCCGCAAGCGCCAGCGACTGGAGGCGGTTTATCCGGATGCGGATGAGCCCGTG
ATCCCGCTTCCGCGCCGTGTGGCGATCCTCGGGGCCGGAATAGCGGGAGCAACAATCGCGTCCGGGCTGAAACTGGCGGG
CATGGAGGCCGTGGTGTTCGATCCCGCGCCTGAACCTGCGCGCGGGGCGAGCGGCAATGCGCTGGCGTTGATGATGCCGC
GCCTGGACGCAGGCGATACCGCCGAAGCGCGCCTGCTGATCGATGCCTATCTGGCCGCGCGGGACTTTTATCTGGGGCGT
CCCGGCGCGGAGCTGACTGATGTGCGCCAGATGCCGCGCAATGAGACCGAAGAGAAACGGTTCGGTAAGCTGCTCGCCGA
TCCACCCTTTCCGCTGGAGGACCTCGAAGCTCTTTCCGGCGGCGGCATTCTGCACAAGCGTGCCCTGATCCTCCGACCCC
GGCAGATGGTTCTGTCGCTGCTGGACGGGGTTGAGGTGCGCGCTGGCGCGATGCCGGATGTGGACCTTGGGGCGCGCAGC
GTAAACGGTGAGGTATTTGATGCCATCGTGCTGGCGAGCGGCATGGGTATGGGTCAGCTCCTGCCCGCGCTGGAGCTGGC
AGGGCGTCAGGGGCAGGTGGAGCATCTTTCCGGCGCCGTGCAGGCCCCGCCCTCGGCGCTGGCATCGGGCACGTATGCGA
TTGCTCTGGGAAATGAGCGCCTCTGGGGCGCCACCTTCGAGCCGGCTGGCGAGGGCGTAAGCGCCGCTGTCAGTGAGGCG
GCGCGCGCGGAAAATCTTGAAGGCGTTGAGGCGCTTTCGCCCTGGTGGGTGCGGGAGGCGCAGGGCGGCGCGGCCGTGTC
GCGCGCTTCGGTGCGGGCGACAACGCCGGACAGGCTGCCCCTGATCGGCGCAGCGCCGGACGCGGGTGCCATAGCGGCGC
TGTGGGACGGAAAGGCGGAGCGGGCGTCGTCGCCCGTCCTGCCGGGGGTCTATGTTGCGGGAGGCTTTGGCAGCCGGGGC
TTTACATGGGCGCCCTGGGCTGCTGCGATCATTGCTGCCAAGCTCTGTAATGGCCCGCCGCCGGCTGATCTGAATTCCCT
GAGGGCTGTTGACCCTGCACGGCAGATCCTCCGGCGTCTGAGGAAGACCGGCAGGCTTTAG

Upstream 100 bases:

>100_bases
GGGCAGAAAAGCGCAATGCCCGGTCTGGTCCCTTATCCGGTTGCTGGCCCGATTGCTGGCCCGGTTTGCATTTTTGCGTG
GCCGGGCTAGGGCAGGGCGC

Downstream 100 bases:

>100_bases
GGCGCCTGCACGAAAAAGACGCGCACCCCGCCATTTTCATGACGTTCCAGGCTCATGGAAACGCCCGCAAGTTCGAGGGA
TGCGTTGCCGGCAACCGTGC

Product: FAD dependent oxidoreductase domain-containing protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MTRLLTHPDIAWREDGTPFATAFGDVYFSAQDGLAETRAVFFKGCGLPEAWAGRAQFTVAETGFGTGLNFLALWQLWREH
RPHPAARLSFVSFEGYPLRAEDAARAFAAWPELSPLADQLLARWPGPVRGVRHLAFEADGIELILHLGDIAQTLPASGFR
ADAWFLDGFSPAKNAGMWAGDIFPLIAARSAPGARAATFTVAGDVRRGLAEAGFEVRKAEGHGRKRQRLEAVYPDADEPV
IPLPRRVAILGAGIAGATIASGLKLAGMEAVVFDPAPEPARGASGNALALMMPRLDAGDTAEARLLIDAYLAARDFYLGR
PGAELTDVRQMPRNETEEKRFGKLLADPPFPLEDLEALSGGGILHKRALILRPRQMVLSLLDGVEVRAGAMPDVDLGARS
VNGEVFDAIVLASGMGMGQLLPALELAGRQGQVEHLSGAVQAPPSALASGTYAIALGNERLWGATFEPAGEGVSAAVSEA
ARAENLEGVEALSPWWVREAQGGAAVSRASVRATTPDRLPLIGAAPDAGAIAALWDGKAERASSPVLPGVYVAGGFGSRG
FTWAPWAAAIIAAKLCNGPPPADLNSLRAVDPARQILRRLRKTGRL

Sequences:

>Translated_606_residues
MTRLLTHPDIAWREDGTPFATAFGDVYFSAQDGLAETRAVFFKGCGLPEAWAGRAQFTVAETGFGTGLNFLALWQLWREH
RPHPAARLSFVSFEGYPLRAEDAARAFAAWPELSPLADQLLARWPGPVRGVRHLAFEADGIELILHLGDIAQTLPASGFR
ADAWFLDGFSPAKNAGMWAGDIFPLIAARSAPGARAATFTVAGDVRRGLAEAGFEVRKAEGHGRKRQRLEAVYPDADEPV
IPLPRRVAILGAGIAGATIASGLKLAGMEAVVFDPAPEPARGASGNALALMMPRLDAGDTAEARLLIDAYLAARDFYLGR
PGAELTDVRQMPRNETEEKRFGKLLADPPFPLEDLEALSGGGILHKRALILRPRQMVLSLLDGVEVRAGAMPDVDLGARS
VNGEVFDAIVLASGMGMGQLLPALELAGRQGQVEHLSGAVQAPPSALASGTYAIALGNERLWGATFEPAGEGVSAAVSEA
ARAENLEGVEALSPWWVREAQGGAAVSRASVRATTPDRLPLIGAAPDAGAIAALWDGKAERASSPVLPGVYVAGGFGSRG
FTWAPWAAAIIAAKLCNGPPPADLNSLRAVDPARQILRRLRKTGRL
>Mature_605_residues
TRLLTHPDIAWREDGTPFATAFGDVYFSAQDGLAETRAVFFKGCGLPEAWAGRAQFTVAETGFGTGLNFLALWQLWREHR
PHPAARLSFVSFEGYPLRAEDAARAFAAWPELSPLADQLLARWPGPVRGVRHLAFEADGIELILHLGDIAQTLPASGFRA
DAWFLDGFSPAKNAGMWAGDIFPLIAARSAPGARAATFTVAGDVRRGLAEAGFEVRKAEGHGRKRQRLEAVYPDADEPVI
PLPRRVAILGAGIAGATIASGLKLAGMEAVVFDPAPEPARGASGNALALMMPRLDAGDTAEARLLIDAYLAARDFYLGRP
GAELTDVRQMPRNETEEKRFGKLLADPPFPLEDLEALSGGGILHKRALILRPRQMVLSLLDGVEVRAGAMPDVDLGARSV
NGEVFDAIVLASGMGMGQLLPALELAGRQGQVEHLSGAVQAPPSALASGTYAIALGNERLWGATFEPAGEGVSAAVSEAA
RAENLEGVEALSPWWVREAQGGAAVSRASVRATTPDRLPLIGAAPDAGAIAALWDGKAERASSPVLPGVYVAGGFGSRGF
TWAPWAAAIIAAKLCNGPPPADLNSLRAVDPARQILRRLRKTGRL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=661, Percent_Identity=33.1316187594554, Blast_Score=279, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_HYPNA (Q0BX22)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761971.1
- ProteinModelPortal:   Q0BX22
- SMR:   Q0BX22
- STRING:   Q0BX22
- GeneID:   4288020
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3298
- NMPDR:   fig|228405.5.peg.3158
- TIGR:   HNE_3298
- eggNOG:   COG4121
- HOGENOM:   HBG677640
- OMA:   ARDDNPR
- PhylomeDB:   Q0BX22
- ProtClustDB:   CLSK2532043
- BioCyc:   HNEP81032:HNE_3298-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 64095; Mature: 63964

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLLTHPDIAWREDGTPFATAFGDVYFSAQDGLAETRAVFFKGCGLPEAWAGRAQFTVA
CCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHCCCCCHHHCCCEEEEEE
ETGFGTGLNFLALWQLWREHRPHPAARLSFVSFEGYPLRAEDAARAFAAWPELSPLADQL
ECCCCCCHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHH
LARWPGPVRGVRHLAFEADGIELILHLGDIAQTLPASGFRADAWFLDGFSPAKNAGMWAG
HHHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCH
DIFPLIAARSAPGARAATFTVAGDVRRGLAEAGFEVRKAEGHGRKRQRLEAVYPDADEPV
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCC
IPLPRRVAILGAGIAGATIASGLKLAGMEAVVFDPAPEPARGASGNALALMMPRLDAGDT
CCCCCEEEEEECCHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
AEARLLIDAYLAARDFYLGRPGAELTDVRQMPRNETEEKRFGKLLADPPFPLEDLEALSG
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHCC
GGILHKRALILRPRQMVLSLLDGVEVRAGAMPDVDLGARSVNGEVFDAIVLASGMGMGQL
CCHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
LPALELAGRQGQVEHLSGAVQAPPSALASGTYAIALGNERLWGATFEPAGEGVSAAVSEA
HHHHHHCCCCCCHHHHCCCCCCCHHHHCCCEEEEEECCCCEECCCCCCCCCCHHHHHHHH
ARAENLEGVEALSPWWVREAQGGAAVSRASVRATTPDRLPLIGAAPDAGAIAALWDGKAE
HHHCCCCCHHHCCCHHHEECCCCCCEECCEEECCCCCCCCEEECCCCCCCEEEEECCCHH
RASSPVLPGVYVAGGFGSRGFTWAPWAAAIIAAKLCNGPPPADLNSLRAVDPARQILRRL
HCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHH
RKTGRL
HHHCCC
>Mature Secondary Structure 
TRLLTHPDIAWREDGTPFATAFGDVYFSAQDGLAETRAVFFKGCGLPEAWAGRAQFTVA
CCCCCCCCCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHCCCCCHHHCCCEEEEEE
ETGFGTGLNFLALWQLWREHRPHPAARLSFVSFEGYPLRAEDAARAFAAWPELSPLADQL
ECCCCCCHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHH
LARWPGPVRGVRHLAFEADGIELILHLGDIAQTLPASGFRADAWFLDGFSPAKNAGMWAG
HHHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCH
DIFPLIAARSAPGARAATFTVAGDVRRGLAEAGFEVRKAEGHGRKRQRLEAVYPDADEPV
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCC
IPLPRRVAILGAGIAGATIASGLKLAGMEAVVFDPAPEPARGASGNALALMMPRLDAGDT
CCCCCEEEEEECCHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCC
AEARLLIDAYLAARDFYLGRPGAELTDVRQMPRNETEEKRFGKLLADPPFPLEDLEALSG
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHCC
GGILHKRALILRPRQMVLSLLDGVEVRAGAMPDVDLGARSVNGEVFDAIVLASGMGMGQL
CCHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
LPALELAGRQGQVEHLSGAVQAPPSALASGTYAIALGNERLWGATFEPAGEGVSAAVSEA
HHHHHHCCCCCCHHHHCCCCCCCHHHHCCCEEEEEECCCCEECCCCCCCCCCHHHHHHHH
ARAENLEGVEALSPWWVREAQGGAAVSRASVRATTPDRLPLIGAAPDAGAIAALWDGKAE
HHHCCCCCHHHCCCHHHEECCCCCCEECCEEECCCCCCCCEEECCCCCCCEEEEECCCHH
RASSPVLPGVYVAGGFGSRGFTWAPWAAAIIAAKLCNGPPPADLNSLRAVDPARQILRRL
HCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHH
RKTGRL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA