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The map label for this gene is murE [H]

Identifier: 114798974

GI number: 114798974

Start: 3198825

End: 3200261

Strand: Reverse

Name: murE [H]

Synonym: HNE_3029

Alternate gene names: 114798974

Gene position: 3200261-3198825 (Counterclockwise)

Preceding gene: 114798338

Following gene: 114799693

Centisome position: 86.38

GC content: 64.65

Gene sequence:

>1437_bases
TTGAAACTCTCGGACCTTTTCCCCCTCGCCGAGAATGGCGAGACCGAGATACTCGGTATGACGTCAGACAGCCGGAAGGT
GCAGCCTGGCTGGCTGTTTGCGGCGCTCAAAGGCAGCGTGCATGACGGGGGCAAGTTTGTGCCCCAGGCCGTGGCGCAGG
GCGCCGCCGCAATTCTTTCCGATGGCGGCATCGGCGCCGTATCTGTGCCGCATGTGATGGCTGATGAGCCGCGCCGCGCG
CTGGCGCTGGCGGCCAAGCGGTTCTATGGCGAGCAGCCTGAATTCGTCGTCGCGGTGACCGGCACCAATGGCAAATCCTC
GACCGTGGATTTTGCCCGCCAGATATGGAGCCGGGCGGGCATCAAGGCCGCTTCGATGGGCACGCTCGGCGCGATTGGCC
CGGCCGGCAAGGTGGACATCGGGCATACGACGCCCGATCCCGTGACCGTCAGTGAAACGATGAAAGCGCTGGCCCGGCAG
GGCGTGACACATTGTGCGATGGAAGCCTCCAGCCACGGCCTGGAGCAGCACCGCCTCGACGGCGTTGATCTGTCCGCCGT
GGCGTTTCTCAATCTGACGCAGGACCATCTCGACTATCACGCGACGATGGAAGCCTATCTGGACGCCAAGGTGCGCCTCT
TCCGGGACCTGGCGCCCAGGGGTATTCCGGCCATCGTGAATGCTGACAGCGATCACCGCGAAGCGGTCGAGGAGACCGCG
CGCAAGGCGGGCCTGCAGGTGGTGTCGTTCGGCTGGCGCGGGGAAGATCTCTGGATCGATGAGCTGATGCCCAAGGCGAC
AGGGCAGACGCTGTTTCTCTACTGGAAAGACGTTGAGCAAAAGCCCATCGAGCTGCCGCTGATCGGCGAGTTTCAGACGC
TGAACGCGCTGGCGGCGGCGGCGCTCTGCCTGTCGCTGGGCATGGAATTTCCGGCTGTGGCTGACGGGCTGGCAAACCTC
AAGCCCGTCAAGGGGCGTCTGGAGTTTGTCGGCAAGACGGAGACCGGCGCGGCCGTGTTCGTGGATTATGCCCATACGCC
CGACGGGCTGGACGTTCTGTTGCGGGCGGTGCGTCCGCATACGGCAGGACGGCTGAAGGTGATCTTCGGATGTGGCGGCG
ACCGGGACGCTAAGAAGCGGCCGATCATGGGCGAGATCGCCAGCCGGCAGGCCGACGACGTGATCGTGACCGATGACAAT
CCGCGCTCGGAAGATCCGGCAAAGATCCGCGCGTCTGTGCTGGGCGGCGCCACGGGCGCGCGCGAGATCGGCGACCGGGC
AGAGGCCATCCATGCGGTGGTCCGTGAACTGAAAAAAGGCGATACGCTAGTGATCGCCGGCAAGGGACATGAGACCGGCC
AGATCATCGGTTCGCAGGTTCTGCCCTTCTCCGATCAGGATGAGGCGCTGGCCGCCCTGAAAGGGCTTGTGCGATGA

Upstream 100 bases:

>100_bases
TTGAGCGCGTTGCGCCGTTGCTTGAGGTCGATCCGCGATTTGAGGATCTCCGGCCTGCCACAGCGCCGCCTCAGGCCCTC
TCCCAGAACAGGAGCGAACT

Downstream 100 bases:

>100_bases
ACCGCCCCCTCTGGACTTCCAGCGACATGGAGCTTGCCACCGGCGGCGACGCCAGCGCGGCGTTTGAAGTGACGGGATCG
ATTTCGATCGATACGCGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diamino-pimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKLSDLFPLAENGETEILGMTSDSRKVQPGWLFAALKGSVHDGGKFVPQAVAQGAAAILSDGGIGAVSVPHVMADEPRRA
LALAAKRFYGEQPEFVVAVTGTNGKSSTVDFARQIWSRAGIKAASMGTLGAIGPAGKVDIGHTTPDPVTVSETMKALARQ
GVTHCAMEASSHGLEQHRLDGVDLSAVAFLNLTQDHLDYHATMEAYLDAKVRLFRDLAPRGIPAIVNADSDHREAVEETA
RKAGLQVVSFGWRGEDLWIDELMPKATGQTLFLYWKDVEQKPIELPLIGEFQTLNALAAAALCLSLGMEFPAVADGLANL
KPVKGRLEFVGKTETGAAVFVDYAHTPDGLDVLLRAVRPHTAGRLKVIFGCGGDRDAKKRPIMGEIASRQADDVIVTDDN
PRSEDPAKIRASVLGGATGAREIGDRAEAIHAVVRELKKGDTLVIAGKGHETGQIIGSQVLPFSDQDEALAALKGLVR

Sequences:

>Translated_478_residues
MKLSDLFPLAENGETEILGMTSDSRKVQPGWLFAALKGSVHDGGKFVPQAVAQGAAAILSDGGIGAVSVPHVMADEPRRA
LALAAKRFYGEQPEFVVAVTGTNGKSSTVDFARQIWSRAGIKAASMGTLGAIGPAGKVDIGHTTPDPVTVSETMKALARQ
GVTHCAMEASSHGLEQHRLDGVDLSAVAFLNLTQDHLDYHATMEAYLDAKVRLFRDLAPRGIPAIVNADSDHREAVEETA
RKAGLQVVSFGWRGEDLWIDELMPKATGQTLFLYWKDVEQKPIELPLIGEFQTLNALAAAALCLSLGMEFPAVADGLANL
KPVKGRLEFVGKTETGAAVFVDYAHTPDGLDVLLRAVRPHTAGRLKVIFGCGGDRDAKKRPIMGEIASRQADDVIVTDDN
PRSEDPAKIRASVLGGATGAREIGDRAEAIHAVVRELKKGDTLVIAGKGHETGQIIGSQVLPFSDQDEALAALKGLVR
>Mature_478_residues
MKLSDLFPLAENGETEILGMTSDSRKVQPGWLFAALKGSVHDGGKFVPQAVAQGAAAILSDGGIGAVSVPHVMADEPRRA
LALAAKRFYGEQPEFVVAVTGTNGKSSTVDFARQIWSRAGIKAASMGTLGAIGPAGKVDIGHTTPDPVTVSETMKALARQ
GVTHCAMEASSHGLEQHRLDGVDLSAVAFLNLTQDHLDYHATMEAYLDAKVRLFRDLAPRGIPAIVNADSDHREAVEETA
RKAGLQVVSFGWRGEDLWIDELMPKATGQTLFLYWKDVEQKPIELPLIGEFQTLNALAAAALCLSLGMEFPAVADGLANL
KPVKGRLEFVGKTETGAAVFVDYAHTPDGLDVLLRAVRPHTAGRLKVIFGCGGDRDAKKRPIMGEIASRQADDVIVTDDN
PRSEDPAKIRASVLGGATGAREIGDRAEAIHAVVRELKKGDTLVIAGKGHETGQIIGSQVLPFSDQDEALAALKGLVR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=468, Percent_Identity=41.025641025641, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1786274, Length=331, Percent_Identity=29.9093655589124, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50808; Mature: 50808

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDLFPLAENGETEILGMTSDSRKVQPGWLFAALKGSVHDGGKFVPQAVAQGAAAILS
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHC
DGGIGAVSVPHVMADEPRRALALAAKRFYGEQPEFVVAVTGTNGKSSTVDFARQIWSRAG
CCCCCCEECCCHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
IKAASMGTLGAIGPAGKVDIGHTTPDPVTVSETMKALARQGVTHCAMEASSHGLEQHRLD
CCEECCCCCCCCCCCCCEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCC
GVDLSAVAFLNLTQDHLDYHATMEAYLDAKVRLFRDLAPRGIPAIVNADSDHREAVEETA
CCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
RKAGLQVVSFGWRGEDLWIDELMPKATGQTLFLYWKDVEQKPIELPLIGEFQTLNALAAA
HHCCCEEEEECCCCCCEEHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
ALCLSLGMEFPAVADGLANLKPVKGRLEFVGKTETGAAVFVDYAHTPDGLDVLLRAVRPH
HHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCC
TAGRLKVIFGCGGDRDAKKRPIMGEIASRQADDVIVTDDNPRSEDPAKIRASVLGGATGA
CCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCH
REIGDRAEAIHAVVRELKKGDTLVIAGKGHETGQIIGSQVLPFSDQDEALAALKGLVR
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLSDLFPLAENGETEILGMTSDSRKVQPGWLFAALKGSVHDGGKFVPQAVAQGAAAILS
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCHHHHHC
DGGIGAVSVPHVMADEPRRALALAAKRFYGEQPEFVVAVTGTNGKSSTVDFARQIWSRAG
CCCCCCEECCCHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
IKAASMGTLGAIGPAGKVDIGHTTPDPVTVSETMKALARQGVTHCAMEASSHGLEQHRLD
CCEECCCCCCCCCCCCCEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCC
GVDLSAVAFLNLTQDHLDYHATMEAYLDAKVRLFRDLAPRGIPAIVNADSDHREAVEETA
CCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
RKAGLQVVSFGWRGEDLWIDELMPKATGQTLFLYWKDVEQKPIELPLIGEFQTLNALAAA
HHCCCEEEEECCCCCCEEHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
ALCLSLGMEFPAVADGLANLKPVKGRLEFVGKTETGAAVFVDYAHTPDGLDVLLRAVRPH
HHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCC
TAGRLKVIFGCGGDRDAKKRPIMGEIASRQADDVIVTDDNPRSEDPAKIRASVLGGATGA
CCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCH
REIGDRAEAIHAVVRELKKGDTLVIAGKGHETGQIIGSQVLPFSDQDEALAALKGLVR
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]