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The map label for this gene is nusA [H]

Identifier: 114331633

GI number: 114331633

Start: 1731647

End: 1733119

Strand: Reverse

Name: nusA [H]

Synonym: Neut_1647

Alternate gene names: 114331633

Gene position: 1733119-1731647 (Counterclockwise)

Preceding gene: 114331634

Following gene: 114331632

Centisome position: 65.13

GC content: 47.52

Gene sequence:

>1473_bases
ATGAGCCGCGAGATTTTACTGTTGGTCAATGCTTTGGCGCATGAGAAGAATGTGGATAAAAACATTGTTTTTACCGCACT
CGAAATGGCTCTGGCTTCTGCTGCCAAGAAGCATGCCCGGGATGATATTGATGTACGTGTCTCTATCGACCGAGATACGG
GTGAATTTCAATGCTTTCGCAGATGGTTGATTGTTGCCGATGATGTAGTGGAGCATCCTGAACGGCAAATTGCACTGGTT
GAGGCTGTAAAGCAGAATCCGCAATCCGTTCCGGGTGAATATACGGAAGAGCTAATGGAGGATGTTACATTTGACCGGAT
CGGGGCACAGGCCGCGAAGCAGGTTATCTTTCAGAAAATACGAGATGCCGAACGCGAACAGAATTTGAATGATTTTCTTG
AGCGCGGGGAGTATCTAGTAAGTGGTGTGATCAAGCGTATGGATCGCGGGAATGCGATCATTGAATTCGGCAAGATCGAA
GCAGTTTTGCCGCGTGATCAAATGATCTCGCATGAGAATCTGAGAATGGGGGATCGCGTTCGCGCTTATCTGCTCAAAAT
TGATCGTGCTGTCAGAGGACCACAACTGGTGTTATCCAGAACTTCAGCAGATTTTCTGGTCAAATTGTTCGAACTTGAGG
TGCCGGAGATAGAGGAGGGTATTCTGGAAATCAGGGCGGCAGCTAGAGATCCGGGTTCCAGGGCAAAAATCGCGGTTAAA
TCAAATGATGCGCGTGTTGACCCAATTGGTACTTGTGTTGGCATGCGTGGTTCTAGGGTACAGGCGGTTACCGGTGAGCT
GGCGGGTGAGCGGGTGGATATTATTCAATGGTCAGATGATCAGGCTACATTTGTCGTGAATGCGCTGGCGCCTGCTGTGA
TTAGCAGTATAGTAATGGATGAAGATAAGCATAGTATGGATGTTGTAGTGGATGAGGAAAATCTGGCTCAGGCTATCGGG
CGAGGCGGGCAGAATGTTCGGCTGGCATCAGAGTTGACGGGCTGGCAGCTCAATATCATGACAGTGGAAGAATCTCACGA
AAAGAGTGAGGAGGAAGCTGCTTCCATCTGTGAATTATTTATGGAAAAGCTTGATGTGGATGAAGAGGTCGGGCGTATCC
TGGTAGATGAGGGTTTTTCTACACTGGAAGAAATTGCGTATATTCCAATTGAAGAAATGCTGGAAATCCAGGAATTTGAT
CATGAGACAATTGAAGAGTTACGTAATCGTGCCCGCAATGTTTTGTTGACGGCTGCGATTGTAACAGAAGAAAAAATCGG
ACATGCGTCTGAAGCATTACTTGCGTTGGAAGGAATGGATATTGATCTTGTGCGTGAGTTGGCTGCTAAAGGGATCAGCA
CTCAGGAAGAGCTTGCTGATCTGGCGGCGGATGATCTGGTCGAGTTGACGGGTGTGGATCTTGATCGGGCAAGCAAAATT
ATTATTAAGGCACGCGAACCCTGGTTTACTTGA

Upstream 100 bases:

>100_bases
TTTGAATTGAAAAATCTGGAAAAGGCCCGGCTGATTCCAAAATTGTAATTCGGGTAAATCTGTCGATGGATGTAAGGTTG
AAAAATAGGGCTGGAGGAAT

Downstream 100 bases:

>100_bases
ATTTCGATATAAAGCGGACAAATCCCGTATCTGTTTCCGGAGCGGGATATGAGTTGGTTAAGGAAATATTGATTTTGTTT
TTTATGATTTTTGTTGGATT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSREILLLVNALAHEKNVDKNIVFTALEMALASAAKKHARDDIDVRVSIDRDTGEFQCFRRWLIVADDVVEHPERQIALV
EAVKQNPQSVPGEYTEELMEDVTFDRIGAQAAKQVIFQKIRDAEREQNLNDFLERGEYLVSGVIKRMDRGNAIIEFGKIE
AVLPRDQMISHENLRMGDRVRAYLLKIDRAVRGPQLVLSRTSADFLVKLFELEVPEIEEGILEIRAAARDPGSRAKIAVK
SNDARVDPIGTCVGMRGSRVQAVTGELAGERVDIIQWSDDQATFVVNALAPAVISSIVMDEDKHSMDVVVDEENLAQAIG
RGGQNVRLASELTGWQLNIMTVEESHEKSEEEAASICELFMEKLDVDEEVGRILVDEGFSTLEEIAYIPIEEMLEIQEFD
HETIEELRNRARNVLLTAAIVTEEKIGHASEALLALEGMDIDLVRELAAKGISTQEELADLAADDLVELTGVDLDRASKI
IIKAREPWFT

Sequences:

>Translated_490_residues
MSREILLLVNALAHEKNVDKNIVFTALEMALASAAKKHARDDIDVRVSIDRDTGEFQCFRRWLIVADDVVEHPERQIALV
EAVKQNPQSVPGEYTEELMEDVTFDRIGAQAAKQVIFQKIRDAEREQNLNDFLERGEYLVSGVIKRMDRGNAIIEFGKIE
AVLPRDQMISHENLRMGDRVRAYLLKIDRAVRGPQLVLSRTSADFLVKLFELEVPEIEEGILEIRAAARDPGSRAKIAVK
SNDARVDPIGTCVGMRGSRVQAVTGELAGERVDIIQWSDDQATFVVNALAPAVISSIVMDEDKHSMDVVVDEENLAQAIG
RGGQNVRLASELTGWQLNIMTVEESHEKSEEEAASICELFMEKLDVDEEVGRILVDEGFSTLEEIAYIPIEEMLEIQEFD
HETIEELRNRARNVLLTAAIVTEEKIGHASEALLALEGMDIDLVRELAAKGISTQEELADLAADDLVELTGVDLDRASKI
IIKAREPWFT
>Mature_489_residues
SREILLLVNALAHEKNVDKNIVFTALEMALASAAKKHARDDIDVRVSIDRDTGEFQCFRRWLIVADDVVEHPERQIALVE
AVKQNPQSVPGEYTEELMEDVTFDRIGAQAAKQVIFQKIRDAEREQNLNDFLERGEYLVSGVIKRMDRGNAIIEFGKIEA
VLPRDQMISHENLRMGDRVRAYLLKIDRAVRGPQLVLSRTSADFLVKLFELEVPEIEEGILEIRAAARDPGSRAKIAVKS
NDARVDPIGTCVGMRGSRVQAVTGELAGERVDIIQWSDDQATFVVNALAPAVISSIVMDEDKHSMDVVVDEENLAQAIGR
GGQNVRLASELTGWQLNIMTVEESHEKSEEEAASICELFMEKLDVDEEVGRILVDEGFSTLEEIAYIPIEEMLEIQEFDH
ETIEELRNRARNVLLTAAIVTEEKIGHASEALLALEGMDIDLVRELAAKGISTQEELADLAADDLVELTGVDLDRASKII
IKAREPWFT

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=51.0121457489879, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54776; Mature: 54645

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREILLLVNALAHEKNVDKNIVFTALEMALASAAKKHARDDIDVRVSIDRDTGEFQCFR
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
RWLIVADDVVEHPERQIALVEAVKQNPQSVPGEYTEELMEDVTFDRIGAQAAKQVIFQKI
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAEREQNLNDFLERGEYLVSGVIKRMDRGNAIIEFGKIEAVLPRDQMISHENLRMGDRV
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHCCCCCCCCHHHH
RAYLLKIDRAVRGPQLVLSRTSADFLVKLFELEVPEIEEGILEIRAAARDPGSRAKIAVK
HHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
SNDARVDPIGTCVGMRGSRVQAVTGELAGERVDIIQWSDDQATFVVNALAPAVISSIVMD
CCCCCCCCHHHHHCCCCCEEHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
EDKHSMDVVVDEENLAQAIGRGGQNVRLASELTGWQLNIMTVEESHEKSEEEAASICELF
CCCCCCEEEECHHHHHHHHCCCCCCEEEHHHCCCCEEEEEEECCHHCCCHHHHHHHHHHH
MEKLDVDEEVGRILVDEGFSTLEEIAYIPIEEMLEIQEFDHETIEELRNRARNVLLTAAI
HHHCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VTEEKIGHASEALLALEGMDIDLVRELAAKGISTQEELADLAADDLVELTGVDLDRASKI
HHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHCCCEE
IIKAREPWFT
EEEECCCCCC
>Mature Secondary Structure 
SREILLLVNALAHEKNVDKNIVFTALEMALASAAKKHARDDIDVRVSIDRDTGEFQCFR
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
RWLIVADDVVEHPERQIALVEAVKQNPQSVPGEYTEELMEDVTFDRIGAQAAKQVIFQKI
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAEREQNLNDFLERGEYLVSGVIKRMDRGNAIIEFGKIEAVLPRDQMISHENLRMGDRV
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHCCCCCCCCHHHH
RAYLLKIDRAVRGPQLVLSRTSADFLVKLFELEVPEIEEGILEIRAAARDPGSRAKIAVK
HHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
SNDARVDPIGTCVGMRGSRVQAVTGELAGERVDIIQWSDDQATFVVNALAPAVISSIVMD
CCCCCCCCHHHHHCCCCCEEHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
EDKHSMDVVVDEENLAQAIGRGGQNVRLASELTGWQLNIMTVEESHEKSEEEAASICELF
CCCCCCEEEECHHHHHHHHCCCCCCEEEHHHCCCCEEEEEEECCHHCCCHHHHHHHHHHH
MEKLDVDEEVGRILVDEGFSTLEEIAYIPIEEMLEIQEFDHETIEELRNRARNVLLTAAI
HHHCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VTEEKIGHASEALLALEGMDIDLVRELAAKGISTQEELADLAADDLVELTGVDLDRASKI
HHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHCCCEE
IIKAREPWFT
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]