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The map label for this gene is ispG

Identifier: 114797345

GI number: 114797345

Start: 622497

End: 623639

Strand: Direct

Name: ispG

Synonym: HNE_0621

Alternate gene names: 114797345

Gene position: 622497-623639 (Clockwise)

Preceding gene: 114799427

Following gene: 114799691

Centisome position: 16.8

GC content: 64.22

Gene sequence:

>1143_bases
ATGAGCCACAATCCGATCCGTCCCTGGCGCAATATCGAGCGCCGCAAGTCCCGCCAGGTGCGGGTGGGGAATCTGATGGT
CGGGGGCGACGCGCCGATTGCCGTCCAGACGATGACCAACACGCCCACCGAGGACGCCGCCGCCACGATCGCCCAGATCC
TGCGCTGCGCGGAGGCGGGCGCCGACATTGTCCGCGTGTCCTGCCCGACCGAAGAGTCGACCAAAGCCATGCGCGAGATC
GTCAAGGCGAGCCCGGTGCCGCTCGTGGCCGACATCCACTTTCACTACAAACGCGGCATTGAAGCGGCCGACGCGGGCGC
TGCCTGCCTGCGCATCAATCCCGGCAATATCGGCTCCCATGCCCGTGTGAAGGAAGTTGTCGCCGCCGCGCGCGCCAATG
GCTGCTCGATGCGCATTGGTGTCAATGGCGGCAGCCTGGAGCGCCACCTTCTGGAAAAATATGGCGAGCCATGCCCGGAC
GCGATGGTCGAAAGCGCGCTGGACCATGCCCGCATTCTCGATGATCTGGGCTTTTACGACTACAAGATCTCGGTCAAGGC
GTCCGACATGTTCCTCACCGTGGCGGCCTATCACGCCCTCGCCGAGGCAACCGACGCGCCGCTGCATCTGGGCATTACAG
AAGCGGGCGGCCTGCGTACCGGTACGGTCAAATCCTCCATCGGCATGGGCGCGCTGCTCTGGGCCGGCATCGGCGACACC
ATCCGCGTCTCTCTCTCGGCAGAGCCCGAAGAAGAGGTGAAGGTCGGCTTCGAGATGCTCAAATCCCTCGGCCTGCGCAC
GCGCGGCGTGAACATCGTCGCGTGCCCCAGCTGCGCGCGTCAGGGCTTTGACGTGATCCGCACCGTCGAGACACTGGAAA
AGCGCCTCGCGCATATCTCCGAGCCGATTTCGCTGTCGATCATCGGCTGCGTCGTGAACGGTCCGGGCGAAGCCTCCCTT
ACCGATCTCGGTTTCACCGGCGGCGGCAAGGAAAGCGGCAAGATGTTCGTCAACGGACGGGCCGACCACAATGTCGCCAA
TGCCGATATGGTCGAACACATCGTCAAGCTGGTCGAGGACAAGGCCGCCCGTTTGAAAGCTCAGCGCGACGCAGAAGAAG
CCACCGAAGTTCCGGCAGAGTAG

Upstream 100 bases:

>100_bases
AACCAGCGACACCCTGAGGCCTTTACGGAGCGCCTCACACACGCCACATCTGGCCTGAACTCCTCTGCGCCTGCAGGGAA
GCTAGTCTGCGAGACGTCCG

Downstream 100 bases:

>100_bases
CGCGTTACATCAAAGCGGCGCGCAGCGGCTCGCCGGGATCAGCGCGGCAGCAGCTTCATACGCGGCCTGCGACGGCGCCA
GCCGGCGGATCAGTGCCAGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSHNPIRPWRNIERRKSRQVRVGNLMVGGDAPIAVQTMTNTPTEDAAATIAQILRCAEAGADIVRVSCPTEESTKAMREI
VKASPVPLVADIHFHYKRGIEAADAGAACLRINPGNIGSHARVKEVVAAARANGCSMRIGVNGGSLERHLLEKYGEPCPD
AMVESALDHARILDDLGFYDYKISVKASDMFLTVAAYHALAEATDAPLHLGITEAGGLRTGTVKSSIGMGALLWAGIGDT
IRVSLSAEPEEEVKVGFEMLKSLGLRTRGVNIVACPSCARQGFDVIRTVETLEKRLAHISEPISLSIIGCVVNGPGEASL
TDLGFTGGGKESGKMFVNGRADHNVANADMVEHIVKLVEDKAARLKAQRDAEEATEVPAE

Sequences:

>Translated_380_residues
MSHNPIRPWRNIERRKSRQVRVGNLMVGGDAPIAVQTMTNTPTEDAAATIAQILRCAEAGADIVRVSCPTEESTKAMREI
VKASPVPLVADIHFHYKRGIEAADAGAACLRINPGNIGSHARVKEVVAAARANGCSMRIGVNGGSLERHLLEKYGEPCPD
AMVESALDHARILDDLGFYDYKISVKASDMFLTVAAYHALAEATDAPLHLGITEAGGLRTGTVKSSIGMGALLWAGIGDT
IRVSLSAEPEEEVKVGFEMLKSLGLRTRGVNIVACPSCARQGFDVIRTVETLEKRLAHISEPISLSIIGCVVNGPGEASL
TDLGFTGGGKESGKMFVNGRADHNVANADMVEHIVKLVEDKAARLKAQRDAEEATEVPAE
>Mature_379_residues
SHNPIRPWRNIERRKSRQVRVGNLMVGGDAPIAVQTMTNTPTEDAAATIAQILRCAEAGADIVRVSCPTEESTKAMREIV
KASPVPLVADIHFHYKRGIEAADAGAACLRINPGNIGSHARVKEVVAAARANGCSMRIGVNGGSLERHLLEKYGEPCPDA
MVESALDHARILDDLGFYDYKISVKASDMFLTVAAYHALAEATDAPLHLGITEAGGLRTGTVKSSIGMGALLWAGIGDTI
RVSLSAEPEEEVKVGFEMLKSLGLRTRGVNIVACPSCARQGFDVIRTVETLEKRLAHISEPISLSIIGCVVNGPGEASLT
DLGFTGGGKESGKMFVNGRADHNVANADMVEHIVKLVEDKAARLKAQRDAEEATEVPAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=358, Percent_Identity=55.0279329608939, Blast_Score=396, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_HYPNA (Q0C4J3)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759350.1
- ProteinModelPortal:   Q0C4J3
- STRING:   Q0C4J3
- GeneID:   4287199
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0621
- NMPDR:   fig|228405.5.peg.609
- TIGR:   HNE_0621
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q0C4J3
- ProtClustDB:   PRK00366
- BioCyc:   HNEP81032:HNE_0621-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 40478; Mature: 40347

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNPIRPWRNIERRKSRQVRVGNLMVGGDAPIAVQTMTNTPTEDAAATIAQILRCAEAG
CCCCCCCCHHHHHHHHCCCEEECEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
ADIVRVSCPTEESTKAMREIVKASPVPLVADIHFHYKRGIEAADAGAACLRINPGNIGSH
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCEEEEECCCCCCCH
ARVKEVVAAARANGCSMRIGVNGGSLERHLLEKYGEPCPDAMVESALDHARILDDLGFYD
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEE
YKISVKASDMFLTVAAYHALAEATDAPLHLGITEAGGLRTGTVKSSIGMGALLWAGIGDT
EEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCHHHHHHCCCCE
IRVSLSAEPEEEVKVGFEMLKSLGLRTRGVNIVACPSCARQGFDVIRTVETLEKRLAHIS
EEEEECCCCHHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
EPISLSIIGCVVNGPGEASLTDLGFTGGGKESGKMFVNGRADHNVANADMVEHIVKLVED
CCCEEEEEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
KAARLKAQRDAEEATEVPAE
HHHHHHHHHCHHHHHCCCCC
>Mature Secondary Structure 
SHNPIRPWRNIERRKSRQVRVGNLMVGGDAPIAVQTMTNTPTEDAAATIAQILRCAEAG
CCCCCCCHHHHHHHHCCCEEECEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
ADIVRVSCPTEESTKAMREIVKASPVPLVADIHFHYKRGIEAADAGAACLRINPGNIGSH
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCEEEEECCCCCCCH
ARVKEVVAAARANGCSMRIGVNGGSLERHLLEKYGEPCPDAMVESALDHARILDDLGFYD
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEE
YKISVKASDMFLTVAAYHALAEATDAPLHLGITEAGGLRTGTVKSSIGMGALLWAGIGDT
EEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCHHHHHHCCCCE
IRVSLSAEPEEEVKVGFEMLKSLGLRTRGVNIVACPSCARQGFDVIRTVETLEKRLAHIS
EEEEECCCCHHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
EPISLSIIGCVVNGPGEASLTDLGFTGGGKESGKMFVNGRADHNVANADMVEHIVKLVED
CCCEEEEEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
KAARLKAQRDAEEATEVPAE
HHHHHHHHHCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA