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The map label for this gene is iscA [H]

Identifier: 114331238

GI number: 114331238

Start: 1298791

End: 1299120

Strand: Reverse

Name: iscA [H]

Synonym: Neut_1242

Alternate gene names: 114331238

Gene position: 1299120-1298791 (Counterclockwise)

Preceding gene: 114331239

Following gene: 114331237

Centisome position: 48.82

GC content: 42.73

Gene sequence:

>330_bases
ATGGCGATTACATTAACTGAGCGTGCGGCCAGACAGATTCAGCATCAACTGGGCAAGCGAGGTAGAGGGGTGGCGTTACG
ACTTGGAATCAGGAAATCAGGTTGTTCGGGATTCGCGTACAGTTTTGATTATGCTGACGATGTGCTGGAAGATGATCAAT
TGTTTGAATCAAATGATGCCAAGGTTATCGTTAAACGCGATCAAGTGTCTTTTATTGATGGTTCTGAGATTGATTTTGTC
CAGGACGGTCTAAACAGCTCTTTTAAATTTAGCAATCCAAATATAGACAATACTTGTGGCTGTGGAGAGAGCTTCAGCCT
TAAAACTTAG

Upstream 100 bases:

>100_bases
GAGCAGAGTCAGCGCGAACTACAGCGTGAACAGGCAGTAGTTAAATTATATGACATGCGGGAAGCTGCATTCTGATTATT
TGTTTGCAATAGAGTGAATT

Downstream 100 bases:

>100_bases
AAATTCAGGAATTATAAAAATGAGTGCTGCATTACAAAATTTGGTGAATCAACCATATAAGCACGGCTTCGTTACGAATA
TAGAATCCGATATTGCGCCT

Product: iron-sulfur cluster assembly accessory protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 109; Mature: 108

Protein sequence:

>109_residues
MAITLTERAARQIQHQLGKRGRGVALRLGIRKSGCSGFAYSFDYADDVLEDDQLFESNDAKVIVKRDQVSFIDGSEIDFV
QDGLNSSFKFSNPNIDNTCGCGESFSLKT

Sequences:

>Translated_109_residues
MAITLTERAARQIQHQLGKRGRGVALRLGIRKSGCSGFAYSFDYADDVLEDDQLFESNDAKVIVKRDQVSFIDGSEIDFV
QDGLNSSFKFSNPNIDNTCGCGESFSLKT
>Mature_108_residues
AITLTERAARQIQHQLGKRGRGVALRLGIRKSGCSGFAYSFDYADDVLEDDQLFESNDAKVIVKRDQVSFIDGSEIDFVQ
DGLNSSFKFSNPNIDNTCGCGESFSLKT

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=35.8490566037736, Blast_Score=87, Evalue=2e-18,
Organism=Homo sapiens, GI160420328, Length=84, Percent_Identity=41.6666666666667, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=47.6635514018692, Blast_Score=119, Evalue=6e-29,
Organism=Escherichia coli, GI1787974, Length=106, Percent_Identity=46.2264150943396, Blast_Score=107, Evalue=1e-25,
Organism=Escherichia coli, GI1786351, Length=108, Percent_Identity=31.4814814814815, Blast_Score=67, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25146411, Length=107, Percent_Identity=43.9252336448598, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI193209025, Length=107, Percent_Identity=43.9252336448598, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71998452, Length=123, Percent_Identity=34.1463414634146, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323001, Length=103, Percent_Identity=35.9223300970874, Blast_Score=76, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24642263, Length=107, Percent_Identity=42.9906542056075, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24649697, Length=87, Percent_Identity=34.4827586206897, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 12032; Mature: 11901

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITLTERAARQIQHQLGKRGRGVALRLGIRKSGCSGFAYSFDYADDVLEDDQLFESNDA
CEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCCHHCCCCCEECCCCC
KVIVKRDQVSFIDGSEIDFVQDGLNSSFKFSNPNIDNTCGCGESFSLKT
EEEEEECCEEEECCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AITLTERAARQIQHQLGKRGRGVALRLGIRKSGCSGFAYSFDYADDVLEDDQLFESNDA
EEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCCHHCCCCCEECCCCC
KVIVKRDQVSFIDGSEIDFVQDGLNSSFKFSNPNIDNTCGCGESFSLKT
EEEEEECCEEEECCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12775707 [H]