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The map label for this gene is truB [H]

Identifier: 114331630

GI number: 114331630

Start: 1727382

End: 1728371

Strand: Reverse

Name: truB [H]

Synonym: Neut_1644

Alternate gene names: 114331630

Gene position: 1728371-1727382 (Counterclockwise)

Preceding gene: 114331631

Following gene: 114331629

Centisome position: 64.95

GC content: 47.68

Gene sequence:

>990_bases
TTGGTCAAACTCTTATCATCAGCAAGATCTGTTCAGCTTAAATCAGTTAACTGTAATGGGGTTTTGTTACTCAACAAGCC
TGTTGGCATCTCATCTAACCGGGCTCTTCAAATCAGTAAGCGTTTGTTGTCAGCAACAAAGGCCGGTCATACTGGCACGT
TGGATCCGATGGCACAAGGCTTGCTGCCAATTTGTCTTGGTGAAGCCACCAAGTTTTCTTCAGCTTTGCTGAGCGCGGAT
AAAACCTACAAAGCCTCTCTTCAGCTTGGTTATATCAGTAGTACAGGGGATGCTGAGGGTGAAATCAGGCGGGTAGCTAA
ATCTGATGCGTGTCCACCTGATTCTCGGCAAGTAACTGCAATCCTGCAAACCTTTCTTGGCCGATCAAATCAGATTCCGC
CCATGTTCAGCGCGCTGAAGCTGTATGGCAAACCACTTTATCGATATGCACGAGAGGGAATTACAATTGAGCGCAAGGTG
CGTGAAATTGTAATTCACAGTATATCTCTCAATGCTTTGTCGGGTTTTGAGATGATGATTGCGGTCCGTTGTAGTTCAGG
CACCTATATTCGTACACTGGCAGAAGATATTGGAAAGGCGCTTGGCTATGGCGGCGCTTATCTCACCGCGCTTTCCCGTA
CTGGTGTCGGCCATTTCGATTTATCGCAAGCTTGCAATCTGGATCAGCTCGAATCCGAGCAGCCTTCTGACCGCCTGAAA
TTTTTGTATCCAATTGATAGCCTTTTAAGCGACATTCCTTCTATGGTGTTTGATGACGATGAAGTGGTGCGTTTGCAGCA
AGGGCAGAAAATCAGGAAGGACAGATCGCGCCCCCTGTTCCTGGCCAGTCCCAAATTGAAGCTATACGATAGGGAGAATA
TTTTTCTTGGTCTCGGAGAGTGGGTTGATTCCGAGGTCATCGCACCCAGAAGAATGGTTGTGTTGGCCGCTGTTACAGCA
GCAGGCGGGTTAGCTGATTCAATAGGCTAG

Upstream 100 bases:

>100_bases
TGTAGGTAAAACCTGATCCTGATTGGTGATATCTACAGAATTGGCGTTTTATGCGATATGGCGATATAACCTAGAATTAC
ATTTAGCTAGCTAAAATTTT

Downstream 100 bases:

>100_bases
AAAAAAGAGCAGAATGATTTTTATATATCCAAGCACTGGCAGCACCACTAAGAAATCAGCAATACGCTATAATCTCCAAC
TTTTATGATTGCGGCTCAAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MVKLLSSARSVQLKSVNCNGVLLLNKPVGISSNRALQISKRLLSATKAGHTGTLDPMAQGLLPICLGEATKFSSALLSAD
KTYKASLQLGYISSTGDAEGEIRRVAKSDACPPDSRQVTAILQTFLGRSNQIPPMFSALKLYGKPLYRYAREGITIERKV
REIVIHSISLNALSGFEMMIAVRCSSGTYIRTLAEDIGKALGYGGAYLTALSRTGVGHFDLSQACNLDQLESEQPSDRLK
FLYPIDSLLSDIPSMVFDDDEVVRLQQGQKIRKDRSRPLFLASPKLKLYDRENIFLGLGEWVDSEVIAPRRMVVLAAVTA
AGGLADSIG

Sequences:

>Translated_329_residues
MVKLLSSARSVQLKSVNCNGVLLLNKPVGISSNRALQISKRLLSATKAGHTGTLDPMAQGLLPICLGEATKFSSALLSAD
KTYKASLQLGYISSTGDAEGEIRRVAKSDACPPDSRQVTAILQTFLGRSNQIPPMFSALKLYGKPLYRYAREGITIERKV
REIVIHSISLNALSGFEMMIAVRCSSGTYIRTLAEDIGKALGYGGAYLTALSRTGVGHFDLSQACNLDQLESEQPSDRLK
FLYPIDSLLSDIPSMVFDDDEVVRLQQGQKIRKDRSRPLFLASPKLKLYDRENIFLGLGEWVDSEVIAPRRMVVLAAVTA
AGGLADSIG
>Mature_329_residues
MVKLLSSARSVQLKSVNCNGVLLLNKPVGISSNRALQISKRLLSATKAGHTGTLDPMAQGLLPICLGEATKFSSALLSAD
KTYKASLQLGYISSTGDAEGEIRRVAKSDACPPDSRQVTAILQTFLGRSNQIPPMFSALKLYGKPLYRYAREGITIERKV
REIVIHSISLNALSGFEMMIAVRCSSGTYIRTLAEDIGKALGYGGAYLTALSRTGVGHFDLSQACNLDQLESEQPSDRLK
FLYPIDSLLSDIPSMVFDDDEVVRLQQGQKIRKDRSRPLFLASPKLKLYDRENIFLGLGEWVDSEVIAPRRMVVLAAVTA
AGGLADSIG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=234, Percent_Identity=32.4786324786325, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI2367200, Length=309, Percent_Identity=41.4239482200647, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17553978, Length=272, Percent_Identity=24.6323529411765, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324037, Length=192, Percent_Identity=30.7291666666667, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=282, Percent_Identity=26.5957446808511, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364189, Length=279, Percent_Identity=25.089605734767, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364187, Length=279, Percent_Identity=25.089605734767, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364185, Length=279, Percent_Identity=25.089605734767, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364183, Length=279, Percent_Identity=25.089605734767, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI62471759, Length=279, Percent_Identity=25.089605734767, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17975520, Length=279, Percent_Identity=25.089605734767, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35727; Mature: 35727

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLLSSARSVQLKSVNCNGVLLLNKPVGISSNRALQISKRLLSATKAGHTGTLDPMAQG
CCCHHCCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHC
LLPICLGEATKFSSALLSADKTYKASLQLGYISSTGDAEGEIRRVAKSDACPPDSRQVTA
CHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHH
ILQTFLGRSNQIPPMFSALKLYGKPLYRYAREGITIERKVREIVIHSISLNALSGFEMMI
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCEEEE
AVRCSSGTYIRTLAEDIGKALGYGGAYLTALSRTGVGHFDLSQACNLDQLESEQPSDRLK
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCCHHHCCCCCCCHHE
FLYPIDSLLSDIPSMVFDDDEVVRLQQGQKIRKDRSRPLFLASPKLKLYDRENIFLGLGE
EEECHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCCCEEEECCCEEEEECCCEEEECCC
WVDSEVIAPRRMVVLAAVTAAGGLADSIG
CCCCCCCCCHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MVKLLSSARSVQLKSVNCNGVLLLNKPVGISSNRALQISKRLLSATKAGHTGTLDPMAQG
CCCHHCCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHC
LLPICLGEATKFSSALLSADKTYKASLQLGYISSTGDAEGEIRRVAKSDACPPDSRQVTA
CHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHH
ILQTFLGRSNQIPPMFSALKLYGKPLYRYAREGITIERKVREIVIHSISLNALSGFEMMI
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCEEEE
AVRCSSGTYIRTLAEDIGKALGYGGAYLTALSRTGVGHFDLSQACNLDQLESEQPSDRLK
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCCHHHCCCCCCCHHE
FLYPIDSLLSDIPSMVFDDDEVVRLQQGQKIRKDRSRPLFLASPKLKLYDRENIFLGLGE
EEECHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCCCEEEECCCEEEEECCCEEEECCC
WVDSEVIAPRRMVVLAAVTAAGGLADSIG
CCCCCCCCCHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]