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The map label for this gene is rlmB [H]

Identifier: 114331604

GI number: 114331604

Start: 1699787

End: 1700569

Strand: Reverse

Name: rlmB [H]

Synonym: Neut_1618

Alternate gene names: 114331604

Gene position: 1700569-1699787 (Counterclockwise)

Preceding gene: 114331605

Following gene: 114331591

Centisome position: 63.91

GC content: 50.19

Gene sequence:

>783_bases
ATGTCTCATTCCCAGTATATTTTTGGCTTCCATGCCATTACCAGCCGTTTACGGCAACATCCGGACAGCATCAAGGAAAT
TTATCTGGATACCAATCGACAGGATCAGCGTGCGCGTGATTTGATGAATCTGGCTGAGACCACTTCCTCACGTCTGATTC
TCTGTAATCAGGATCGACTTTGTAAAATGACCGGTACGACTCGTCACCAGGGTGTGGCAGCCCATGTGATGAAGTTGCAG
CAGTACACTGCACTTGAAGATTTACTGGATGGATTGACCGAGCCGGCATTTCTGCTGGTGCTGGATGGCGTGAAGGATCC
GCACAATCTGGGGGCGTGTTTGCGTGTTGCCGATGCGTTTGGTGTGCATGCGGTGATAGTCCCCAAGGATAGGGCAGTCG
GTTTATCGGCAACGGTTCATAAAGTTGCCAGTGGTGCAGTGGATACTGTACCCTTTTTTGCTGTGACCAATCTGGCCAGA
ACCTTGCGTGAACTGAAGGAGGAGGGCATCTGGATCGTAGGTACAACTGCTGATGCATCCGATACACTTGATTCAATTAC
GCTGACAAGACCTATTGCCTGGGTACTGGGTGCCGAGGGTGAGGGAATGCGTCGCCTGACTCGGGAAACCTGCGATCAGC
TTGTAAGTATCCCGATGAGCGGCAGTATCGAAAGTTTGAATGTATCAGTCAGTGCCGGCATATGCCTGTTTGAAACCTGC
CGCCAGCATGTTCAAAAGGCAAAAACTGGGTTGAACTCACTTTCTGCGAAAGAAGTTGTTTGA

Upstream 100 bases:

>100_bases
CGATCTGGCCACCCGCAAGATAGATTTTGTCCTTGCAGACAATAACAATGCACGCAAGAAATCAAGCAAATAATATTCTT
CTTCATTTCTCATCGCCATC

Downstream 100 bases:

>100_bases
ACTGCGGGTGTGGTGGTTTGAGGTTAACTGCATATCCGATTCCTGTTCTATGGATGAGTCCAGTCCTTGTCAGGGTTGAT
TTTCGGTTGGGTCAGTCACA

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSHSQYIFGFHAITSRLRQHPDSIKEIYLDTNRQDQRARDLMNLAETTSSRLILCNQDRLCKMTGTTRHQGVAAHVMKLQ
QYTALEDLLDGLTEPAFLLVLDGVKDPHNLGACLRVADAFGVHAVIVPKDRAVGLSATVHKVASGAVDTVPFFAVTNLAR
TLRELKEEGIWIVGTTADASDTLDSITLTRPIAWVLGAEGEGMRRLTRETCDQLVSIPMSGSIESLNVSVSAGICLFETC
RQHVQKAKTGLNSLSAKEVV

Sequences:

>Translated_260_residues
MSHSQYIFGFHAITSRLRQHPDSIKEIYLDTNRQDQRARDLMNLAETTSSRLILCNQDRLCKMTGTTRHQGVAAHVMKLQ
QYTALEDLLDGLTEPAFLLVLDGVKDPHNLGACLRVADAFGVHAVIVPKDRAVGLSATVHKVASGAVDTVPFFAVTNLAR
TLRELKEEGIWIVGTTADASDTLDSITLTRPIAWVLGAEGEGMRRLTRETCDQLVSIPMSGSIESLNVSVSAGICLFETC
RQHVQKAKTGLNSLSAKEVV
>Mature_259_residues
SHSQYIFGFHAITSRLRQHPDSIKEIYLDTNRQDQRARDLMNLAETTSSRLILCNQDRLCKMTGTTRHQGVAAHVMKLQQ
YTALEDLLDGLTEPAFLLVLDGVKDPHNLGACLRVADAFGVHAVIVPKDRAVGLSATVHKVASGAVDTVPFFAVTNLART
LRELKEEGIWIVGTTADASDTLDSITLTRPIAWVLGAEGEGMRRLTRETCDQLVSIPMSGSIESLNVSVSAGICLFETCR
QHVQKAKTGLNSLSAKEVV

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=29.277566539924, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=53.1120331950208, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788935, Length=237, Percent_Identity=25.3164556962025, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=32.2368421052632, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17555908, Length=259, Percent_Identity=26.2548262548263, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=169, Percent_Identity=28.4023668639053, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28410; Mature: 28279

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSQYIFGFHAITSRLRQHPDSIKEIYLDTNRQDQRARDLMNLAETTSSRLILCNQDRL
CCCCCEEHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCE
CKMTGTTRHQGVAAHVMKLQQYTALEDLLDGLTEPAFLLVLDGVKDPHNLGACLRVADAF
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GVHAVIVPKDRAVGLSATVHKVASGAVDTVPFFAVTNLARTLRELKEEGIWIVGTTADAS
CEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
DTLDSITLTRPIAWVLGAEGEGMRRLTRETCDQLVSIPMSGSIESLNVSVSAGICLFETC
HHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHH
RQHVQKAKTGLNSLSAKEVV
HHHHHHHHHCHHHCHHHCCC
>Mature Secondary Structure 
SHSQYIFGFHAITSRLRQHPDSIKEIYLDTNRQDQRARDLMNLAETTSSRLILCNQDRL
CCCCEEHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCE
CKMTGTTRHQGVAAHVMKLQQYTALEDLLDGLTEPAFLLVLDGVKDPHNLGACLRVADAF
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GVHAVIVPKDRAVGLSATVHKVASGAVDTVPFFAVTNLARTLRELKEEGIWIVGTTADAS
CEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
DTLDSITLTRPIAWVLGAEGEGMRRLTRETCDQLVSIPMSGSIESLNVSVSAGICLFETC
HHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHH
RQHVQKAKTGLNSLSAKEVV
HHHHHHHHHCHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]