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The map label for this gene is thiD [H]

Identifier: 114331526

GI number: 114331526

Start: 1609008

End: 1609850

Strand: Reverse

Name: thiD [H]

Synonym: Neut_1539

Alternate gene names: 114331526

Gene position: 1609850-1609008 (Counterclockwise)

Preceding gene: 114331530

Following gene: 114331525

Centisome position: 60.5

GC content: 48.64

Gene sequence:

>843_bases
ATGTCACTACCACCTCCAATTGTACTATCCTTCGCTGCCAGTGACCCAAGCGGGGGCGCCGGTATTCAGGCGGATATACT
GACTCTTGCCGGAATGGGCTGTCATCCACTCACTGTACTCACAGCCGTAACTGTCCAGGATACAACCGGTGTCGAGGACA
TATTTGTGATGGATGCTGAATGGGTCATTGATCAGGCAAGAACCGTGTTGCAAGATATGCCGGTACAAGCATTCAAGATT
GGGATGCTGGGCAGTGTTGAAATTGTCGCAGCTATTGCTGAAGTTATATCGGATTATCCGGATATTCCACTGGTACTTGA
TCCAGTACTGGCTTCCAGTCGTGGCGACATGTTTGCCAATGAAGAAGTAATTTCTGCCATGCGTGAGATGTTATTTTCCC
AGGCGACAATTATTACCCCTAACAGTTTTGAGGCCAGGCGTATTGCGCTTGATCACGAAGATGATCCGGAAATACTGGGT
TTGAAGCAAAGTGCAGATCAATTGTTGCAATGGGGTTGCAGTTACGTGCTTATCAAGGGAGCTCATGAAAATACACCGCA
AGTGGTTAACGTACTTTACGATGTCAATGGTATCGTCCGGTCCGATGCATGGCAGCGCTTGCCTGGATCGTTTCATGGCT
CAGGGTGCACCTTGTCATCCGCAATAGCGGCTTCACTTGCTCATGGTATGTCAATTACGGACAGCGTCTACGAGGCACAG
GAATTTACTTGGCATACGCTCAAGGCAGGCTTCCGCCCCGGAATGGGACAATATATACCAGACAGATTTTTTTGGATGGA
AAGTGATAATTTGGAAGAGGAAGCCGGCGGCTCCGATGATTGA

Upstream 100 bases:

>100_bases
AATCATGCACATATAACGGTAATAATCCTGGTTACTTTCTGTAGTCATAATAGATAAGTAGATTTGCTTAAGTTAAAATA
ACATTTTACGGTATTAATGC

Downstream 100 bases:

>100_bases
TCCTCCTATGGATCGGATTAAGGTGAGCGGCCTTTATGCAATCACGCCTGATATTGAAAATATCGATCGGCTGTGTGAGA
TGGCTTATCACGTATTGGCT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSLPPPIVLSFAASDPSGGAGIQADILTLAGMGCHPLTVLTAVTVQDTTGVEDIFVMDAEWVIDQARTVLQDMPVQAFKI
GMLGSVEIVAAIAEVISDYPDIPLVLDPVLASSRGDMFANEEVISAMREMLFSQATIITPNSFEARRIALDHEDDPEILG
LKQSADQLLQWGCSYVLIKGAHENTPQVVNVLYDVNGIVRSDAWQRLPGSFHGSGCTLSSAIAASLAHGMSITDSVYEAQ
EFTWHTLKAGFRPGMGQYIPDRFFWMESDNLEEEAGGSDD

Sequences:

>Translated_280_residues
MSLPPPIVLSFAASDPSGGAGIQADILTLAGMGCHPLTVLTAVTVQDTTGVEDIFVMDAEWVIDQARTVLQDMPVQAFKI
GMLGSVEIVAAIAEVISDYPDIPLVLDPVLASSRGDMFANEEVISAMREMLFSQATIITPNSFEARRIALDHEDDPEILG
LKQSADQLLQWGCSYVLIKGAHENTPQVVNVLYDVNGIVRSDAWQRLPGSFHGSGCTLSSAIAASLAHGMSITDSVYEAQ
EFTWHTLKAGFRPGMGQYIPDRFFWMESDNLEEEAGGSDD
>Mature_279_residues
SLPPPIVLSFAASDPSGGAGIQADILTLAGMGCHPLTVLTAVTVQDTTGVEDIFVMDAEWVIDQARTVLQDMPVQAFKIG
MLGSVEIVAAIAEVISDYPDIPLVLDPVLASSRGDMFANEEVISAMREMLFSQATIITPNSFEARRIALDHEDDPEILGL
KQSADQLLQWGCSYVLIKGAHENTPQVVNVLYDVNGIVRSDAWQRLPGSFHGSGCTLSSAIAASLAHGMSITDSVYEAQE
FTWHTLKAGFRPGMGQYIPDRFFWMESDNLEEEAGGSDD

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=236, Percent_Identity=35.1694915254237, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325378, Length=261, Percent_Identity=30.2681992337165, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324517, Length=258, Percent_Identity=32.1705426356589, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324997, Length=262, Percent_Identity=30.9160305343511, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30235; Mature: 30104

Theoretical pI: Translated: 3.98; Mature: 3.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPPPIVLSFAASDPSGGAGIQADILTLAGMGCHPLTVLTAVTVQDTTGVEDIFVMDAE
CCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHEEEEECCCCCCCEEEEECHH
WVIDQARTVLQDMPVQAFKIGMLGSVEIVAAIAEVISDYPDIPLVLDPVLASSRGDMFAN
HHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCCCC
EEVISAMREMLFSQATIITPNSFEARRIALDHEDDPEILGLKQSADQLLQWGCSYVLIKG
HHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCCCCCEECCHHHHHHHHHCCCCEEEEEC
AHENTPQVVNVLYDVNGIVRSDAWQRLPGSFHGSGCTLSSAIAASLAHGMSITDSVYEAQ
CCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
EFTWHTLKAGFRPGMGQYIPDRFFWMESDNLEEEAGGSDD
HHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHCCCCCCC
>Mature Secondary Structure 
SLPPPIVLSFAASDPSGGAGIQADILTLAGMGCHPLTVLTAVTVQDTTGVEDIFVMDAE
CCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHEEEEECCCCCCCEEEEECHH
WVIDQARTVLQDMPVQAFKIGMLGSVEIVAAIAEVISDYPDIPLVLDPVLASSRGDMFAN
HHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCCCC
EEVISAMREMLFSQATIITPNSFEARRIALDHEDDPEILGLKQSADQLLQWGCSYVLIKG
HHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCCCCCEECCHHHHHHHHHCCCCEEEEEC
AHENTPQVVNVLYDVNGIVRSDAWQRLPGSFHGSGCTLSSAIAASLAHGMSITDSVYEAQ
CCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
EFTWHTLKAGFRPGMGQYIPDRFFWMESDNLEEEAGGSDD
HHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]