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The map label for this gene is sir1 [H]

Identifier: 114798651

GI number: 114798651

Start: 320253

End: 321911

Strand: Direct

Name: sir1 [H]

Synonym: HNE_0325

Alternate gene names: 114798651

Gene position: 320253-321911 (Clockwise)

Preceding gene: 114798341

Following gene: 114799901

Centisome position: 8.64

GC content: 62.87

Gene sequence:

>1659_bases
ATGTATCGTTATGACCAATTCGACGCCCGCATTGTTGCCGAGCGTGTCGCCCAGTTCCGGGGGCAGGTGGCCCGCCGCCT
GTCCGGTGAGCTGCTGGAGGACGAGTTCAAGCCGCTGCGCCTGCAGAACGGCGTCTACCTCCAGCTCCACGCCTATATGC
TGCGCATCGCCATCCCCTATGGCCAGCTCAGCCCCGTGCAGCTCCGCCGCCTGGCAGAAGTCGCGCGCGACTATGACCGC
GGCTTTGGCCACTTCACCACCCGCCAGAACATCCAGTTCAACTGGGTCGCCCTGAAAGACATCCCGGACGTTCTGGAAAA
GCTCGCCGAAGTCGAGATGCACGCCATCCAGACCTCGGGCAACTGTATCCGCAATACGACCACGGATCATTTCGCCGGCG
CCGCGCAGGACGAAGTTTTCGATCCGCGTCCCTGGTGCGAGATCATCCGCCAGTGGTCCACTTTCCATCCCGAATTCGCG
TTCCTGCCGCGCAAGTTCAAGATCGCGGTCACCGCCGCCGAGCATGACCGCGCCGCCATCCGCGTGCACGATGTCGGCCT
CCACATCCGCCAGAAGGATGGCGTCACCGGTTTTGAAGTCTGGGTCGGCGGCGGGCAGGGGCGCACGCCCGTCATCGCCA
CGCTGGTCAATCCCTTCGTGCCCGAAAGCCAGATCCTCGATTATCTCGAAGCCATCATGCGCGTCTATAACCGCTATGGC
CGGCGCGATAACAAATACAAAGCCCGCATCAAGATCCTCGTCACTGAGCTCGGTGATGCCGAATACATCCGCCAGGTCGA
GGAAGAATTCGCCGCCCAGCGCGCGCATGAAAAGATCGACATGCCCGCAGACGAAATCGCGCGCATACACGCCTATTTCG
CCCCGCCGCAACTGGCTGAAAAGCCCGCCGTCTCCCGGACGCTGGAATTGGCCACCGCCGCAGACCCCGCCTTTGCCCGC
TGGGTACGGATCAATCTCCATCCTCATAAAGTGCCCGGCTACGCGTCCGTCACGGTCAGCCTGAAGCCGATCGGCATTGC
GCCGGGCGACGCCACCGACACGCAGATGGAACTCGTCGCAGACCTCGCTGAAAAGTTCGGCCATGGCGACATCCGCGTCA
GCCATGCGCAAAACCTGATCCTGCCGCATGTCGCCCTCGACGACCTGCCGGAAATCTACGCCGCCCTCCACGCCGCCGGC
CTCTCGGCCGCAAATGAGAGCCGCATCACCGACATGATCGTCTGTCCGGGCCTGGACTATTGCAACCTCGCCAACGCCCG
CTCGATCCCGGTTTCGCTGGAAGTGCAGAAACTCTTCGCCGACCCGGCATATGAGGAAGACATCGGCCGCCTGCACATCA
ACGTCTCGGGCTGCATCAATGCCTGCGGCCACCACCATGTCGGCCATATCGGCATCCTCGGCGTCGACAAGAAGGGCGAG
GAATTCTACCAAATCCTGCTCGGCGGGCGCGCGGACGAGAAAGCCGCCCTCGGCAATATCGTCGGCCCCGGCCTGAAGGC
AGAAGAGGTGCCCGGCGCCATTCACCGCGTCGTGGAATATTACCGCGCCCAGCGCACCTCAAAGGACGAAAAGTTCATCG
ACACGCTGGAACGCCTCGGCCACCAGCCCTTCCAGGAGGCGCTCTATGCCGCTGATTAA

Upstream 100 bases:

>100_bases
ATGAGGGCGGCTTCGATATTCGACTTTACCCGGCAGTCGCGCGCTGGGTTTCCCGCACTGAAGATGCGCTGGGCGTCCCC
CATGCGCAGGAGTTTGTCTG

Downstream 100 bases:

>100_bases
GAACGGCGCCGAGACCGCCAATGACTGGACCTTCGTGGCCGATGGCGCAGACTTGCCGGAAACCGGCAAGTTTTCGGTCA
CTCTGGGGCGCTTTCTCGCC

Product: putative sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MYRYDQFDARIVAERVAQFRGQVARRLSGELLEDEFKPLRLQNGVYLQLHAYMLRIAIPYGQLSPVQLRRLAEVARDYDR
GFGHFTTRQNIQFNWVALKDIPDVLEKLAEVEMHAIQTSGNCIRNTTTDHFAGAAQDEVFDPRPWCEIIRQWSTFHPEFA
FLPRKFKIAVTAAEHDRAAIRVHDVGLHIRQKDGVTGFEVWVGGGQGRTPVIATLVNPFVPESQILDYLEAIMRVYNRYG
RRDNKYKARIKILVTELGDAEYIRQVEEEFAAQRAHEKIDMPADEIARIHAYFAPPQLAEKPAVSRTLELATAADPAFAR
WVRINLHPHKVPGYASVTVSLKPIGIAPGDATDTQMELVADLAEKFGHGDIRVSHAQNLILPHVALDDLPEIYAALHAAG
LSAANESRITDMIVCPGLDYCNLANARSIPVSLEVQKLFADPAYEEDIGRLHINVSGCINACGHHHVGHIGILGVDKKGE
EFYQILLGGRADEKAALGNIVGPGLKAEEVPGAIHRVVEYYRAQRTSKDEKFIDTLERLGHQPFQEALYAAD

Sequences:

>Translated_552_residues
MYRYDQFDARIVAERVAQFRGQVARRLSGELLEDEFKPLRLQNGVYLQLHAYMLRIAIPYGQLSPVQLRRLAEVARDYDR
GFGHFTTRQNIQFNWVALKDIPDVLEKLAEVEMHAIQTSGNCIRNTTTDHFAGAAQDEVFDPRPWCEIIRQWSTFHPEFA
FLPRKFKIAVTAAEHDRAAIRVHDVGLHIRQKDGVTGFEVWVGGGQGRTPVIATLVNPFVPESQILDYLEAIMRVYNRYG
RRDNKYKARIKILVTELGDAEYIRQVEEEFAAQRAHEKIDMPADEIARIHAYFAPPQLAEKPAVSRTLELATAADPAFAR
WVRINLHPHKVPGYASVTVSLKPIGIAPGDATDTQMELVADLAEKFGHGDIRVSHAQNLILPHVALDDLPEIYAALHAAG
LSAANESRITDMIVCPGLDYCNLANARSIPVSLEVQKLFADPAYEEDIGRLHINVSGCINACGHHHVGHIGILGVDKKGE
EFYQILLGGRADEKAALGNIVGPGLKAEEVPGAIHRVVEYYRAQRTSKDEKFIDTLERLGHQPFQEALYAAD
>Mature_552_residues
MYRYDQFDARIVAERVAQFRGQVARRLSGELLEDEFKPLRLQNGVYLQLHAYMLRIAIPYGQLSPVQLRRLAEVARDYDR
GFGHFTTRQNIQFNWVALKDIPDVLEKLAEVEMHAIQTSGNCIRNTTTDHFAGAAQDEVFDPRPWCEIIRQWSTFHPEFA
FLPRKFKIAVTAAEHDRAAIRVHDVGLHIRQKDGVTGFEVWVGGGQGRTPVIATLVNPFVPESQILDYLEAIMRVYNRYG
RRDNKYKARIKILVTELGDAEYIRQVEEEFAAQRAHEKIDMPADEIARIHAYFAPPQLAEKPAVSRTLELATAADPAFAR
WVRINLHPHKVPGYASVTVSLKPIGIAPGDATDTQMELVADLAEKFGHGDIRVSHAQNLILPHVALDDLPEIYAALHAAG
LSAANESRITDMIVCPGLDYCNLANARSIPVSLEVQKLFADPAYEEDIGRLHINVSGCINACGHHHVGHIGILGVDKKGE
EFYQILLGGRADEKAALGNIVGPGLKAEEVPGAIHRVVEYYRAQRTSKDEKFIDTLERLGHQPFQEALYAAD

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 61877; Mature: 61877

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYDQFDARIVAERVAQFRGQVARRLSGELLEDEFKPLRLQNGVYLQLHAYMLRIAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEEEHHHCCCC
GQLSPVQLRRLAEVARDYDRGFGHFTTRQNIQFNWVALKDIPDVLEKLAEVEMHAIQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
NCIRNTTTDHFAGAAQDEVFDPRPWCEIIRQWSTFHPEFAFLPRKFKIAVTAAEHDRAAI
CEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCEEEEEEEECCCCCEEE
RVHDVGLHIRQKDGVTGFEVWVGGGQGRTPVIATLVNPFVPESQILDYLEAIMRVYNRYG
EEEECCEEEEECCCCCEEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
RRDNKYKARIKILVTELGDAEYIRQVEEEFAAQRAHEKIDMPADEIARIHAYFAPPQLAE
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHC
KPAVSRTLELATAADPAFARWVRINLHPHKVPGYASVTVSLKPIGIAPGDATDTQMELVA
CCHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHH
DLAEKFGHGDIRVSHAQNLILPHVALDDLPEIYAALHAAGLSAANESRITDMIVCPGLDY
HHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
CNLANARSIPVSLEVQKLFADPAYEEDIGRLHINVSGCINACGHHHVGHIGILGVDKKGE
CCCCCCCCCCEEEEHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCCCCCEEEEEECCCCHH
EFYQILLGGRADEKAALGNIVGPGLKAEEVPGAIHRVVEYYRAQRTSKDEKFIDTLERLG
HHHHHHHCCCCCCHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
HQPFQEALYAAD
CCCHHHHHHCCC
>Mature Secondary Structure
MYRYDQFDARIVAERVAQFRGQVARRLSGELLEDEFKPLRLQNGVYLQLHAYMLRIAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEEEHHHCCCC
GQLSPVQLRRLAEVARDYDRGFGHFTTRQNIQFNWVALKDIPDVLEKLAEVEMHAIQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
NCIRNTTTDHFAGAAQDEVFDPRPWCEIIRQWSTFHPEFAFLPRKFKIAVTAAEHDRAAI
CEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCEEEEEEEECCCCCEEE
RVHDVGLHIRQKDGVTGFEVWVGGGQGRTPVIATLVNPFVPESQILDYLEAIMRVYNRYG
EEEECCEEEEECCCCCEEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
RRDNKYKARIKILVTELGDAEYIRQVEEEFAAQRAHEKIDMPADEIARIHAYFAPPQLAE
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHC
KPAVSRTLELATAADPAFARWVRINLHPHKVPGYASVTVSLKPIGIAPGDATDTQMELVA
CCHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHH
DLAEKFGHGDIRVSHAQNLILPHVALDDLPEIYAALHAAGLSAANESRITDMIVCPGLDY
HHHHHCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
CNLANARSIPVSLEVQKLFADPAYEEDIGRLHINVSGCINACGHHHVGHIGILGVDKKGE
CCCCCCCCCCEEEEHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCCCCCEEEEEECCCCHH
EFYQILLGGRADEKAALGNIVGPGLKAEEVPGAIHRVVEYYRAQRTSKDEKFIDTLERLG
HHHHHHHCCCCCCHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
HQPFQEALYAAD
CCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA