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The map label for this gene is prfA

Identifier: 114797902

GI number: 114797902

Start: 626715

End: 627773

Strand: Direct

Name: prfA

Synonym: HNE_0626

Alternate gene names: 114797902

Gene position: 626715-627773 (Clockwise)

Preceding gene: 114798918

Following gene: 114800266

Centisome position: 16.92

GC content: 63.08

Gene sequence:

>1059_bases
TTGCGCCTTCAGCAGGTGATCGATCGGTATGAGGAGGTTGAGGCCCGGATGGGCGCGACTTCCAACACTAACGAGATCAT
TGCCCTTTCAAAAGAACACGCCGAGCTCCGCCCGGTGGTCGAGAAGGCGCGCGCGCTGATGACCGTCCGCAAGGGGCTCT
CCGAGGCCGAGGCGATGATCGCCTCAGGCGACAGGGAAATGGCCGAACTTGCAGAAATGGAGATCGACGACCTGCGCGAG
CGTCTTCCCGGTCTGGAGCAGGAAATGCAGCTTCTGCTGCTGCCCAAGGATGTCGATGACAAGGCCGATATCGTGCTTGA
AGTGCGTGCCGGTACAGGCGGCGATGAAGCGGCCCTGTTCGCGGGCGATCTCTTCCGGATGTACACCCGCTTTGCGCAAA
TCATGGGCTGGAGCGTCGAGATCGTCGATGTGGCGCCTGGCGACGCCGGCGGCTACAAGGAAATCATTGCTAACGTGTCG
GGCGACGGCGTGTTCGGGCGGATGAAGTGGGAGAGCGGCGTGCACCGCGTCCAGCGCGTGCCGGCTACCGAGACGCAGGG
ACGTATTCATACATCGGCGGCGACGGTTGCCGTCCTTCCGGCGCCGGAGAATATCGAGATCGAGGTGCGCGCCGACGATA
TCCGTATCGACACGATGCGCTCGTCCGGAGCCGGCGGCCAGCACGTCAACACGACGGACTCGGCTGTGCGCATCACCCAC
TTGCCGACCGGCATCATGGTGACGAGTTCGGAGAAGTCCCAGCACGTCAACCGCGAGAAGGCGATGGATCAGCTGAAAAT
CCGCCTCTATGAGCGCGAGCGCGCCGAGAAGGATGCCGCCCGCTCCGAAGCGCGCGCCAGCCAGATCGGCTCGGGCGACC
GCAGCCAGAAGGTGCGCACCTATAACTATCCGGAAAACCGCCTGACCGATCACCGTATCGGGCTGACGCTCTACTCGCTC
GACCGGATCATCACGGGCGACAAGCTGGGCGATGTGATCGAAGCTCTGATTACGGAAGATCAGTCCCGGCGCCTCGCCTC
CCTGGAGGCAGGTGCATGA

Upstream 100 bases:

>100_bases
GGCAGGCAAGGAAGCCCTGCGCATCTTCGGCTGACATCAAGGCGGGCTTCCATGAAGCCTGAAAGCGGATTAAAGGGCGG
GCATGTCAGCCTTGTCCCAA

Downstream 100 bases:

>100_bases
GCGGCGGGCTTGCCGGCCGGGTGACAAGCCTCTGGCGCCATCCGGTGAAGGGGTTTACGCCCGAACGCCTGAGCGAAGTG
ACCCTCACGGCCGGCGGGCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRLQQVIDRYEEVEARMGATSNTNEIIALSKEHAELRPVVEKARALMTVRKGLSEAEAMIASGDREMAELAEMEIDDLRE
RLPGLEQEMQLLLLPKDVDDKADIVLEVRAGTGGDEAALFAGDLFRMYTRFAQIMGWSVEIVDVAPGDAGGYKEIIANVS
GDGVFGRMKWESGVHRVQRVPATETQGRIHTSAATVAVLPAPENIEIEVRADDIRIDTMRSSGAGGQHVNTTDSAVRITH
LPTGIMVTSSEKSQHVNREKAMDQLKIRLYERERAEKDAARSEARASQIGSGDRSQKVRTYNYPENRLTDHRIGLTLYSL
DRIITGDKLGDVIEALITEDQSRRLASLEAGA

Sequences:

>Translated_352_residues
MRLQQVIDRYEEVEARMGATSNTNEIIALSKEHAELRPVVEKARALMTVRKGLSEAEAMIASGDREMAELAEMEIDDLRE
RLPGLEQEMQLLLLPKDVDDKADIVLEVRAGTGGDEAALFAGDLFRMYTRFAQIMGWSVEIVDVAPGDAGGYKEIIANVS
GDGVFGRMKWESGVHRVQRVPATETQGRIHTSAATVAVLPAPENIEIEVRADDIRIDTMRSSGAGGQHVNTTDSAVRITH
LPTGIMVTSSEKSQHVNREKAMDQLKIRLYERERAEKDAARSEARASQIGSGDRSQKVRTYNYPENRLTDHRIGLTLYSL
DRIITGDKLGDVIEALITEDQSRRLASLEAGA
>Mature_352_residues
MRLQQVIDRYEEVEARMGATSNTNEIIALSKEHAELRPVVEKARALMTVRKGLSEAEAMIASGDREMAELAEMEIDDLRE
RLPGLEQEMQLLLLPKDVDDKADIVLEVRAGTGGDEAALFAGDLFRMYTRFAQIMGWSVEIVDVAPGDAGGYKEIIANVS
GDGVFGRMKWESGVHRVQRVPATETQGRIHTSAATVAVLPAPENIEIEVRADDIRIDTMRSSGAGGQHVNTTDSAVRITH
LPTGIMVTSSEKSQHVNREKAMDQLKIRLYERERAEKDAARSEARASQIGSGDRSQKVRTYNYPENRLTDHRIGLTLYSL
DRIITGDKLGDVIEALITEDQSRRLASLEAGA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=280, Percent_Identity=47.1428571428571, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI34577120, Length=322, Percent_Identity=37.888198757764, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI166795305, Length=191, Percent_Identity=47.6439790575916, Blast_Score=191, Evalue=7e-49,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=53.125, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=37.4631268436578, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=277, Percent_Identity=37.5451263537906, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=41.1949685534591, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI19921226, Length=356, Percent_Identity=40.4494382022472, Blast_Score=237, Evalue=7e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_HYPNA (Q0C4I8)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759355.1
- ProteinModelPortal:   Q0C4I8
- SMR:   Q0C4I8
- STRING:   Q0C4I8
- GeneID:   4288090
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0626
- NMPDR:   fig|228405.5.peg.614
- TIGR:   HNE_0626
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   HNEP81032:HNE_0626-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38911; Mature: 38911

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQQVIDRYEEVEARMGATSNTNEIIALSKEHAELRPVVEKARALMTVRKGLSEAEAMI
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDREMAELAEMEIDDLRERLPGLEQEMQLLLLPKDVDDKADIVLEVRAGTGGDEAALF
HCCCHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHH
AGDLFRMYTRFAQIMGWSVEIVDVAPGDAGGYKEIIANVSGDGVFGRMKWESGVHRVQRV
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHCC
PATETQGRIHTSAATVAVLPAPENIEIEVRADDIRIDTMRSSGAGGQHVNTTDSAVRITH
CCCCCCCEEEECCEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEEEE
LPTGIMVTSSEKSQHVNREKAMDQLKIRLYERERAEKDAARSEARASQIGSGDRSQKVRT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
YNYPENRLTDHRIGLTLYSLDRIITGDKLGDVIEALITEDQSRRLASLEAGA
ECCCCHHCCCHHHCEEEEHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCC
>Mature Secondary Structure
MRLQQVIDRYEEVEARMGATSNTNEIIALSKEHAELRPVVEKARALMTVRKGLSEAEAMI
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDREMAELAEMEIDDLRERLPGLEQEMQLLLLPKDVDDKADIVLEVRAGTGGDEAALF
HCCCHHHHHHHHCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHH
AGDLFRMYTRFAQIMGWSVEIVDVAPGDAGGYKEIIANVSGDGVFGRMKWESGVHRVQRV
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHCC
PATETQGRIHTSAATVAVLPAPENIEIEVRADDIRIDTMRSSGAGGQHVNTTDSAVRITH
CCCCCCCEEEECCEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEEEE
LPTGIMVTSSEKSQHVNREKAMDQLKIRLYERERAEKDAARSEARASQIGSGDRSQKVRT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
YNYPENRLTDHRIGLTLYSLDRIITGDKLGDVIEALITEDQSRRLASLEAGA
ECCCCHHCCCHHHCEEEEHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA