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The map label for this gene is potD-B [H]

Identifier: 113476392

GI number: 113476392

Start: 4348305

End: 4349366

Strand: Reverse

Name: potD-B [H]

Synonym: Tery_2804

Alternate gene names: 113476392

Gene position: 4349366-4348305 (Counterclockwise)

Preceding gene: 113476393

Following gene: 113476391

Centisome position: 56.12

GC content: 37.85

Gene sequence:

>1062_bases
ATGTCAGCAGCTACTATCACAGTTTCAACCCTCTCTGGTTGTGGTTGGAGATTAGCTGAAGTGAAATCTAACCCCAAAAT
GCGGGGTCGTTCAGATTTACTCTATATATATACTTGGGCAGGATATACAGATCAAGATTTGCTTGATCGCTTCTACAACG
AAACTGGAATTAAAGTAGTTGCAGATGTTTTTGACTCCAATGAGTCAATGTTAGCTAGGGTCCAAGCAGGGGGGGGTGGA
GCTTATAGTGTCATCTATCCATCTGATTATATGGTACAAAAAATGACTAATCTAAATTTGCTGACGCAGTTAGACCAATC
TCTAATTGTTGGTTTAGACGACTTATTTCCCCAATTTCAAGACCCGATATATGATCCTGGTAATACCCATAGCGTACCCA
TAAGTTGGGGTACTACTGGTTTAATTTACAATAGCAAATTGTTATCTACTCCACCTAATGACTGGAAATATCTCTGGGAA
AATCAAGATTTAGTATTGAAGCGGATGACTTTAGTGAATGATGTACGGGAGGTGATCGGTTGTGCTTTAAGAAAATTAGG
ATATTCTTACAACTCTACCAATGTAGATAACATTAAGGAAGCTTATCAAGAGTTACTCACGCTCAAACCAGCGATCGCTT
CTTTTACTACAGATGGTTGGCGGTCTCAAATTTTGGTAGAAGATTTGTTAATTGCTATGTGTTATTCTTCTGATGCAGCA
GAAATTAAACCAGAAAATGAAGATCTAGAATATATTACCCCACGCAGTGGTTCTTCCCTATGGACAGATACATTAGTAAT
TCCCAAAAATGCTCCTAACCCAGAGGGTGCTTATCAATGGATTAACTTTATGTTACAGCCAGATGTAGCAGCTCAAATTG
TGGAAAGACTAAGTTTTACTACAGCTTCTCGTAAAGCTTATCAAATATTACCAGAAGAACTAAAGGAAGACTCTACTTTA
TTTCCTCCGGAGTCAGTTCTTGAAAAGTGCGAAAGTTTAGCTCCACTGGGTAAATATGAAGAAGTTTATGAAAGATATTG
GACTCAATTAAGAAGTGAATAA

Upstream 100 bases:

>100_bases
TACTGTGCCTCCAACTAATTTACAGAAATACCAAGGCAATGATATAGCTAAATCAATTTATCAGCAAAATACTACCACTT
CTAGACGTAAATTTCTGAAA

Downstream 100 bases:

>100_bases
CTCAAAGAATAAAGAAGATAAATTACTGAATTTATTTATAGGATTATTCTACGAACTTTTTTGACTTATTAAGGTATAGG
TAAAGATTAATAAATTTTCT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSAATITVSTLSGCGWRLAEVKSNPKMRGRSDLLYIYTWAGYTDQDLLDRFYNETGIKVVADVFDSNESMLARVQAGGGG
AYSVIYPSDYMVQKMTNLNLLTQLDQSLIVGLDDLFPQFQDPIYDPGNTHSVPISWGTTGLIYNSKLLSTPPNDWKYLWE
NQDLVLKRMTLVNDVREVIGCALRKLGYSYNSTNVDNIKEAYQELLTLKPAIASFTTDGWRSQILVEDLLIAMCYSSDAA
EIKPENEDLEYITPRSGSSLWTDTLVIPKNAPNPEGAYQWINFMLQPDVAAQIVERLSFTTASRKAYQILPEELKEDSTL
FPPESVLEKCESLAPLGKYEEVYERYWTQLRSE

Sequences:

>Translated_353_residues
MSAATITVSTLSGCGWRLAEVKSNPKMRGRSDLLYIYTWAGYTDQDLLDRFYNETGIKVVADVFDSNESMLARVQAGGGG
AYSVIYPSDYMVQKMTNLNLLTQLDQSLIVGLDDLFPQFQDPIYDPGNTHSVPISWGTTGLIYNSKLLSTPPNDWKYLWE
NQDLVLKRMTLVNDVREVIGCALRKLGYSYNSTNVDNIKEAYQELLTLKPAIASFTTDGWRSQILVEDLLIAMCYSSDAA
EIKPENEDLEYITPRSGSSLWTDTLVIPKNAPNPEGAYQWINFMLQPDVAAQIVERLSFTTASRKAYQILPEELKEDSTL
FPPESVLEKCESLAPLGKYEEVYERYWTQLRSE
>Mature_352_residues
SAATITVSTLSGCGWRLAEVKSNPKMRGRSDLLYIYTWAGYTDQDLLDRFYNETGIKVVADVFDSNESMLARVQAGGGGA
YSVIYPSDYMVQKMTNLNLLTQLDQSLIVGLDDLFPQFQDPIYDPGNTHSVPISWGTTGLIYNSKLLSTPPNDWKYLWEN
QDLVLKRMTLVNDVREVIGCALRKLGYSYNSTNVDNIKEAYQELLTLKPAIASFTTDGWRSQILVEDLLIAMCYSSDAAE
IKPENEDLEYITPRSGSSLWTDTLVIPKNAPNPEGAYQWINFMLQPDVAAQIVERLSFTTASRKAYQILPEELKEDSTLF
PPESVLEKCESLAPLGKYEEVYERYWTQLRSE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=33.4365325077399, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1787078, Length=338, Percent_Identity=28.698224852071, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787711, Length=297, Percent_Identity=23.2323232323232, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39895; Mature: 39764

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATITVSTLSGCGWRLAEVKSNPKMRGRSDLLYIYTWAGYTDQDLLDRFYNETGIKVV
CCCEEEEEEECCCCCEEEEHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEH
ADVFDSNESMLARVQAGGGGAYSVIYPSDYMVQKMTNLNLLTQLDQSLIVGLDDLFPQFQ
HHHHCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHC
DPIYDPGNTHSVPISWGTTGLIYNSKLLSTPPNDWKYLWENQDLVLKRMTLVNDVREVIG
CCCCCCCCCCEEEEECCCEEEEECCEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
CALRKLGYSYNSTNVDNIKEAYQELLTLKPAIASFTTDGWRSQILVEDLLIAMCYSSDAA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
EIKPENEDLEYITPRSGSSLWTDTLVIPKNAPNPEGAYQWINFMLQPDVAAQIVERLSFT
CCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
TASRKAYQILPEELKEDSTLFPPESVLEKCESLAPLGKYEEVYERYWTQLRSE
HHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAATITVSTLSGCGWRLAEVKSNPKMRGRSDLLYIYTWAGYTDQDLLDRFYNETGIKVV
CCEEEEEEECCCCCEEEEHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEH
ADVFDSNESMLARVQAGGGGAYSVIYPSDYMVQKMTNLNLLTQLDQSLIVGLDDLFPQFQ
HHHHCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHC
DPIYDPGNTHSVPISWGTTGLIYNSKLLSTPPNDWKYLWENQDLVLKRMTLVNDVREVIG
CCCCCCCCCCEEEEECCCEEEEECCEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
CALRKLGYSYNSTNVDNIKEAYQELLTLKPAIASFTTDGWRSQILVEDLLIAMCYSSDAA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
EIKPENEDLEYITPRSGSSLWTDTLVIPKNAPNPEGAYQWINFMLQPDVAAQIVERLSFT
CCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
TASRKAYQILPEELKEDSTLFPPESVLEKCESLAPLGKYEEVYERYWTQLRSE
HHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]