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The map label for this gene is tgt [H]

Identifier: 114331420

GI number: 114331420

Start: 1512102

End: 1513187

Strand: Direct

Name: tgt [H]

Synonym: Neut_1431

Alternate gene names: 114331420

Gene position: 1512102-1513187 (Clockwise)

Preceding gene: 114331419

Following gene: 114331421

Centisome position: 56.82

GC content: 52.95

Gene sequence:

>1086_bases
ATGAAATTTCAGCTGCATTGCCACGACCATCACGCCAGACGAGGAACGTTGACGCTTGCCCATGGCATGATAGAAACACC
GGCCTTCATGCCGGTTGGCACTTATGGTGCAGTCAAAGGATTATCTCCAGACGAGCTACAAACCCTGGGGGCCGAAATCA
TTCTGGGCAATACTTTTCATTTATGGTTGCGCCCCGGGCTAGAAGTGATTGAAGCGCATGGCGGATTGCACCGGTTTATG
CACTGGGACGGGCCTATCTTGACCGATTCAGGCGGATTCCAGGTATTCAGCCTGGGCGCGTTACGCAAAATCAGCGAAGA
AGGGGTACGTTTCCGCTCGCCAGTCAATGGCGATACCTGCTTTCTGACACCGGAGGAGTCCATGCGCATCCAGCGTGTGC
TGAATTCGGATATTGTCATGATTTTCGATGAATGCACCCCCTATCCGGTCGATATGCAAATTGCCGAAGATTCCATGCAA
TTGAGTCTGCGCTGGGCTGAGCGCTCGAAAGCAGCGCATGCCGGTAATCCAAACGCACTGTTCGGTATCGTGCAAGGTGG
CATGTACGAATCGCTGCGCGATCGCTCAATAGCCGGTTTGTATAATATTGGTTTTGATGGATACGCTATTGGCGGATTAT
CCGTAGGCGAACCCAAGCTGGAAATGCAGCGGATCCTCAGCCATACCGCGCCACAGCTGCCGGTAGACAAGCCACGTTAT
CTGATGGGGGTAGGCACGCCTGAGGATATTGTTCGTGCTGTTGAACAGGGTATTGATATGTTCGATTGCGTGCTGCCTAC
CCGCAATGCACGCAATGGCTGGCTCTATACCAGCCAAGGTGTTCTCAAGCTGCGCAACAGCCGTTACCGGCTGGATACCT
CCCCGCCGGATGAAAATTGCGATTGCTATACCTGTCGCCATTTTACCCGTGCCTATCTGCATCATCTGCAGCGCACCGGA
GAAATGCTCGGCGCCCGGCTCAACTCGCTGCATAACTTACACTACTACCAACGGCTCATGTCTGATATCCGTAAGGCGAT
TGAAGCTGGTCAGTTTGAACAGTTTGCATGCAGATTTTCCGGCTAG

Upstream 100 bases:

>100_bases
GCATAGAGACTATTCGTCATGCCTATCAGCACGCCGTTAATAATCATTATCGTTTTTTCAGTTACGGGGATGCCATGCTG
ATTGAGGGCGATAGCCGATC

Downstream 100 bases:

>100_bases
AACTTCATGCTAAAATACATAACGATTCAGTTTCCATTCATACCCAATTGAAGGAGAAGACCTTATGCTCATAAAAGAAG
CTTTCGCCCAGGCCGCCAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKFQLHCHDHHARRGTLTLAHGMIETPAFMPVGTYGAVKGLSPDELQTLGAEIILGNTFHLWLRPGLEVIEAHGGLHRFM
HWDGPILTDSGGFQVFSLGALRKISEEGVRFRSPVNGDTCFLTPEESMRIQRVLNSDIVMIFDECTPYPVDMQIAEDSMQ
LSLRWAERSKAAHAGNPNALFGIVQGGMYESLRDRSIAGLYNIGFDGYAIGGLSVGEPKLEMQRILSHTAPQLPVDKPRY
LMGVGTPEDIVRAVEQGIDMFDCVLPTRNARNGWLYTSQGVLKLRNSRYRLDTSPPDENCDCYTCRHFTRAYLHHLQRTG
EMLGARLNSLHNLHYYQRLMSDIRKAIEAGQFEQFACRFSG

Sequences:

>Translated_361_residues
MKFQLHCHDHHARRGTLTLAHGMIETPAFMPVGTYGAVKGLSPDELQTLGAEIILGNTFHLWLRPGLEVIEAHGGLHRFM
HWDGPILTDSGGFQVFSLGALRKISEEGVRFRSPVNGDTCFLTPEESMRIQRVLNSDIVMIFDECTPYPVDMQIAEDSMQ
LSLRWAERSKAAHAGNPNALFGIVQGGMYESLRDRSIAGLYNIGFDGYAIGGLSVGEPKLEMQRILSHTAPQLPVDKPRY
LMGVGTPEDIVRAVEQGIDMFDCVLPTRNARNGWLYTSQGVLKLRNSRYRLDTSPPDENCDCYTCRHFTRAYLHHLQRTG
EMLGARLNSLHNLHYYQRLMSDIRKAIEAGQFEQFACRFSG
>Mature_361_residues
MKFQLHCHDHHARRGTLTLAHGMIETPAFMPVGTYGAVKGLSPDELQTLGAEIILGNTFHLWLRPGLEVIEAHGGLHRFM
HWDGPILTDSGGFQVFSLGALRKISEEGVRFRSPVNGDTCFLTPEESMRIQRVLNSDIVMIFDECTPYPVDMQIAEDSMQ
LSLRWAERSKAAHAGNPNALFGIVQGGMYESLRDRSIAGLYNIGFDGYAIGGLSVGEPKLEMQRILSHTAPQLPVDKPRY
LMGVGTPEDIVRAVEQGIDMFDCVLPTRNARNGWLYTSQGVLKLRNSRYRLDTSPPDENCDCYTCRHFTRAYLHHLQRTG
EMLGARLNSLHNLHYYQRLMSDIRKAIEAGQFEQFACRFSG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=43.9226519337017, Blast_Score=304, Evalue=7e-83,
Organism=Escherichia coli, GI1786607, Length=361, Percent_Identity=65.3739612188366, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=42.7777777777778, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=166, Percent_Identity=29.5180722891566, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920510, Length=359, Percent_Identity=47.075208913649, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI21355311, Length=229, Percent_Identity=30.1310043668122, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 40597; Mature: 40597

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQLHCHDHHARRGTLTLAHGMIETPAFMPVGTYGAVKGLSPDELQTLGAEIILGNTFH
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGLEVIEAHGGLHRFMHWDGPILTDSGGFQVFSLGALRKISEEGVRFRSPVNGDTC
EEECCCHHHHHHCCCCEEHEECCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCCCEE
FLTPEESMRIQRVLNSDIVMIFDECTPYPVDMQIAEDSMQLSLRWAERSKAAHAGNPNAL
EECCHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCEEEEEEEHHHCCCCCCCCCCEE
FGIVQGGMYESLRDRSIAGLYNIGFDGYAIGGLSVGEPKLEMQRILSHTAPQLPVDKPRY
EEEECCCHHHHHHCCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCE
LMGVGTPEDIVRAVEQGIDMFDCVLPTRNARNGWLYTSQGVLKLRNSRYRLDTSPPDENC
EEECCCHHHHHHHHHHCCCCEEHHCCCCCCCCCEEEECCCCEEECCCCEECCCCCCCCCC
DCYTCRHFTRAYLHHLQRTGEMLGARLNSLHNLHYYQRLMSDIRKAIEAGQFEQFACRFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECC
G
C
>Mature Secondary Structure
MKFQLHCHDHHARRGTLTLAHGMIETPAFMPVGTYGAVKGLSPDELQTLGAEIILGNTFH
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGLEVIEAHGGLHRFMHWDGPILTDSGGFQVFSLGALRKISEEGVRFRSPVNGDTC
EEECCCHHHHHHCCCCEEHEECCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCCCEE
FLTPEESMRIQRVLNSDIVMIFDECTPYPVDMQIAEDSMQLSLRWAERSKAAHAGNPNAL
EECCHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCEEEEEEEHHHCCCCCCCCCCEE
FGIVQGGMYESLRDRSIAGLYNIGFDGYAIGGLSVGEPKLEMQRILSHTAPQLPVDKPRY
EEEECCCHHHHHHCCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCE
LMGVGTPEDIVRAVEQGIDMFDCVLPTRNARNGWLYTSQGVLKLRNSRYRLDTSPPDENC
EEECCCHHHHHHHHHHCCCCEEHHCCCCCCCCCEEEECCCCEEECCCCEECCCCCCCCCC
DCYTCRHFTRAYLHHLQRTGEMLGARLNSLHNLHYYQRLMSDIRKAIEAGQFEQFACRFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]