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The map label for this gene is yafV [C]

Identifier: 114331259

GI number: 114331259

Start: 1321090

End: 1321953

Strand: Reverse

Name: yafV [C]

Synonym: Neut_1264

Alternate gene names: 114331259

Gene position: 1321953-1321090 (Counterclockwise)

Preceding gene: 114331260

Following gene: 114331258

Centisome position: 49.68

GC content: 49.65

Gene sequence:

>864_bases
ATGTCGACTAGTATAAATGTTGAATTGACTACTTCTCCCAGTTCAGGTGTTGATTCCAAAGTACGGGTGGCAGCCGTACA
GATGGCCTCTGGTCCCAGTGTAACAGCCAATCTTGAGGAAGCTTTTCGTCTGATTGAAGAGGCTGCTACAAAGGGTGCGC
AACTGGTGGCATTACCGGAATATTTCTGTATCATGGGAATGAAGGATACCGACAAGCTGGCGGTACGTGAGAATCCTGGA
GAAGGGGAAGTCCAGAATTTTCTGAGTGAAACTGCCAAGCGGTTTGGTATCTGGTTGGCAGGGGGGTCAGTACCTTTAGT
TTCACCTCTTTCCAATAAAGTATACAACAGCTGTCTGGTATACGATGAACAAGGTCGGCAGGTGGCACGATACGATAAGA
TCCACTTGTTTGGACTGTCACTGGGTAACGAGAATTTCGCTGAGGAAAGAACGATAGACGCAGGTAATCGGGTGGTTGCA
ATTGATTCTCCCTTTGGCCGTATGGGGTTGTCCATTTGCTACGATCTGCGTTTTCCCGAGCTATACCGCATGATGGGTAA
AGTGGATATCATTTTGGCACCTGCTGCGTTTACCGCCATTACCGGAAAAGCTCACTGGGAGACGCTTATTCGAGCTCGTG
CGATTGAGAACCAGGCTTATCTGATTGCGCCAGCGCAGGGAGGATTCCATGTGAACGGACGTGAAACCAATGGTGACAGT
ATGATTGTTGATCCATGGGGAGTAATCATTGATCGTCTTCCGCGCGGGGCCGGAGTAGTGCTCGCAGAAATCGATCGCGT
ATATCAGTCAAGTGTACGAGCGAGCCTGCCTGCACTGGAACATCGGTGCTTGCTTGCCTGCTGA

Upstream 100 bases:

>100_bases
ATTGAAAATATAAATCCGGGGCAAAATAAACTTGAAGGGAAAGGAAAGCTGAGTATAGGCAGCTTGACAATATTGATTGA
ATACAAGAGAGGGAAGAACT

Downstream 100 bases:

>100_bases
GAAAATATAGATTTGATCTGACTTATACGTTACCCTAATTTTTCTACAGGCTGCCTTCATTGCATGCCTCATAGCCACAG
TTTATTTATTTGCCCACTTT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSTSINVELTTSPSSGVDSKVRVAAVQMASGPSVTANLEEAFRLIEEAATKGAQLVALPEYFCIMGMKDTDKLAVRENPG
EGEVQNFLSETAKRFGIWLAGGSVPLVSPLSNKVYNSCLVYDEQGRQVARYDKIHLFGLSLGNENFAEERTIDAGNRVVA
IDSPFGRMGLSICYDLRFPELYRMMGKVDIILAPAAFTAITGKAHWETLIRARAIENQAYLIAPAQGGFHVNGRETNGDS
MIVDPWGVIIDRLPRGAGVVLAEIDRVYQSSVRASLPALEHRCLLAC

Sequences:

>Translated_287_residues
MSTSINVELTTSPSSGVDSKVRVAAVQMASGPSVTANLEEAFRLIEEAATKGAQLVALPEYFCIMGMKDTDKLAVRENPG
EGEVQNFLSETAKRFGIWLAGGSVPLVSPLSNKVYNSCLVYDEQGRQVARYDKIHLFGLSLGNENFAEERTIDAGNRVVA
IDSPFGRMGLSICYDLRFPELYRMMGKVDIILAPAAFTAITGKAHWETLIRARAIENQAYLIAPAQGGFHVNGRETNGDS
MIVDPWGVIIDRLPRGAGVVLAEIDRVYQSSVRASLPALEHRCLLAC
>Mature_286_residues
STSINVELTTSPSSGVDSKVRVAAVQMASGPSVTANLEEAFRLIEEAATKGAQLVALPEYFCIMGMKDTDKLAVRENPGE
GEVQNFLSETAKRFGIWLAGGSVPLVSPLSNKVYNSCLVYDEQGRQVARYDKIHLFGLSLGNENFAEERTIDAGNRVVAI
DSPFGRMGLSICYDLRFPELYRMMGKVDIILAPAAFTAITGKAHWETLIRARAIENQAYLIAPAQGGFHVNGRETNGDSM
IVDPWGVIIDRLPRGAGVVLAEIDRVYQSSVRASLPALEHRCLLAC

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=267, Percent_Identity=36.7041198501873, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI297632348, Length=267, Percent_Identity=36.7041198501873, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI5031947, Length=267, Percent_Identity=36.7041198501873, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI9910460, Length=268, Percent_Identity=34.3283582089552, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=32.2916666666667, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1786412, Length=216, Percent_Identity=33.7962962962963, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556280, Length=265, Percent_Identity=37.3584905660377, Blast_Score=180, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323383, Length=286, Percent_Identity=34.6153846153846, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322335, Length=296, Percent_Identity=31.0810810810811, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17933642, Length=270, Percent_Identity=35.5555555555556, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21355835, Length=276, Percent_Identity=31.8840579710145, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 31210; Mature: 31079

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSINVELTTSPSSGVDSKVRVAAVQMASGPSVTANLEEAFRLIEEAATKGAQLVALPE
CCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCEEECHHHHHHHHHHHHCCCCEEEEECC
YFCIMGMKDTDKLAVRENPGEGEVQNFLSETAKRFGIWLAGGSVPLVSPLSNKVYNSCLV
EEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCEECHHHHHHHHEEEE
YDEQGRQVARYDKIHLFGLSLGNENFAEERTIDAGNRVVAIDSPFGRMGLSICYDLRFPE
ECCCCCEEEEECEEEEEEEECCCCCCCHHCCCCCCCEEEEECCCCCCCCEEEEEECCCHH
LYRMMGKVDIILAPAAFTAITGKAHWETLIRARAIENQAYLIAPAQGGFHVNGRETNGDS
HHHHHCCEEEEEECCHHEEECCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCEECCCCE
MIVDPWGVIIDRLPRGAGVVLAEIDRVYQSSVRASLPALEHRCLLAC
EEECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCEEECC
>Mature Secondary Structure 
STSINVELTTSPSSGVDSKVRVAAVQMASGPSVTANLEEAFRLIEEAATKGAQLVALPE
CCEEEEEEEECCCCCCCCCEEEEEEEECCCCCEEECHHHHHHHHHHHHCCCCEEEEECC
YFCIMGMKDTDKLAVRENPGEGEVQNFLSETAKRFGIWLAGGSVPLVSPLSNKVYNSCLV
EEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCEECHHHHHHHHEEEE
YDEQGRQVARYDKIHLFGLSLGNENFAEERTIDAGNRVVAIDSPFGRMGLSICYDLRFPE
ECCCCCEEEEECEEEEEEEECCCCCCCHHCCCCCCCEEEEECCCCCCCCEEEEEECCCHH
LYRMMGKVDIILAPAAFTAITGKAHWETLIRARAIENQAYLIAPAQGGFHVNGRETNGDS
HHHHHCCEEEEEECCHHEEECCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCEECCCCE
MIVDPWGVIIDRLPRGAGVVLAEIDRVYQSSVRASLPALEHRCLLAC
EEECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]