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The map label for this gene is potD [H]

Identifier: 113476404

GI number: 113476404

Start: 4377026

End: 4378090

Strand: Reverse

Name: potD [H]

Synonym: Tery_2816

Alternate gene names: 113476404

Gene position: 4378090-4377026 (Counterclockwise)

Preceding gene: 113476405

Following gene: 113476403

Centisome position: 56.49

GC content: 31.64

Gene sequence:

>1065_bases
ATGAAAAAAATTATTCTTTTCTTATTGATATTTACAATCGCATTAACTTTACCTATTGCTTGTAGTAATAATCCCAATAC
TTCAGAAAATAATGTTCTGAGTATATACAATTGGTCTACATATATTGCTCCTGGAGTAATGACTGAGTTTGAGAAGAAAT
TTAATGTCAAAATTCAGTACGATACTTATGAAAGTAATGAAGACTTATATGCAAAAATTAAACCTGGAAATCCTGGTTAT
GATGTGGCTTTTCCCGGTGATTACATGGTGAAAACTATGGCATCTGAAGGACTGTTAGAGGAACTTAATAAAGAGAATAT
TCCTAATATAAAAAATCTCAATAATAAGTTTACTAATCTTCCATATGATCCGGGAAATAAATATAGTATTCCTTACCAAT
TGGGTACAATGGGAATAGGTTATAATATTAAAGCAACCGGAGGTGAAATTAATAGTTGGTCAACTATGTTTGATGAGAAA
TTTAAGGGGAGAATAGCATTATTAGATGATATGCGTAGTAGCTTTGCTATTGCTTTAATTTCTCTTGGTTATAACCCGAA
TACAACTAACCCTAAGGAAATTGCTCAAGGGAAAGATTTTCTGATTAAAAATAAACAGGCGATCGCTGTTTTTGCCCCAG
ATACTGGTCAAAACTTATTAGACCAAGGTGAGGTAGATTTAACTTGTGAATGGAGTGGAGATATTTTCCAAGTTATGAAA
GAAAATGCTGACCTTCGTTACACTATTCCCAAAGAGGGAAGTATTATTTTAACTGATAATATGGTTATTCTTAAAGGAAG
TAGAAATAAAGAGCTGGCAGAAAAATTTATTAACTTTATTCTTGAACCAGAAATAGGGGCAAAAATATCTAATTTTATTA
AGTATGCTACTCCAAACCAAGCAGCAAAAGACCAAGGTTTAATTGAAGCTAATGACCTGAATAATTCTGCTATTTATCCA
CCAGCAGAATTATTTGCTAAACTTAATTATCTTCAAGATGTTGGTGAAGCTACAGTTTTGTATGATAAAGCTTGGACAGA
AGTTAAGTTAGAAGTTAGTAAATAG

Upstream 100 bases:

>100_bases
TTGAATTTGAGAAGTCTATCTCTATCATTTTTCCCCTTTGTTGAGAATTTTATCTTTCTTACTTGTGCCTTTTTTCACAA
ATATTTATCACGCTTAGTCT

Downstream 100 bases:

>100_bases
TTTTTTTTAACATTCAACTATTAATAAACTGACTCTAAAAAAACTTAGTTGATAGTTATTAATTATTAGCTTATTATTAC
ACTTCTAAGAGGAATTAGTT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKKIILFLLIFTIALTLPIACSNNPNTSENNVLSIYNWSTYIAPGVMTEFEKKFNVKIQYDTYESNEDLYAKIKPGNPGY
DVAFPGDYMVKTMASEGLLEELNKENIPNIKNLNNKFTNLPYDPGNKYSIPYQLGTMGIGYNIKATGGEINSWSTMFDEK
FKGRIALLDDMRSSFAIALISLGYNPNTTNPKEIAQGKDFLIKNKQAIAVFAPDTGQNLLDQGEVDLTCEWSGDIFQVMK
ENADLRYTIPKEGSIILTDNMVILKGSRNKELAEKFINFILEPEIGAKISNFIKYATPNQAAKDQGLIEANDLNNSAIYP
PAELFAKLNYLQDVGEATVLYDKAWTEVKLEVSK

Sequences:

>Translated_354_residues
MKKIILFLLIFTIALTLPIACSNNPNTSENNVLSIYNWSTYIAPGVMTEFEKKFNVKIQYDTYESNEDLYAKIKPGNPGY
DVAFPGDYMVKTMASEGLLEELNKENIPNIKNLNNKFTNLPYDPGNKYSIPYQLGTMGIGYNIKATGGEINSWSTMFDEK
FKGRIALLDDMRSSFAIALISLGYNPNTTNPKEIAQGKDFLIKNKQAIAVFAPDTGQNLLDQGEVDLTCEWSGDIFQVMK
ENADLRYTIPKEGSIILTDNMVILKGSRNKELAEKFINFILEPEIGAKISNFIKYATPNQAAKDQGLIEANDLNNSAIYP
PAELFAKLNYLQDVGEATVLYDKAWTEVKLEVSK
>Mature_354_residues
MKKIILFLLIFTIALTLPIACSNNPNTSENNVLSIYNWSTYIAPGVMTEFEKKFNVKIQYDTYESNEDLYAKIKPGNPGY
DVAFPGDYMVKTMASEGLLEELNKENIPNIKNLNNKFTNLPYDPGNKYSIPYQLGTMGIGYNIKATGGEINSWSTMFDEK
FKGRIALLDDMRSSFAIALISLGYNPNTTNPKEIAQGKDFLIKNKQAIAVFAPDTGQNLLDQGEVDLTCEWSGDIFQVMK
ENADLRYTIPKEGSIILTDNMVILKGSRNKELAEKFINFILEPEIGAKISNFIKYATPNQAAKDQGLIEANDLNNSAIYP
PAELFAKLNYLQDVGEATVLYDKAWTEVKLEVSK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=326, Percent_Identity=37.1165644171779, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787078, Length=353, Percent_Identity=33.7110481586402, Blast_Score=187, Evalue=7e-49,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39651; Mature: 39651

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIILFLLIFTIALTLPIACSNNPNTSENNVLSIYNWSTYIAPGVMTEFEKKFNVKIQY
CHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHEEEEEEE
DTYESNEDLYAKIKPGNPGYDVAFPGDYMVKTMASEGLLEELNKENIPNIKNLNNKFTNL
ECCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCC
PYDPGNKYSIPYQLGTMGIGYNIKATGGEINSWSTMFDEKFKGRIALLDDMRSSFAIALI
CCCCCCCEECCEEECCEEECEEEEECCCCCCCHHHHHHHHHCCEEEEEHHHHHCEEEEEE
SLGYNPNTTNPKEIAQGKDFLIKNKQAIAVFAPDTGQNLLDQGEVDLTCEWSGDIFQVMK
EECCCCCCCCHHHHHCCCCEEEECCCEEEEECCCCCCHHHHCCCEEEEEECCCHHHHHHH
ENADLRYTIPKEGSIILTDNMVILKGSRNKELAEKFINFILEPEIGAKISNFIKYATPNQ
CCCCEEEECCCCCCEEEECCEEEEECCCCHHHHHHHHHHEECCCCCHHHHHHHEECCCCC
AAKDQGLIEANDLNNSAIYPPAELFAKLNYLQDVGEATVLYDKAWTEVKLEVSK
CHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECC
>Mature Secondary Structure
MKKIILFLLIFTIALTLPIACSNNPNTSENNVLSIYNWSTYIAPGVMTEFEKKFNVKIQY
CHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHEEEEEEE
DTYESNEDLYAKIKPGNPGYDVAFPGDYMVKTMASEGLLEELNKENIPNIKNLNNKFTNL
ECCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCC
PYDPGNKYSIPYQLGTMGIGYNIKATGGEINSWSTMFDEKFKGRIALLDDMRSSFAIALI
CCCCCCCEECCEEECCEEECEEEEECCCCCCCHHHHHHHHHCCEEEEEHHHHHCEEEEEE
SLGYNPNTTNPKEIAQGKDFLIKNKQAIAVFAPDTGQNLLDQGEVDLTCEWSGDIFQVMK
EECCCCCCCCHHHHHCCCCEEEECCCEEEEECCCCCCHHHHCCCEEEEEECCCHHHHHHH
ENADLRYTIPKEGSIILTDNMVILKGSRNKELAEKFINFILEPEIGAKISNFIKYATPNQ
CCCCEEEECCCCCCEEEECCEEEEECCCCHHHHHHHHHHEECCCCCHHHHHHHEECCCCC
AAKDQGLIEANDLNNSAIYPPAELFAKLNYLQDVGEATVLYDKAWTEVKLEVSK
CHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]