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The map label for this gene is thiE

Identifier: 114331525

GI number: 114331525

Start: 1608377

End: 1609015

Strand: Reverse

Name: thiE

Synonym: Neut_1538

Alternate gene names: 114331525

Gene position: 1609015-1608377 (Counterclockwise)

Preceding gene: 114331526

Following gene: 114331524

Centisome position: 60.47

GC content: 47.1

Gene sequence:

>639_bases
ATGATTGATCCTCCTATGGATCGGATTAAGGTGAGCGGCCTTTATGCAATCACGCCTGATATTGAAAATATCGATCGGCT
GTGTGAGATGGCTTATCACGTATTGGCTGGCGGCGTAAGCTGGCTTCAGTACAGGAATAAAAAGGCCAACAGTCGGCTGC
GATTGATGCAGGCCCTGGAAATTCATCTTCTCTGCAAGCAGTTTCAAGTTCCCCTGATTATTAATGATCATATGGATTTA
GTCATGGAGATCGATGCCGAAGGTTTGCACGTGGGTGGAGAAGATACTTCTGTGGCGGCAGCTAGACACTATCTCGGTCG
GAATAAAATCATTGGCGTCTCCTGCTACAATCAGCTCAGTCATGCAATTGAAGCTGAAAAGGCGGGAGCAGATTATGTTG
CATTTGGTGCTTTTTATCCGTCAATGACTAAAGTTGATGCATATCAGGCGCCGATTCATCTGCTTGATGCTGCAAAAAAA
ACGTTGAATATTCCCATTGTGGCAATCGGCGGCATCAATCTGGACAATGCCGAGGCGCTGATTGCCCGTGGATGTGATGC
CGTTGCTGTCAGTCAGGCGCTGTTCAGCGCACAAGATATCCAATCTGCTGCCCGGCATTTTTCAAAGCTATTCTGTTGA

Upstream 100 bases:

>100_bases
ACGCTCAAGGCAGGCTTCCGCCCCGGAATGGGACAATATATACCAGACAGATTTTTTTGGATGGAAAGTGATAATTTGGA
AGAGGAAGCCGGCGGCTCCG

Downstream 100 bases:

>100_bases
ACCATCAATTTCGATAACTTTTAAGCAAATATTCCAATGACGATCACTAATCAACAATTGTTCGAGCGCTCCAGGCAATA
CATTCCGGGAGGCGTTAATT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIDPPMDRIKVSGLYAITPDIENIDRLCEMAYHVLAGGVSWLQYRNKKANSRLRLMQALEIHLLCKQFQVPLIINDHMDL
VMEIDAEGLHVGGEDTSVAAARHYLGRNKIIGVSCYNQLSHAIEAEKAGADYVAFGAFYPSMTKVDAYQAPIHLLDAAKK
TLNIPIVAIGGINLDNAEALIARGCDAVAVSQALFSAQDIQSAARHFSKLFC

Sequences:

>Translated_212_residues
MIDPPMDRIKVSGLYAITPDIENIDRLCEMAYHVLAGGVSWLQYRNKKANSRLRLMQALEIHLLCKQFQVPLIINDHMDL
VMEIDAEGLHVGGEDTSVAAARHYLGRNKIIGVSCYNQLSHAIEAEKAGADYVAFGAFYPSMTKVDAYQAPIHLLDAAKK
TLNIPIVAIGGINLDNAEALIARGCDAVAVSQALFSAQDIQSAARHFSKLFC
>Mature_212_residues
MIDPPMDRIKVSGLYAITPDIENIDRLCEMAYHVLAGGVSWLQYRNKKANSRLRLMQALEIHLLCKQFQVPLIINDHMDL
VMEIDAEGLHVGGEDTSVAAARHYLGRNKIIGVSCYNQLSHAIEAEKAGADYVAFGAFYPSMTKVDAYQAPIHLLDAAKK
TLNIPIVAIGGINLDNAEALIARGCDAVAVSQALFSAQDIQSAARHFSKLFC

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=194, Percent_Identity=29.8969072164948, Blast_Score=71, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=204, Percent_Identity=31.3725490196078, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_NITEC (Q0AFV0)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747747.1
- ProteinModelPortal:   Q0AFV0
- STRING:   Q0AFV0
- GeneID:   4273619
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1538
- NMPDR:   fig|335283.3.peg.1820
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- PhylomeDB:   Q0AFV0
- ProtClustDB:   PRK00043
- BioCyc:   NEUT335283:NEUT_1538-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23225; Mature: 23225

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPPMDRIKVSGLYAITPDIENIDRLCEMAYHVLAGGVSWLQYRNKKANSRLRLMQALE
CCCCCCHHEEECEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
IHLLCKQFQVPLIINDHMDLVMEIDAEGLHVGGEDTSVAAARHYLGRNKIIGVSCYNQLS
HHHHHHHHCCCEEECCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHH
HAIEAEKAGADYVAFGAFYPSMTKVDAYQAPIHLLDAAKKTLNIPIVAIGGINLDNAEAL
HHHHHHHCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHH
IARGCDAVAVSQALFSAQDIQSAARHFSKLFC
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDPPMDRIKVSGLYAITPDIENIDRLCEMAYHVLAGGVSWLQYRNKKANSRLRLMQALE
CCCCCCHHEEECEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
IHLLCKQFQVPLIINDHMDLVMEIDAEGLHVGGEDTSVAAARHYLGRNKIIGVSCYNQLS
HHHHHHHHCCCEEECCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHH
HAIEAEKAGADYVAFGAFYPSMTKVDAYQAPIHLLDAAKKTLNIPIVAIGGINLDNAEAL
HHHHHHHCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHH
IARGCDAVAVSQALFSAQDIQSAARHFSKLFC
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA