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The map label for this gene is sigB [H]

Identifier: 113477360

GI number: 113477360

Start: 6050404

End: 6051168

Strand: Reverse

Name: sigB [H]

Synonym: Tery_3916

Alternate gene names: 113477360

Gene position: 6051168-6050404 (Counterclockwise)

Preceding gene: 113477361

Following gene: 113477359

Centisome position: 78.08

GC content: 38.04

Gene sequence:

>765_bases
ATGTCTACCAACATCACTCAAGAACTAAAAAGTGAAACCATAAAACTGTTACAGCAATATCAACAGTTTCCTTCTTATGA
ACTTCGGAACAAATTAGTTCAACTTAATGTTGGACTTGTAAGAAAAGAAGCTTATCATTGGTTAAATAAATGTAGCGAAA
CTTATGATGATTTACTCCAAGTCGGATATATTGGATTAATTCTAGCAATTGAACGATTTGATGTTTCTAAGGCACGAGCC
TTCAGTTCTTTTGCTACTCCTTATATCAGAGGAGAAATTAAGCATTATTTAAGAGATAAAAGCCCTTCTGTACGCATCCC
CAGAAATTGGTTAGCTATAGATCGGCAGGCCACGAAAGTGATACAAGACCTGAGAATGAAGTTCCAGCGAAAACCTACAG
ATCAAGAAGTAGCTTCTGCTCTAGGTATTTCTTTAGACCAGTGGCATAAAATTCAAATGGCCTGTCGTAACCTTGCTCCT
CTGAGTTTAGATGCTCCAGTTCAAGATCAAGCTTATGGATCTACTAGTTTGGGAGAATTAGTCCCGGATAGTCAGTATCG
TAGTTTTCAGTTAGCTCAAGAAGACCAAATTCGTTTACAGCAAGCATTGTCTAAATTGGAGGAGAGCACTCGTAAGGTTT
TGGAGTTTGTTTTTCTCTGTGACTTGACTCAAAAGGAAACAGCCGAGCGAATGGGAATTAGTGCTGTTACTGTATCTCGG
AGAGTCAAAAATGGCTTAAAATGTCTGCAAAAGATAATGCAATAG

Upstream 100 bases:

>100_bases
CTAATGCTCCTTGTTTCACAGCATTTATTTGATAAATCTATTAATATAATATATATGCAATCTACGATAAGCGGTAGATT
GTATAAACGATAATATATCT

Downstream 100 bases:

>100_bases
CACTTAATGGATTAATTCCAGAATGATACTACTAATTAGCAAATAATATTTATTAGCTTATTTAATTATTTTTGGATTTT
CTATCATTCCCTAACTCTTA

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSTNITQELKSETIKLLQQYQQFPSYELRNKLVQLNVGLVRKEAYHWLNKCSETYDDLLQVGYIGLILAIERFDVSKARA
FSSFATPYIRGEIKHYLRDKSPSVRIPRNWLAIDRQATKVIQDLRMKFQRKPTDQEVASALGISLDQWHKIQMACRNLAP
LSLDAPVQDQAYGSTSLGELVPDSQYRSFQLAQEDQIRLQQALSKLEESTRKVLEFVFLCDLTQKETAERMGISAVTVSR
RVKNGLKCLQKIMQ

Sequences:

>Translated_254_residues
MSTNITQELKSETIKLLQQYQQFPSYELRNKLVQLNVGLVRKEAYHWLNKCSETYDDLLQVGYIGLILAIERFDVSKARA
FSSFATPYIRGEIKHYLRDKSPSVRIPRNWLAIDRQATKVIQDLRMKFQRKPTDQEVASALGISLDQWHKIQMACRNLAP
LSLDAPVQDQAYGSTSLGELVPDSQYRSFQLAQEDQIRLQQALSKLEESTRKVLEFVFLCDLTQKETAERMGISAVTVSR
RVKNGLKCLQKIMQ
>Mature_253_residues
STNITQELKSETIKLLQQYQQFPSYELRNKLVQLNVGLVRKEAYHWLNKCSETYDDLLQVGYIGLILAIERFDVSKARAF
SSFATPYIRGEIKHYLRDKSPSVRIPRNWLAIDRQATKVIQDLRMKFQRKPTDQEVASALGISLDQWHKIQMACRNLAPL
SLDAPVQDQAYGSTSLGELVPDSQYRSFQLAQEDQIRLQQALSKLEESTRKVLEFVFLCDLTQKETAERMGISAVTVSRR
VKNGLKCLQKIMQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=207, Percent_Identity=25.1207729468599, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788231, Length=228, Percent_Identity=29.3859649122807, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29274; Mature: 29143

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHC
>Mature Secondary Structure 
STNITQELKSETIKLLQQYQQFPSYELRNKLVQLNVGLVRKEAYHWLNKCSETYDDLLQ
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGYIGLILAIERFDVSKARAFSSFATPYIRGEIKHYLRDKSPSVRIPRNWLAIDRQATKV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IQDLRMKFQRKPTDQEVASALGISLDQWHKIQMACRNLAPLSLDAPVQDQAYGSTSLGEL
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHC
VPDSQYRSFQLAQEDQIRLQQALSKLEESTRKVLEFVFLCDLTQKETAERMGISAVTVSR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHH
RVKNGLKCLQKIMQ
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]