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The map label for this gene is hemK [H]

Identifier: 114798227

GI number: 114798227

Start: 629469

End: 630326

Strand: Direct

Name: hemK [H]

Synonym: HNE_0629

Alternate gene names: 114798227

Gene position: 629469-630326 (Clockwise)

Preceding gene: 114799445

Following gene: 114797461

Centisome position: 16.99

GC content: 67.13

Gene sequence:

>858_bases
GTGACGCCGCCCACCTATGATGATCTGATCCGGCATGCGGCGCGCCGTTTCAAGGACGCCGGTCTTTCGGAGGCGTATCA
GAATGCCGTGCTGCTGATGGTTCATGCGTTCGGCGACACGCGTGCGGTGCTGATCTCGGCGGGCAATGCGCCTGTGCCAA
AGGCTGTGGAAGACCTCTATCTCGCTGCCGTCGAGCGCCGCGCCGCGCGTGAGCCGATCCAGCACATCCTCGGCGTGACG
CAATTCTACGGCCTCGACATCCGCACAGACGCGCGCGCGCTGATCCCGCGCATCGACAGCGAGTGCGTGGTCGAGGCGGC
GCTGGACCGGATGCCGAAGAACACCCCGCTCGTCCTTGCGGATCTGGGCACGGGTTCGGGCTGCCTCCTCGCCGCGCTCC
TGTCCCAGCGCCCGCTCGCCCGCGGGGAAGGGGTGGAGGCCAGCGCCGAGGCGGCGAGCCTCGCCCGCGAGAATCTCGAC
GCCCTGAACCTTGCGGCGCGCGGCGCGGTCTTTGATGGCAGCTGGACCCAGTGGCAGGGCTGGGAAAACGCAGACCTCAT
AATCTCCAATCCACCCTACATTGCATCGGCCGAGATTGCGGTGCTGGAGCCTGAAGTGCGCGCGCATGACCCCCTGAGCG
CGCTCGATGGCGGGGCGGACGGGCTGGACGCTTACCGCGAGATCATCGCGCTCGCTGCCGCCCGGATGAAACCCGGCGCC
TGGCTGGTGTTCGAGATCGGGCACGACCAGAAAGCGGCTCTGGAGTGGTTAATGGCACAGACCGGCTTTGAGGCCATTGC
CTCTGGTCAGGATCTGGGCGGCAATGACCGCTGGGTTGGCGCCCGCAGACCCTGTTGA

Upstream 100 bases:

>100_bases
CCGGTGACGGGGCAGACGCAAATATCGGCGTATCCGTGTTGCGTGATTTCACACTGGTGATCGATTTCGCAGGAAACCGT
GTGTGGCTGGAACCGCGCGC

Downstream 100 bases:

>100_bases
GCCCGCTTACATTTTCTCTTGGCGGGCCGGTCGATCCACGTTACACAAGAGGCCGGTGAGGGGCCGCCCGCAATTTGCGC
GGGTGCAGATGCAAGCTCCC

Product: HemK family methyltransferase

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTPPTYDDLIRHAARRFKDAGLSEAYQNAVLLMVHAFGDTRAVLISAGNAPVPKAVEDLYLAAVERRAAREPIQHILGVT
QFYGLDIRTDARALIPRIDSECVVEAALDRMPKNTPLVLADLGTGSGCLLAALLSQRPLARGEGVEASAEAASLARENLD
ALNLAARGAVFDGSWTQWQGWENADLIISNPPYIASAEIAVLEPEVRAHDPLSALDGGADGLDAYREIIALAAARMKPGA
WLVFEIGHDQKAALEWLMAQTGFEAIASGQDLGGNDRWVGARRPC

Sequences:

>Translated_285_residues
MTPPTYDDLIRHAARRFKDAGLSEAYQNAVLLMVHAFGDTRAVLISAGNAPVPKAVEDLYLAAVERRAAREPIQHILGVT
QFYGLDIRTDARALIPRIDSECVVEAALDRMPKNTPLVLADLGTGSGCLLAALLSQRPLARGEGVEASAEAASLARENLD
ALNLAARGAVFDGSWTQWQGWENADLIISNPPYIASAEIAVLEPEVRAHDPLSALDGGADGLDAYREIIALAAARMKPGA
WLVFEIGHDQKAALEWLMAQTGFEAIASGQDLGGNDRWVGARRPC
>Mature_284_residues
TPPTYDDLIRHAARRFKDAGLSEAYQNAVLLMVHAFGDTRAVLISAGNAPVPKAVEDLYLAAVERRAAREPIQHILGVTQ
FYGLDIRTDARALIPRIDSECVVEAALDRMPKNTPLVLADLGTGSGCLLAALLSQRPLARGEGVEASAEAASLARENLDA
LNLAARGAVFDGSWTQWQGWENADLIISNPPYIASAEIAVLEPEVRAHDPLSALDGGADGLDAYREIIALAAARMKPGAW
LVFEIGHDQKAALEWLMAQTGFEAIASGQDLGGNDRWVGARRPC

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=295, Percent_Identity=29.4915254237288, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1787463, Length=252, Percent_Identity=31.3492063492063, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI87082085, Length=199, Percent_Identity=27.6381909547739, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582226, Length=225, Percent_Identity=32.4444444444444, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30557; Mature: 30426

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPTYDDLIRHAARRFKDAGLSEAYQNAVLLMVHAFGDTRAVLISAGNAPVPKAVEDLY
CCCCCHHHHHHHHHHHHHHCCHHHHHHCEEEEEEEECCCCEEEEEECCCCCCHHHHHHHH
LAAVERRAAREPIQHILGVTQFYGLDIRTDARALIPRIDSECVVEAALDRMPKNTPLVLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCEEEE
DLGTGSGCLLAALLSQRPLARGEGVEASAEAASLARENLDALNLAARGAVFDGSWTQWQG
ECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCCC
WENADLIISNPPYIASAEIAVLEPEVRAHDPLSALDGGADGLDAYREIIALAAARMKPGA
CCCCCEEECCCCCEECCEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
WLVFEIGHDQKAALEWLMAQTGFEAIASGQDLGGNDRWVGARRPC
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPPTYDDLIRHAARRFKDAGLSEAYQNAVLLMVHAFGDTRAVLISAGNAPVPKAVEDLY
CCCCHHHHHHHHHHHHHHCCHHHHHHCEEEEEEEECCCCEEEEEECCCCCCHHHHHHHH
LAAVERRAAREPIQHILGVTQFYGLDIRTDARALIPRIDSECVVEAALDRMPKNTPLVLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCEEEE
DLGTGSGCLLAALLSQRPLARGEGVEASAEAASLARENLDALNLAARGAVFDGSWTQWQG
ECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCCC
WENADLIISNPPYIASAEIAVLEPEVRAHDPLSALDGGADGLDAYREIIALAAARMKPGA
CCCCCEEECCCCCEECCEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
WLVFEIGHDQKAALEWLMAQTGFEAIASGQDLGGNDRWVGARRPC
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]