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The map label for this gene is queA

Identifier: 114331419

GI number: 114331419

Start: 1511065

End: 1512105

Strand: Direct

Name: queA

Synonym: Neut_1430

Alternate gene names: 114331419

Gene position: 1511065-1512105 (Clockwise)

Preceding gene: 114331414

Following gene: 114331420

Centisome position: 56.78

GC content: 48.9

Gene sequence:

>1041_bases
ATGAAAACTGATGATTTTGATTTTTCCCTTCCTAATGAGCTAATTGCACAGCTTCCACTCGCAAACCGGGCAGATAGCCG
AATGCTGTATATAAACAGCCGTCAATCTGATTTACGTGATGCGGCTTTCAAGGATCTGCCTGCTTACCTTAAACAAGGTG
ATGTGATCGTGTTCAACAATACTCGTGTTGTCAAAGCCCGGTTGGCAGGCTTCAAATCCACCGGCGGCAAGGTCGAAGTC
ATGATTGAGCGTATTCTGGGCACACATCAGGCACGAGCATTGATCCGCGCCAGTCATGCGCCGGCGATTGGTTCCACGTT
ATTGCTGGAAAATGCGATTACCGTGCAGGTAGAAGCACGTGAACAGGATATTTACACACTGCGCTTCATACACTCACAGC
CACTCATCGAATTGCTTGATCAATATGGACACACTCCGCTACCTCCCTATATTGGACGCATAGCCACAGCTTCTGATGAA
AGTCGTTATCAGACTGTTTTCGCCCAAGAAACTGGAGCGGTTGCAGCCCCCACTGCAGGCTTGCATTTTGATGAAACCAT
GCTGACAACATTACAGACTCTGGGGATAAAAATTGCTTGGGTCACCTTGCACGTCGGCGCTGGAACTTTCCAGCCAGTGC
GCGTGGAAAATATCAATCAGCACATTATGCATACCGAGCAATACCATATCCCGACAGAAACGATAGAAATTATACGGAGA
TGCAAGGCCGGTGGTGGAAGTGTGCTCGCAGTTGGCACCACCAGCTTGCGTGCACTGGAAGCCAGCGCGTTGATTGGCGA
TGGCGAGCTGGTTGCTGGATCGAACGAAACCAATCTGTTTATTACTCCCGGATTTCAGTTCCGCGTTGTAGACCGACTGC
TGACCAATTTTCATCTGCCTCGCTCCACCTTATTGATGCTGGTATCCGCTTTTGCTGGCATAGAGACTATTCGTCATGCC
TATCAGCACGCCGTTAATAATCATTATCGTTTTTTCAGTTACGGGGATGCCATGCTGATTGAGGGCGATAGCCGATCATG
A

Upstream 100 bases:

>100_bases
CCATTCTGACTGGCAGAAAGCTCCTTGCAAAAACCTCTCATTGCGACACTCTTTCCTAAAAAATACAGGCCCTTTACTGG
TCACCGGTATAATCCGCAGC

Downstream 100 bases:

>100_bases
AATTTCAGCTGCATTGCCACGACCATCACGCCAGACGAGGAACGTTGACGCTTGCCCATGGCATGATAGAAACACCGGCC
TTCATGCCGGTTGGCACTTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKTDDFDFSLPNELIAQLPLANRADSRMLYINSRQSDLRDAAFKDLPAYLKQGDVIVFNNTRVVKARLAGFKSTGGKVEV
MIERILGTHQARALIRASHAPAIGSTLLLENAITVQVEAREQDIYTLRFIHSQPLIELLDQYGHTPLPPYIGRIATASDE
SRYQTVFAQETGAVAAPTAGLHFDETMLTTLQTLGIKIAWVTLHVGAGTFQPVRVENINQHIMHTEQYHIPTETIEIIRR
CKAGGGSVLAVGTTSLRALEASALIGDGELVAGSNETNLFITPGFQFRVVDRLLTNFHLPRSTLLMLVSAFAGIETIRHA
YQHAVNNHYRFFSYGDAMLIEGDSRS

Sequences:

>Translated_346_residues
MKTDDFDFSLPNELIAQLPLANRADSRMLYINSRQSDLRDAAFKDLPAYLKQGDVIVFNNTRVVKARLAGFKSTGGKVEV
MIERILGTHQARALIRASHAPAIGSTLLLENAITVQVEAREQDIYTLRFIHSQPLIELLDQYGHTPLPPYIGRIATASDE
SRYQTVFAQETGAVAAPTAGLHFDETMLTTLQTLGIKIAWVTLHVGAGTFQPVRVENINQHIMHTEQYHIPTETIEIIRR
CKAGGGSVLAVGTTSLRALEASALIGDGELVAGSNETNLFITPGFQFRVVDRLLTNFHLPRSTLLMLVSAFAGIETIRHA
YQHAVNNHYRFFSYGDAMLIEGDSRS
>Mature_346_residues
MKTDDFDFSLPNELIAQLPLANRADSRMLYINSRQSDLRDAAFKDLPAYLKQGDVIVFNNTRVVKARLAGFKSTGGKVEV
MIERILGTHQARALIRASHAPAIGSTLLLENAITVQVEAREQDIYTLRFIHSQPLIELLDQYGHTPLPPYIGRIATASDE
SRYQTVFAQETGAVAAPTAGLHFDETMLTTLQTLGIKIAWVTLHVGAGTFQPVRVENINQHIMHTEQYHIPTETIEIIRR
CKAGGGSVLAVGTTSLRALEASALIGDGELVAGSNETNLFITPGFQFRVVDRLLTNFHLPRSTLLMLVSAFAGIETIRHA
YQHAVNNHYRFFSYGDAMLIEGDSRS

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=343, Percent_Identity=48.9795918367347, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_NITEC (Q0AG56)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747641.1
- ProteinModelPortal:   Q0AG56
- SMR:   Q0AG56
- STRING:   Q0AG56
- GeneID:   4272734
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1430
- NMPDR:   fig|335283.3.peg.546
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q0AG56
- ProtClustDB:   PRK00147
- BioCyc:   NEUT335283:NEUT_1430-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38257; Mature: 38257

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDDFDFSLPNELIAQLPLANRADSRMLYINSRQSDLRDAAFKDLPAYLKQGDVIVFNN
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECC
TRVVKARLAGFKSTGGKVEVMIERILGTHQARALIRASHAPAIGSTLLLENAITVQVEAR
CEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEECCEEEEEEEEC
EQDIYTLRFIHSQPLIELLDQYGHTPLPPYIGRIATASDESRYQTVFAQETGAVAAPTAG
CCCEEEEEEECCCHHHHHHHHHCCCCCCHHHCCEECCCCCCHHEEEEECCCCCEECCCCC
LHFDETMLTTLQTLGIKIAWVTLHVGAGTFQPVRVENINQHIMHTEQYHIPTETIEIIRR
CCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCEECCCCHHHHHCCCCCCCCHHHHHHHHH
CKAGGGSVLAVGTTSLRALEASALIGDGELVAGSNETNLFITPGFQFRVVDRLLTNFHLP
HCCCCCCEEEEECCHHHHHHHHEEECCCCEEECCCCCEEEEECCCHHHHHHHHHHHCCCC
RSTLLMLVSAFAGIETIRHAYQHAVNNHYRFFSYGDAMLIEGDSRS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCC
>Mature Secondary Structure
MKTDDFDFSLPNELIAQLPLANRADSRMLYINSRQSDLRDAAFKDLPAYLKQGDVIVFNN
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECC
TRVVKARLAGFKSTGGKVEVMIERILGTHQARALIRASHAPAIGSTLLLENAITVQVEAR
CEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEECCEEEEEEEEC
EQDIYTLRFIHSQPLIELLDQYGHTPLPPYIGRIATASDESRYQTVFAQETGAVAAPTAG
CCCEEEEEEECCCHHHHHHHHHCCCCCCHHHCCEECCCCCCHHEEEEECCCCCEECCCCC
LHFDETMLTTLQTLGIKIAWVTLHVGAGTFQPVRVENINQHIMHTEQYHIPTETIEIIRR
CCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCEECCCCHHHHHCCCCCCCCHHHHHHHHH
CKAGGGSVLAVGTTSLRALEASALIGDGELVAGSNETNLFITPGFQFRVVDRLLTNFHLP
HCCCCCCEEEEECCHHHHHHHHEEECCCCEEECCCCCEEEEECCCHHHHHHHHHHHCCCC
RSTLLMLVSAFAGIETIRHAYQHAVNNHYRFFSYGDAMLIEGDSRS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA